File Info

Filename
.command.err
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/67/bf423cc783fa32937cbd4368eada68/.command.err
Size
22.1 KB
Attempt
Done.
-parsing GTF file: /tmp/nxf.i1GpavJT2R/aih-tih-sc-776623-R1_A23YTGFLT4_1.gtf
* Running CMD: /usr/local/bin/STAR  --runThreadN 8  --genomeDir /tmp/nxf.i1GpavJT2R/ctat_genome_lib_build_dir/ref_genome.fa.star.idx  --outSAMtype BAM SortedByCoordinate  --twopassMode None  --alignSJDBoverhangMin 10  --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705  --alignInsertionFlush Right   --alignMatesGapMax 100000  --alignIntronMax 100000  --outSAMattributes All  --readFilesIn /tmp/nxf.i1GpavJT2R/aih-tih-sc-776623-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.i1GpavJT2R/aih-tih-sc-776623-R1_A23YTGFLT4_1_2.fastp.fastq.gz  --genomeFastaFiles /tmp/nxf.i1GpavJT2R/fi_workdir/aih-tih-sc-776623-R1_A23YTGFLT4_1.fa  --outSAMfilter KeepAllAddedReferences  --sjdbGTFfile /tmp/nxf.i1GpavJT2R/fi_workdir/aih-tih-sc-776623-R1_A23YTGFLT4_1.gtf  --alignSJstitchMismatchNmax 5 -1 5 5  --scoreGapNoncan -6  --readFilesCommand 'gunzip -c' 
* Running CMD: mv Aligned.sortedByCoord.out.bam aih-tih-sc-776623-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam
* Running CMD: samtools index aih-tih-sc-776623-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam
12:19:32 : INFO : Done.
-parsing GTF file: /tmp/nxf.i1GpavJT2R/fi_workdir/aih-tih-sc-776623-R1_A23YTGFLT4_1.gtf
-parsing /tmp/nxf.i1GpavJT2R/fi_workdir/aih-tih-sc-776623-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam

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[1000000]   -done parsing /tmp/nxf.i1GpavJT2R/fi_workdir/aih-tih-sc-776623-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.  Extracting junction info.
junction read elimination tally: $VAR1 = {
          'seq-similar region overlap' => 64,
          'num genes matched < 2' => 51,
          'low complexity anchor region' => 15,
          'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 3,
          'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 94,
          'per_id < 96' => 3109,
          ' ** passed ** ' => 6975,
          'num_hits: 4 != num_counted_on_fusion_contigs 2 ' => 8,
          'small anchor length' => 12,
          'num_hits: 3 != num_counted_on_fusion_contigs 2 ' => 2,
          'excessive soft clipping' => 6780,
          'exons hit < 2' => 2
        };
-writing fusion junction support info.
-outputting the spanning read info: /tmp/nxf.i1GpavJT2R/fi_workdir/aih-tih-sc-776623-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info.
 - counting read alignments among fusion contigs.

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[9000]   -fusion SPANNING read extraction for scaff: AC092666.2--ACTR3B

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[7000]   -fusion SPANNING read extraction for scaff: AP001347.1--ANKRD20A11P

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[92000]   -fusion SPANNING read extraction for scaff: FAM53B--CTBP2

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[12000]   -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC1

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[11000]   -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC2

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[12000]   -fusion SPANNING read extraction for scaff: PCDHA11--PCDHA13

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[12000]   -fusion SPANNING read extraction for scaff: PCDHA6--PCDHAC1

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[11000]   -fusion SPANNING read extraction for scaff: PCDHA6--PCDHAC2

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[12000]   -fusion SPANNING read extraction for scaff: PCDHA6--PCDHA13

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[8000]   -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGC4

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[9000]   -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGC5

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[59000]   -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGC3

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[16000]   -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGB6

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[26000]   -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA10

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[22000]   -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGB7

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[22000]   -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA11

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[26000]   -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA12

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[15000]   -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA9

