Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a6/4a7c7d4e85efc225ebabaf5fe2f65c/aih-tih-sc-377071-R1_A23YTGFLT4_1_1.fastp.fastq.gz Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/47/c5643fa7f81cd0ecf8198fa965c624/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/47/c5643fa7f81cd0ecf8198fa965c624/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a6/4a7c7d4e85efc225ebabaf5fe2f65c/aih-tih-sc-377071-R1_A23YTGFLT4_1_2.fastp.fastq.gz ==> STAGING COMPLETE (6 inputs) STAR --genomeDir ref_genome.fa.star.idx --readFilesIn input1/aih-tih-sc-377071-R1_A23YTGFLT4_1_1.fastp.fastq.gz input2/aih-tih-sc-377071-R1_A23YTGFLT4_1_2.fastp.fastq.gz --runThreadN 24 --outFileNamePrefix aih-tih-sc-377071-R1_A23YTGFLT4_1. --sjdbGTFfile ref_annot.gtf --outSAMattrRGline ID:aih-tih-sc-377071-R1_A23YTGFLT4_1 SM:aih-tih-sc-377071-R1_A23YTGFLT4_1 --twopassMode None --chimSegmentMin 12 --chimJunctionOverhangMin 5 --chimMultimapNmax 50 --chimScoreJunctionNonGTAG -4 --chimScoreSeparation 1 --alignIntronMax 140000 --outReadsUnmapped None --readFilesCommand zcat --outSAMtype BAM SortedByCoordinate --outSAMstrandField intronMotif --outSAMunmapped Within --chimOutJunctionFormat 1 --alignSJDBoverhangMin 10 --alignMatesGapMax 100000 --alignSJstitchMismatchNmax 5 -1 5 5 --chimMultimapScoreRange 3 --chimNonchimScoreDropMin 10 --peOverlapNbasesMin 12 --peOverlapMMp 0.1 --alignInsertionFlush Right --alignSplicedMateMapLmin 30 --alignSplicedMateMapLminOverLmate 0.5 --quantMode GeneCounts --runRNGseed 1 --chimOutType Junctions WithinBAM --limitBAMsortRAM 38654705664 STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source Jun 08 07:41:57 ..... started STAR run Jun 08 07:41:57 ..... loading genome Jun 08 07:42:22 ..... processing annotations GTF Jun 08 07:42:35 ..... inserting junctions into the genome indices Jun 08 07:44:08 ..... started mapping Jun 08 08:55:56 ..... finished mapping Jun 08 08:56:01 ..... started sorting BAM Jun 08 09:01:22 ..... finished successfully