+ mkdir aih-tih-sc-28eb14-R1_A23YTGFLT4_1 + all_qc -bam aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-28eb14-R1_A23YTGFLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai 0it [00:00, ?it/s] 140606it [00:00, 1405933.96it/s] 285982it [00:00, 1434002.34it/s] 442285it [00:00, 1492871.62it/s] 603023it [00:00, 1538039.16it/s] 766022it [00:00, 1571104.53it/s] 931361it [00:00, 1599046.58it/s] 1098218it [00:00, 1621597.64it/s] 1263347it [00:00, 1631015.03it/s] 1429150it [00:00, 1639429.47it/s] 1596019it [00:01, 1648433.47it/s] 1760863it [00:01, 1647850.41it/s] 1925649it [00:01, 1638981.24it/s] 2090161it [00:01, 1640805.66it/s] 2255206it [00:01, 1643518.70it/s] 2420915it [00:01, 1647550.89it/s] 2594081it [00:01, 1672801.86it/s] 2762072it [00:01, 1674836.82it/s] 2929561it [00:01, 1670970.34it/s] 3096664it [00:01, 1655804.46it/s] 3262277it [00:02, 1633430.68it/s] 3429448it [00:02, 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1662116.64it/s] 8248409it [00:05, 1567006.83it/s] 8406238it [00:05, 1493023.93it/s] 8570306it [00:05, 1534450.86it/s] 8732736it [00:05, 1560137.06it/s] 8899808it [00:05, 1592206.72it/s] 9065502it [00:05, 1611149.95it/s] 9228537it [00:05, 1616794.18it/s] 9397590it [00:05, 1638637.14it/s] 9561775it [00:06, 1620781.57it/s] 9727364it [00:06, 1631141.79it/s] 9891435it [00:06, 1633797.61it/s] 10054954it [00:06, 1630241.91it/s] 10220586it [00:06, 1637873.02it/s] 10385977it [00:06, 1642633.15it/s] 10550294it [00:06, 1641098.83it/s] 10714441it [00:06, 1628500.99it/s] 10877336it [00:06, 1568922.19it/s] 11034693it [00:06, 1519358.44it/s] 11187170it [00:07, 1475903.11it/s] 11335248it [00:07, 1473627.80it/s] 11482941it [00:07, 1445995.31it/s] 11638274it [00:07, 1476860.62it/s] 11803413it [00:07, 1527678.11it/s] 11969649it [00:07, 1567222.34it/s] 12126711it [00:07, 1563612.33it/s] 12283307it [00:07, 1553247.68it/s] 12450791it [00:07, 1589102.94it/s] 12616595it [00:07, 1609560.73it/s] 12781668it [00:08, 1621805.97it/s] 12943953it [00:08, 1596800.93it/s] 13109710it [00:08, 1614743.58it/s] 13271315it [00:08, 1610923.02it/s] 13436760it [00:08, 1623835.85it/s] 13602345it [00:08, 1633349.32it/s] 13765738it [00:08, 1621573.85it/s] 13930532it [00:08, 1629346.50it/s] 14093512it [00:08, 1613716.11it/s] 14257034it [00:09, 1620066.91it/s] 14420396it [00:09, 1624000.87it/s] 14582833it [00:09, 1623506.28it/s] 14748544it [00:09, 1633515.72it/s] 14911918it [00:09, 1631842.14it/s] 15076682it [00:09, 1636543.31it/s] 15240349it [00:09, 1634785.64it/s] 15410865it [00:09, 1655763.88it/s] 15505077it [00:09, 1588817.71it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md.bai + merge_qc -folders aih-tih-sc-28eb14-R1_A23YTGFLT4_1/aih-tih-sc-28eb14-R1_A23YTGFLT4_1.md -output aih-tih-sc-28eb14-R1_A23YTGFLT4_1/aih-tih-sc-28eb14-R1_A23YTGFLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-28eb14-R1_A23YTGFLT4_1/aih-tih-sc-28eb14-R1_A23YTGFLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-28eb14-R1_A23YTGFLT4_1/aih-tih-sc-28eb14-R1_A23YTGFLT4_1_full_basic_stats.tsv