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[167000]   -fusion SPANNING read extraction for scaff: TELO2--TPSAB1

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[32000]   -fusion SPANNING read extraction for scaff: TLK2--FAM157A

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[8000]   -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1

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[100000]   -fusion SPANNING read extraction for scaff: TNRC6B--DLGAP4-AS1

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[21000]   -fusion SPANNING read extraction for scaff: TVP23C--CDRT4
-filtered reads reasons: $VAR1 = {
          'seq similar region alignment' => 35870,
          'lacks exon overlap' => 9238
        };
EM: Starting log likelihood: -3393.431361
EM: Round [1] log likelihood: -3392.871833
EM: Round [2] log likelihood: -3392.792958
EM: Round [3] log likelihood: -3392.726138
EM: Round [4] log likelihood: -3392.668567
EM: Round [5] log likelihood: -3392.618746
EM: Round [6] log likelihood: -3392.575478
EM: Round [7] log likelihood: -3392.537786
EM: Round [8] log likelihood: -3392.504867
EM: Round [9] log likelihood: -3392.476052
EM: Round [10] log likelihood: -3392.450781
EM: Round [11] log likelihood: -3392.428582
EM: Round [12] log likelihood: -3392.409053
EM: Round [13] log likelihood: -3392.391851
EM: Round [14] log likelihood: -3392.376682
EM: Round [15] log likelihood: -3392.363293
EM: Round [16] log likelihood: -3392.351465
EM: Round [17] log likelihood: -3392.341009
EM: Round [18] log likelihood: -3392.331759
EM: Round [19] log likelihood: -3392.323571
EM: Round [20] log likelihood: -3392.316320
EM: Round [21] log likelihood: -3392.309896
EM: Round [22] log likelihood: -3392.304201
EM: Round [23] log likelihood: -3392.299151
EM: Round [24] log likelihood: -3392.294672
EM: Round [25] log likelihood: -3392.290698
EM: Round [26] log likelihood: -3392.287171
EM: Round [27] log likelihood: -3392.284040
EM: Round [28] log likelihood: -3392.281260
EM: Round [29] log likelihood: -3392.278791
EM: Round [30] log likelihood: -3392.276597
EM: Round [31] log likelihood: -3392.274649
EM: Round [32] log likelihood: -3392.272917
EM: Round [33] log likelihood: -3392.271378
EM: Round [34] log likelihood: -3392.270009
EM: Round [35] log likelihood: -3392.268793
EM: Round [36] log likelihood: -3392.267711
EM: Round [37] log likelihood: -3392.266749
EM: Round [38] log likelihood: -3392.265893
EM: Round [39] log likelihood: -3392.265131
EM: Round [40] log likelihood: -3392.264453
EM: Round [41] log likelihood: -3392.263849
EM: Round [42] log likelihood: -3392.263312
EM: Round [43] log likelihood: -3392.262834
EM: Round [44] log likelihood: -3392.262407
EM: Round [45] log likelihood: -3392.262027
EM: Round [46] log likelihood: -3392.261689
EM: Round [47] log likelihood: -3392.261387
EM: Round [48] log likelihood: -3392.261117
EM: Round [49] log likelihood: -3392.260876
EM: Round [50] log likelihood: -3392.260662
EM: Round [51] log likelihood: -3392.260470
EM: Round [52] log likelihood: -3392.260298
EM: Round [53] log likelihood: -3392.260144
EM: Round [54] log likelihood: -3392.260007
EM: Round [55] log likelihood: -3392.259884
EM: Round [56] log likelihood: -3392.259773
EM: Round [57] log likelihood: -3392.259674
EM: Stopping iterations at round 57 due to insufficient improvement in likelihood.
[bam_sort_core] merging from 0 files and 8 in-memory blocks...
-extracting read coordinates from /tmp/nxf.i1GpavJT2R/aih-tih-sc-776623-R1_A23YTGFLT4_1.consolidated.bam into /tmp/nxf.i1GpavJT2R/aih-tih-sc-776623-R1_A23YTGFLT4_1.consolidated.bam.read_coords

