[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0754d2-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (899669) splicing reads. 106024 splicing junctions. 96248 known splicing junctions. 9776 novel splicing junctions.
sampling 10% (1799339) splicing reads. 124558 splicing junctions. 110075 known splicing junctions. 14483 novel splicing junctions.
sampling 15% (2699008) splicing reads. 133535 splicing junctions. 116273 known splicing junctions. 17262 novel splicing junctions.
sampling 20% (3598678) splicing reads. 138930 splicing junctions. 119676 known splicing junctions. 19254 novel splicing junctions.
sampling 25% (4498347) splicing reads. 142477 splicing junctions. 121834 known splicing junctions. 20643 novel splicing junctions.
sampling 30% (5398017) splicing reads. 145055 splicing junctions. 123366 known splicing junctions. 21689 novel splicing junctions.
sampling 35% (6297686) splicing reads. 146978 splicing junctions. 124479 known splicing junctions. 22499 novel splicing junctions.
sampling 40% (7197356) splicing reads. 148455 splicing junctions. 125324 known splicing junctions. 23131 novel splicing junctions.
sampling 45% (8097025) splicing reads. 149669 splicing junctions. 126016 known splicing junctions. 23653 novel splicing junctions.
sampling 50% (8996695) splicing reads. 150622 splicing junctions. 126537 known splicing junctions. 24085 novel splicing junctions.
sampling 55% (9896364) splicing reads. 151372 splicing junctions. 126927 known splicing junctions. 24445 novel splicing junctions.
sampling 60% (10796034) splicing reads. 151966 splicing junctions. 127239 known splicing junctions. 24727 novel splicing junctions.
sampling 65% (11695703) splicing reads. 152489 splicing junctions. 127508 known splicing junctions. 24981 novel splicing junctions.
sampling 70% (12595373) splicing reads. 152901 splicing junctions. 127725 known splicing junctions. 25176 novel splicing junctions.
sampling 75% (13495042) splicing reads. 153252 splicing junctions. 127912 known splicing junctions. 25340 novel splicing junctions.
sampling 80% (14394712) splicing reads. 153524 splicing junctions. 128057 known splicing junctions. 25467 novel splicing junctions.
sampling 85% (15294381) splicing reads. 153762 splicing junctions. 128186 known splicing junctions. 25576 novel splicing junctions.
sampling 90% (16194051) splicing reads. 153929 splicing junctions. 128270 known splicing junctions. 25659 novel splicing junctions.
sampling 95% (17093720) splicing reads. 154031 splicing junctions. 128319 known splicing junctions. 25712 novel splicing junctions.
sampling 100% (17993390) splicing reads. 154087 splicing junctions. 128347 known splicing junctions. 25740 novel splicing junctions.