Done. -parsing GTF file: /tmp/nxf.VleedfCDzv/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.VleedfCDzv/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.VleedfCDzv/aih-tih-sc-966ec5-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.VleedfCDzv/aih-tih-sc-966ec5-R1_A23YTGFLT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.VleedfCDzv/fi_workdir/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.VleedfCDzv/fi_workdir/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam aih-tih-sc-966ec5-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index aih-tih-sc-966ec5-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam 11:27:18 : INFO : Done. -parsing GTF file: /tmp/nxf.VleedfCDzv/fi_workdir/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.gtf -parsing /tmp/nxf.VleedfCDzv/fi_workdir/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] [140000] [150000] [160000] [170000] [180000] [190000] [200000] [210000] [220000] [230000] [240000] [250000] [260000] [270000] [280000] [290000] [300000] [310000] [320000] [330000] [340000] [350000] [360000] [370000] [380000] [390000] [400000] [410000] [420000] [430000] [440000] [450000] [460000] [470000] [480000] [490000] [500000] [510000] [520000] [530000] [540000] [550000] [560000] [570000] [580000] [590000] [600000] [610000] [620000] [630000] [640000] [650000] [660000] [670000] [680000] [690000] [700000] [710000] [720000] [730000] [740000] [750000] [760000] -done parsing /tmp/nxf.VleedfCDzv/fi_workdir/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'num_hits: 4 != num_counted_on_fusion_contigs 2 ' => 32, 'num_hits: 6 != num_counted_on_fusion_contigs 4 ' => 4, 'num_hits: 6 != num_counted_on_fusion_contigs 3 ' => 24, ' ** passed ** ' => 3968, 'small anchor length' => 14, 'num_hits: 6 != num_counted_on_fusion_contigs 2 ' => 2, 'low complexity anchor region' => 5, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 45, 'num genes matched < 2' => 76, 'excessive soft clipping' => 5967, 'per_id < 96' => 2875 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.VleedfCDzv/fi_workdir/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] -fusion SPANNING read extraction for scaff: AC006197.2--NKAIN2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] [109000] [110000] [111000] [112000] [113000] [114000] [115000] [116000] [117000] [118000] [119000] [120000] [121000] [122000] [123000] [124000] [125000] [126000] [127000] [128000] [129000] [130000] [131000] [132000] [133000] [134000] [135000] [136000] [137000] [138000] [139000] [140000] [141000] [142000] [143000] [144000] [145000] [146000] [147000] [148000] [149000] [150000] [151000] [152000] [153000] [154000] [155000] [156000] [157000] [158000] [159000] [160000] [161000] [162000] [163000] [164000] [165000] [166000] [167000] [168000] [169000] [170000] [171000] [172000] [173000] [174000] [175000] [176000] [177000] [178000] [179000] [180000] [181000] [182000] [183000] [184000] [185000] [186000] [187000] [188000] [189000] [190000] [191000] [192000] [193000] [194000] [195000] [196000] [197000] [198000] [199000] [200000] [201000] [202000] [203000] [204000] [205000] [206000] [207000] [208000] [209000] [210000] [211000] [212000] [213000] [214000] [215000] [216000] [217000] [218000] [219000] [220000] [221000] [222000] [223000] [224000] [225000] [226000] [227000] [228000] [229000] [230000] [231000] [232000] [233000] [234000] [235000] [236000] [237000] [238000] [239000] [240000] [241000] [242000] [243000] [244000] [245000] [246000] [247000] [248000] [249000] [250000] [251000] [252000] [253000] [254000] [255000] [256000] [257000] [258000] [259000] [260000] [261000] [262000] [263000] [264000] [265000] [266000] [267000] [268000] [269000] [270000] [271000] [272000] [273000] [274000] [275000] [276000] [277000] [278000] [279000] [280000] [281000] [282000] [283000] [284000] [285000] [286000] [287000] [288000] [289000] [290000] [291000] [292000] [293000] [294000] [295000] [296000] [297000] [298000] [299000] [300000] [301000] [302000] [303000] [304000] [305000] [306000] [307000] [308000] [309000] [310000] [311000] [312000] [313000] [314000] [315000] [316000] [317000] [318000] [319000] [320000] [321000] [322000] [323000] [324000] [325000] [326000] [327000] [328000] [329000] [330000] [331000] [332000] [333000] [334000] [335000] [336000] [337000] [338000] [339000] [340000] [341000] [342000] -fusion SPANNING read extraction for scaff: AC136431.1--PKD1P5 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] -fusion SPANNING read extraction for scaff: ENOPH1--AC002451.