CMD: touch /tmp/nxf.i1GpavJT2R/aih-tih-sc-776623-R1_A23YTGFLT4_1.consolidated.bam.read_coords.ok
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.i1GpavJT2R/aih-tih-sc-776623-R1_A23YTGFLT4_1.consolidated.bam.read_coords > /tmp/nxf.i1GpavJT2R/aih-tih-sc-776623-R1_A23YTGFLT4_1.consolidated.bam.read_coords.sort_by_readname
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.i1GpavJT2R/aih-tih-sc-776623-R1_A23YTGFLT4_1.consolidated.bam.frag_coords > /tmp/nxf.i1GpavJT2R/aih-tih-sc-776623-R1_A23YTGFLT4_1.consolidated.bam.frag_coords.coord_sorted
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.i1GpavJT2R/fi_workdir/aih-tih-sc-776623-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.i1GpavJT2R/fi_workdir/aih-tih-sc-776623-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter  --genome_lib_dir ctat_genome_lib_build_dir  --max_promiscuity 10  --min_pct_dom_promiscuity 50 
CMD: cp /tmp/nxf.i1GpavJT2R/fi_workdir/aih-tih-sc-776623-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.i1GpavJT2R/aih-tih-sc-776623-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter
-done, see /tmp/nxf.i1GpavJT2R/aih-tih-sc-776623-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass
EM: Starting log likelihood: -3042.398978
EM: Round [1] log likelihood: -3042.337436
EM: Round [2] log likelihood: -3042.336684
EM: Round [3] log likelihood: -3042.336672
EM: Stopping iterations at round 3 due to insufficient improvement in likelihood.
-total frags in /tmp/nxf.i1GpavJT2R/aih-tih-sc-776623-R1_A23YTGFLT4_1_1.fastp.fastq.gz: 296801809
Warning - not locating file: /tmp/nxf.i1GpavJT2R/aih-tih-sc-776623-R1_A23YTGFLT4_1.gmap_trinity_GG.fusions.gff3.bed
WARNING, no entry stored in dbm for [ENSG00000226049.3]
WARNING, no entry stored in dbm for [ENSG00000260404.3]
WARNING, no entry stored in dbm for [ENSG00000236438.7]
WARNING, no entry stored in dbm for [ENSG00000276538.1]
WARNING, no entry stored in dbm for [ENSG00000276538.1]
WARNING, no entry stored in dbm for [ENSG00000276538.1]
WARNING, no entry stored in dbm for [ENSG00000232907.8]
WARNING, no entry stored in dbm for [ENSG00000232907.8]
WARNING, no entry stored in dbm for [ENSG00000232907.8]
WARNING, no entry stored in dbm for [ENSG00000232907.8]
WARNING, no entry stored in dbm for [ENSG00000232907.8]
WARNING, no entry stored in dbm for [ENSG00000232907.8]
WARNING, no entry stored in dbm for [ENSG00000224905.7]
WARNING, no entry stored in dbm for [ENSG00000215559.8]
WARNING, no entry stored in dbm for [ENSG00000224905.7]
WARNING, no entry stored in dbm for [ENSG00000215559.8]
WARNING, no entry stored in dbm for [ENSG00000224905.7]
WARNING, no entry stored in dbm for [ENSG00000215559.8]
WARNING, no entry stored in dbm for [ENSG00000224905.7]
WARNING, no entry stored in dbm for [ENSG00000215559.8]
WARNING, no entry stored in dbm for [ENSG00000224905.7]
WARNING, no entry stored in dbm for [ENSG00000215559.8]
WARNING, no entry stored in dbm for [ENSG00000224905.7]
WARNING, no entry stored in dbm for [ENSG00000215559.8]
WARNING, no entry stored in dbm for [ENSG00000224905.7]
WARNING, no entry stored in dbm for [ENSG00000215559.8]
ls: cannot access 'IGV_inputs': No such file or directory