Done. -parsing GTF file: /tmp/nxf.BAl7gZ6CTL/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.BAl7gZ6CTL/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.BAl7gZ6CTL/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.BAl7gZ6CTL/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.BAl7gZ6CTL/fi_workdir/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.BAl7gZ6CTL/fi_workdir/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam 11:37:08 : INFO : Done. -parsing GTF file: /tmp/nxf.BAl7gZ6CTL/fi_workdir/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.gtf -parsing /tmp/nxf.BAl7gZ6CTL/fi_workdir/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] [140000] [150000] [160000] [170000] [180000] [190000] [200000] [210000] [220000] [230000] [240000] [250000] [260000] [270000] [280000] [290000] [300000] [310000] [320000] [330000] [340000] [350000] [360000] [370000] [380000] [390000] [400000] [410000] [420000] [430000] [440000] [450000] [460000] [470000] [480000] [490000] [500000] [510000] [520000] [530000] [540000] [550000] [560000] [570000] [580000] [590000] [600000] [610000] [620000] [630000] [640000] [650000] [660000] [670000] [680000] [690000] [700000] [710000] [720000] [730000] [740000] [750000] [760000] [770000] [780000] -done parsing /tmp/nxf.BAl7gZ6CTL/fi_workdir/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'seq-similar region overlap' => 232, 'num genes matched < 2' => 12, 'small anchor length' => 3, 'num_hits: 4 != num_counted_on_fusion_contigs 1 ' => 6, 'num_hits: 6 != num_counted_on_fusion_contigs 3 ' => 33, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 46, 'num_hits: 4 != num_counted_on_fusion_contigs 2 ' => 24, ' ** passed ** ' => 2339, 'per_id < 96' => 2928, 'num_hits: 4 != num_counted_on_fusion_contigs 3 ' => 6, 'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 3, 'excessive soft clipping' => 4891, 'low complexity anchor region' => 31, 'num_hits: 3 != num_counted_on_fusion_contigs 2 ' => 4 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.BAl7gZ6CTL/fi_workdir/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] -fusion SPANNING read extraction for scaff: AC020899.1--FLT3LG [1000] [2000] [3000] [4000] -fusion SPANNING read extraction for scaff: AC092807.3--DDAH1 [1000] [2000] [3000] [4000] -fusion SPANNING read extraction for scaff: ASAH2B--PRKG1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] -fusion SPANNING read extraction for scaff: CSMD3--RNU4-37P [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] -fusion SPANNING read extraction for scaff: CSMD3--LINC02237 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] [109000] -fusion SPANNING read extraction for scaff: GDPD1--SETD2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] -fusion SPANNING read extraction for scaff: GUSBP13--NAIP [1000] [2000] -fusion SPANNING read extraction for scaff: KLRC4--KLRK1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] -fusion SPANNING read extraction for scaff: PARD6B--RASSF2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] [109000] [110000] [111000] -fusion SPANNING read extraction for scaff: SETD2--CA4 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] -fusion SPANNING read extraction for scaff: SFMBT1--RFT1 [1000] [2000] -fusion SPANNING read extraction for scaff: SORCS1--AL353740.1 [1000] [2000] -fusion SPANNING read extraction for scaff: SORCS1--LINC02661 [1000] [2000] [3000] [4000] -fusion SPANNING read extraction for scaff: SREK1IP1--RGS7BP [1000] [2000] [3000] -fusion SPANNING read extraction for scaff: SREK1IP1--MRPL49P1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1 [1000] [2000] [3000] [4000] [5000] [6000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] -fusion SPANNING read extraction for scaff: TTC39C--ISM1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] -fusion SPANNING read extraction for scaff: TTC39C--AL121782.