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] -fusion SPANNING read extraction for scaff: ENOPH1--DYNC1I1 [1000] [2000] [3000] [4000] [5000] -fusion SPANNING read extraction for scaff: GUCY1B2--NKAIN2 [1000] [2000] [3000] [4000] [5000] -fusion SPANNING read extraction for scaff: LINC00381--NKAIN2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] -fusion SPANNING read extraction for scaff: PCDHA4--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] -fusion SPANNING read extraction for scaff: PCDHA4--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] -fusion SPANNING read extraction for scaff: PCDHA4--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] -fusion SPANNING read extraction for scaff: PTPN20--BMS1P7 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] [109000] [110000] [111000] -fusion SPANNING read extraction for scaff: WAC--AC023141.7 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] [109000] [110000] [111000] -fusion SPANNING read extraction for scaff: WAC--AC128676.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] -fusion SPANNING read extraction for scaff: ZNF66--ZNF626 -filtered reads reasons: $VAR1 = { 'lacks exon overlap' => 30566 }; EM: Starting log likelihood: -3160.781060 EM: Round [1] log likelihood: -3147.275592 EM: Round [2] log likelihood: -3141.923629 EM: Round [3] log likelihood: -3139.613174 EM: Round [4] log likelihood: -3138.384885 EM: Round [5] log likelihood: -3137.537748 EM: Round [6] log likelihood: -3136.849239 EM: Round [7] log likelihood: -3136.252073 EM: Round [8] log likelihood: -3135.723109 EM: Round [9] log likelihood: -3135.251283 EM: Round [10] log likelihood: -3134.829226 EM: Round [11] log likelihood: -3134.451113 EM: Round [12] log likelihood: -3134.112029 EM: Round [13] log likelihood: -3133.807735 EM: Round [14] log likelihood: -3133.534536 EM: Round [15] log likelihood: -3133.289186 EM: Round [16] log likelihood: -3133.068825 EM: Round [17] log likelihood: -3132.870919 EM: Round [18] log likelihood: -3132.693215 EM: Round [19] log likelihood: -3132.533703 EM: Round [20] log likelihood: -3132.390583 EM: Round [21] log likelihood: -3132.262244 EM: Round [22] log likelihood: -3132.147233 EM: Round [23] log likelihood: -3132.044243 EM: Round [24] log likelihood: -3131.952093 EM: Round [25] log likelihood: -3131.869718 EM: Round [26] log likelihood: -3131.796151 EM: Round [27] log likelihood: -3131.730518 EM: Round [28] log likelihood: -3131.672027 EM: Round [29] log likelihood: -3131.619959 EM: Round [30] log likelihood: -3131.573663 EM: Round [31] log likelihood: -3131.532548 EM: Round [32] log likelihood: -3131.496080 EM: Round [33] log likelihood: -3131.463772 EM: Round [34] log likelihood: -3131.435186 EM: Round [35] log likelihood: -3131.409925 EM: Round [36] log likelihood: -3131.387631 EM: Round [37] log likelihood: -3131.367980 EM: Round [38] log likelihood: -3131.350679 EM: Round [39] log likelihood: -3131.335467 EM: Round [40] log likelihood: -3131.322107 EM: Round [41] log likelihood: -3131.310388 EM: Round [42] log likelihood: -3131.300121 EM: Round [43] log likelihood: -3131.291135 EM: Round [44] log likelihood: -3131.283280 EM: Round [45] log likelihood: -3131.276420 EM: Round [46] log likelihood: -3131.270436 EM: Round [47] log likelihood: -3131.265221 EM: Round [48] log likelihood: -3131.260680 EM: Round [49] log likelihood: -3131.256730 EM: Round [50] log likelihood: -3131.253297 EM: Round [51] log likelihood: -3131.250317 EM: Round [52] log likelihood: -3131.247730 EM: Round [53] log likelihood: -3131.245488 EM: Round [54] log likelihood: -3131.243545 EM: Round [55] log likelihood: -3131.241863 EM: Round [56] log likelihood: -3131.240408 EM: Round [57] log likelihood: -3131.239150 EM: Round [58] log likelihood: -3131.238063 EM: Round [59] log likelihood: -3131.237123 EM: Round [60] log likelihood: -3131.236313 EM: Round [61] log likelihood: -3131.235613 EM: Round [62] log likelihood: -3131.235010 EM: Round [63] log likelihood: -3131.234490 EM: Round [64] log likelihood: -3131.234042 EM: Round [65] log likelihood: -3131.233656 EM: Round [66] log likelihood: -3131.233323 EM: Round [67] log likelihood: -3131.233037 EM: Round [68] log likelihood: -3131.232791 EM: Round [69] log likelihood: -3131.232579 EM: Round [70] log likelihood: -3131.232397 EM: Round [71] log likelihood: -3131.232240 EM: Round [72] log likelihood: -3131.232106 EM: Round [73] log likelihood: -3131.231990 EM: Round [74] log likelihood: -3131.231891 EM: Stopping iterations at round 74 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.VleedfCDzv/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.consolidated.bam into /tmp/nxf.VleedfCDzv/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.VleedfCDzv/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.VleedfCDzv/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.consolidated.bam.read_coords > /tmp/nxf.VleedfCDzv/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.VleedfCDzv/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.consolidated.bam.frag_coords > /tmp/nxf.VleedfCDzv/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.VleedfCDzv/fi_workdir/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.VleedfCDzv/fi_workdir/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.VleedfCDzv/fi_workdir/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.VleedfCDzv/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.VleedfCDzv/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -3015.222079 EM: Round [1] log likelihood: -3001.716611 EM: Round [2] log likelihood: -2996.364649 EM: Round [3] log likelihood: -2994.054193 EM: Round [4] log likelihood: -2992.825904 EM: Round [5] log likelihood: -2991.978768 EM: Round [6] log likelihood: -2991.290258 EM: Round [7] log likelihood: -2990.693092 EM: Round [8] log likelihood: -2990.164128 EM: Round [9] log likelihood: -2989.692302 EM: Round [10] log likelihood: -2989.270245 EM: Round [11] log likelihood: -2988.892132 EM: Round [12] log likelihood: -2988.553048 EM: Round [13] log likelihood: -2988.248755 EM: Round [14] log likelihood: -2987.975555 EM: Round [15] log likelihood: -2987.730205 EM: Round [16] log likelihood: -2987.509844 EM: Round [17] log likelihood: -2987.311938 EM: Round [18] log likelihood: -2987.134234 EM: Round [19] log likelihood: -2986.974722 EM: Round [20] log likelihood: -2986.831603 EM: Round [21] log likelihood: -2986.703263 EM: Round [22] log likelihood: -2986.588252 EM: Round [23] log likelihood: -2986.485262 EM: Round [24] log likelihood: -2986.393112 EM: Round [25] log likelihood: -2986.310737 EM: Round [26] log likelihood: -2986.237170 EM: Round [27] log likelihood: -2986.171537 EM: Round [28] log likelihood: -2986.113046 EM: Round [29] log likelihood: -2986.060978 EM: Round [30] log likelihood: -2986.014682 EM: Round [31] log likelihood: -2985.973567 EM: Round [32] log likelihood: -2985.937099 EM: Round [33] log likelihood: -2985.904791 EM: Round [34] log likelihood: -2985.876205 EM: Round [35] log likelihood: -2985.850945 EM: Round [36] log likelihood: -2985.828650 EM: Round [37] log likelihood: -2985.808999 EM: Round [38] log likelihood: -2985.791698 EM: Round [39] log likelihood: -2985.776486 EM: Round [40] log likelihood: -2985.763126 EM: Round [41] log likelihood: -2985.751408 EM: Round [42] log likelihood: -2985.741140 EM: Round [43] log likelihood: -2985.732154 EM: Round [44] log likelihood: -2985.724299 EM: Round [45] log likelihood: -2985.717439 EM: Round [46] log likelihood: -2985.711455 EM: Round [47] log likelihood: -2985.706240 EM: Round [48] log likelihood: -2985.701699 EM: Round [49] log likelihood: -2985.697749 EM: Round [50] log likelihood: -2985.694317 EM: Round [51] log likelihood: -2985.691336 EM: Round [52] log likelihood: -2985.688749 EM: Round [53] log likelihood: -2985.686507 EM: Round [54] log likelihood: -2985.684564 EM: Round [55] log likelihood: -2985.682882 EM: Round [56] log likelihood: -2985.681427 EM: Round [57] log likelihood: -2985.680169 EM: Round [58] log likelihood: -2985.679082 EM: Round [59] log likelihood: -2985.678142 EM: Round [60] log likelihood: -2985.677332 EM: Round [61] log likelihood: -2985.676632 EM: Round [62] log likelihood: -2985.676029 EM: Round [63] log likelihood: -2985.675509 EM: Round [64] log likelihood: -2985.675061 EM: Round [65] log likelihood: -2985.674675 EM: Round [66] log likelihood: -2985.674342 EM: Round [67] log likelihood: -2985.674056 EM: Round [68] log likelihood: -2985.673810 EM: Round [69] log likelihood: -2985.673598 EM: Round [70] log likelihood: -2985.673416 EM: Round [71] log likelihood: -2985.673259 EM: Round [72] log likelihood: -2985.673125 EM: Round [73] log likelihood: -2985.673009 EM: Round [74] log likelihood: -2985.672910 EM: Stopping iterations at round 74 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.VleedfCDzv/aih-tih-sc-966ec5-R1_A23YTGFLT4_1_1.fastp.fastq.gz: 295708708 Warning - not locating file: /tmp/nxf.VleedfCDzv/aih-tih-sc-966ec5-R1_A23YTGFLT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000270025.2] WARNING, no entry stored in dbm for [ENSG00000270025.2] WARNING, no entry stored in dbm for [ENSG00000270025.2] WARNING, no entry stored in dbm for [ENSG00000270025.2] WARNING, no entry stored in dbm for [ENSG00000270025.2] WARNING, no entry stored in dbm for [ENSG00000226240.2] WARNING, no entry stored in dbm for [ENSG00000286591.1] WARNING, no entry stored in dbm for [ENSG00000123201.14] WARNING, no entry stored in dbm for [ENSG00000123201.14] WARNING, no entry stored in dbm for [ENSG00000260126.2] WARNING, no entry stored in dbm for [ENSG00000254681.6] WARNING, no entry stored in dbm for [ENSG00000280136.2] WARNING, no entry stored in dbm for [ENSG00000226049.3] WARNING, no entry stored in dbm for [ENSG00000260404.3] ls: cannot access 'IGV_inputs': No such file or directory