Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1b/69553138798f83f8e80b390f11a8e4/aih-tih-sc-3377ac-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d7/3c8d62dc6d65d71fe07b3391a2e13d/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e8/fbb3aa0460c083853c9b897b8e44b5/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f7/22c1c312a741a59ca8bd3cbc27edb6/aih-tih-sc-3377ac-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e8/fbb3aa0460c083853c9b897b8e44b5/.command.run
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16422855) splicing reads. 242265 splicing junctions. 180967 known splicing junctions. 61298 novel splicing junctions.
sampling 10% (32845711) splicing reads. 288913 splicing junctions. 192899 known splicing junctions. 96014 novel splicing junctions.
sampling 15% (49268566) splicing reads. 320125 splicing junctions. 199203 known splicing junctions. 120922 novel splicing junctions.
sampling 20% (65691422) splicing reads. 344005 splicing junctions. 203393 known splicing junctions. 140612 novel splicing junctions.
sampling 25% (82114277) splicing reads. 362594 splicing junctions. 206379 known splicing junctions. 156215 novel splicing junctions.
sampling 30% (98537133) splicing reads. 377887 splicing junctions. 208587 known splicing junctions. 169300 novel splicing junctions.
sampling 35% (114959988) splicing reads. 390411 splicing junctions. 210285 known splicing junctions. 180126 novel splicing junctions.
sampling 40% (131382844) splicing reads. 400841 splicing junctions. 211658 known splicing junctions. 189183 novel splicing junctions.
sampling 45% (147805699) splicing reads. 409711 splicing junctions. 212767 known splicing junctions. 196944 novel splicing junctions.
sampling 50% (164228555) splicing reads. 417260 splicing junctions. 213698 known splicing junctions. 203562 novel splicing junctions.
sampling 55% (180651410) splicing reads. 423758 splicing junctions. 214466 known splicing junctions. 209292 novel splicing junctions.
sampling 60% (197074266) splicing reads. 429452 splicing junctions. 215142 known splicing junctions. 214310 novel splicing junctions.
sampling 65% (213497121) splicing reads. 434224 splicing junctions. 215725 known splicing junctions. 218499 novel splicing junctions.
sampling 70% (229919977) splicing reads. 438429 splicing junctions. 216204 known splicing junctions. 222225 novel splicing junctions.
sampling 75% (246342832) splicing reads. 441998 splicing junctions. 216618 known splicing junctions. 225380 novel splicing junctions.
sampling 80% (262765688) splicing reads. 444862 splicing junctions. 216910 known splicing junctions. 227952 novel splicing junctions.
sampling 85% (279188543) splicing reads. 447244 splicing junctions. 217174 known splicing junctions. 230070 novel splicing junctions.
sampling 90% (295611399) splicing reads. 449107 splicing junctions. 217400 known splicing junctions. 231707 novel splicing junctions.
sampling 95% (312034254) splicing reads. 450545 splicing junctions. 217546 known splicing junctions. 232999 novel splicing junctions.
sampling 100% (328457110) splicing reads. 451511 splicing junctions. 217666 known splicing junctions. 233845 novel splicing junctions.
null device 
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