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 -filtered reads reasons: $VAR1 = { 'lacks exon overlap' => 3446, 'seq similar region alignment' => 25791 }; EM: Starting log likelihood: -2751.836266 EM: Round [1] log likelihood: -2751.064566 EM: Round [2] log likelihood: -2750.426526 EM: Round [3] log likelihood: -2749.882730 EM: Round [4] log likelihood: -2749.416482 EM: Round [5] log likelihood: -2749.014938 EM: Round [6] log likelihood: -2748.667828 EM: Round [7] log likelihood: -2748.366831 EM: Round [8] log likelihood: -2748.105122 EM: Round [9] log likelihood: -2747.877056 EM: Round [10] log likelihood: -2747.677917 EM: Round [11] log likelihood: -2747.503744 EM: Round [12] log likelihood: -2747.351186 EM: Round [13] log likelihood: -2747.217391 EM: Round [14] log likelihood: -2747.099922 EM: Round [15] log likelihood: -2746.996689 EM: Round [16] log likelihood: -2746.905892 EM: Round [17] log likelihood: -2746.825974 EM: Round [18] log likelihood: -2746.755586 EM: Round [19] log likelihood: -2746.693557 EM: Round [20] log likelihood: -2746.638868 EM: Round [21] log likelihood: -2746.590630 EM: Round [22] log likelihood: -2746.548066 EM: Round [23] log likelihood: -2746.510495 EM: Round [24] log likelihood: -2746.477322 EM: Round [25] log likelihood: -2746.448025 EM: Round [26] log likelihood: -2746.422145 EM: Round [27] log likelihood: -2746.399279 EM: Round [28] log likelihood: -2746.379072 EM: Round [29] log likelihood: -2746.361212 EM: Round [30] log likelihood: -2746.345425 EM: Round [31] log likelihood: -2746.331468 EM: Round [32] log likelihood: -2746.319127 EM: Round [33] log likelihood: -2746.308215 EM: Round [34] log likelihood: -2746.298566 EM: Round [35] log likelihood: -2746.290031 EM: Round [36] log likelihood: -2746.282484 EM: Round [37] log likelihood: -2746.275807 EM: Round [38] log likelihood: -2746.269902 EM: Round [39] log likelihood: -2746.264678 EM: Round [40] log likelihood: -2746.260057 EM: Round [41] log likelihood: -2746.255969 EM: Round [42] log likelihood: -2746.252353 EM: Round [43] log likelihood: -2746.249153 EM: Round [44] log likelihood: -2746.246322 EM: Round [45] log likelihood: -2746.243818 EM: Round [46] log likelihood: -2746.241602 EM: Round [47] log likelihood: -2746.239641 EM: Round [48] log likelihood: -2746.237906 EM: Round [49] log likelihood: -2746.236372 EM: Round [50] log likelihood: -2746.235014 EM: Round [51] log likelihood: -2746.233812 EM: Round [52] log likelihood: -2746.232749 EM: Round [53] log likelihood: -2746.231808 EM: Round [54] log likelihood: -2746.230975 EM: Round [55] log likelihood: -2746.230239 EM: Round [56] log likelihood: -2746.229587 EM: Round [57] log likelihood: -2746.229010 EM: Round [58] log likelihood: -2746.228500 EM: Round [59] log likelihood: -2746.228048 EM: Round [60] log likelihood: -2746.227649 EM: Round [61] log likelihood: -2746.227295 EM: Round [62] log likelihood: -2746.226982 EM: Round [63] log likelihood: -2746.226705 EM: Round [64] log likelihood: -2746.226460 EM: Round [65] log likelihood: -2746.226244 EM: Round [66] log likelihood: -2746.226052 EM: Round [67] log likelihood: -2746.225882 EM: Round [68] log likelihood: -2746.225732 EM: Round [69] log likelihood: -2746.225599 EM: Round [70] log likelihood: -2746.225481 EM: Round [71] log likelihood: -2746.225377 EM: Round [72] log likelihood: -2746.225285 EM: Stopping iterations at round 72 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.BAl7gZ6CTL/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.consolidated.bam into /tmp/nxf.BAl7gZ6CTL/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.BAl7gZ6CTL/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.BAl7gZ6CTL/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.consolidated.bam.read_coords > /tmp/nxf.BAl7gZ6CTL/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.BAl7gZ6CTL/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.consolidated.bam.frag_coords > /tmp/nxf.BAl7gZ6CTL/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.BAl7gZ6CTL/fi_workdir/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.BAl7gZ6CTL/fi_workdir/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.BAl7gZ6CTL/fi_workdir/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.BAl7gZ6CTL/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.BAl7gZ6CTL/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -2574.397102 EM: Round [1] log likelihood: -2574.377943 EM: Round [2] log likelihood: -2574.377526 EM: Round [3] log likelihood: -2574.377517 EM: Stopping iterations at round 3 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.BAl7gZ6CTL/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1_1.fastp.fastq.gz: 296382314 Warning - not locating file: /tmp/nxf.BAl7gZ6CTL/aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000242858.1] WARNING, no entry stored in dbm for [ENSG00000198237.8] WARNING, no entry stored in dbm for [ENSG00000280136.2] WARNING, no entry stored in dbm for [ENSG00000226049.3] WARNING, no entry stored in dbm for [ENSG00000260404.3] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] ls: cannot access 'IGV_inputs': No such file or directory