[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3270ab-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16289863) splicing reads. 266462 splicing junctions. 189990 known splicing junctions. 76472 novel splicing junctions.
sampling 10% (32579726) splicing reads. 326538 splicing junctions. 202964 known splicing junctions. 123574 novel splicing junctions.
sampling 15% (48869589) splicing reads. 370070 splicing junctions. 210125 known splicing junctions. 159945 novel splicing junctions.
sampling 20% (65159453) splicing reads. 404688 splicing junctions. 214950 known splicing junctions. 189738 novel splicing junctions.
sampling 25% (81449316) splicing reads. 433462 splicing junctions. 218595 known splicing junctions. 214867 novel splicing junctions.
sampling 30% (97739179) splicing reads. 457886 splicing junctions. 221270 known splicing junctions. 236616 novel splicing junctions.
sampling 35% (114029042) splicing reads. 478713 splicing junctions. 223388 known splicing junctions. 255325 novel splicing junctions.
sampling 40% (130318906) splicing reads. 497096 splicing junctions. 225068 known splicing junctions. 272028 novel splicing junctions.
sampling 45% (146608769) splicing reads. 513189 splicing junctions. 226506 known splicing junctions. 286683 novel splicing junctions.
sampling 50% (162898632) splicing reads. 527493 splicing junctions. 227725 known splicing junctions. 299768 novel splicing junctions.
sampling 55% (179188495) splicing reads. 540085 splicing junctions. 228779 known splicing junctions. 311306 novel splicing junctions.
sampling 60% (195478359) splicing reads. 551208 splicing junctions. 229627 known splicing junctions. 321581 novel splicing junctions.
sampling 65% (211768222) splicing reads. 561012 splicing junctions. 230426 known splicing junctions. 330586 novel splicing junctions.
sampling 70% (228058085) splicing reads. 569522 splicing junctions. 231090 known splicing junctions. 338432 novel splicing junctions.
sampling 75% (244347948) splicing reads. 577299 splicing junctions. 231665 known splicing junctions. 345634 novel splicing junctions.
sampling 80% (260637812) splicing reads. 584003 splicing junctions. 232166 known splicing junctions. 351837 novel splicing junctions.
sampling 85% (276927675) splicing reads. 589845 splicing junctions. 232553 known splicing junctions. 357292 novel splicing junctions.
sampling 90% (293217538) splicing reads. 594629 splicing junctions. 232926 known splicing junctions. 361703 novel splicing junctions.
sampling 95% (309507401) splicing reads. 598789 splicing junctions. 233239 known splicing junctions. 365550 novel splicing junctions.
sampling 100% (325797265) splicing reads. 602113 splicing junctions. 233487 known splicing junctions. 368626 novel splicing junctions.