+ mkdir aih-tih-sc-1274d4-R1_A23YTGFLT4_1 + all_qc -bam aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-1274d4-R1_A23YTGFLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai 111178it [00:00, 1111645.89it/s] 225353it [00:00, 1129315.93it/s] 340285it [00:00, 1138413.12it/s] 456959it [00:00, 1149566.95it/s] 574380it [00:00, 1158424.52it/s] 690829it [00:00, 1160460.73it/s] 806876it [00:00, 1142105.28it/s] 921145it [00:00, 1127885.30it/s] 1033996it [00:00, 1124338.01it/s] 1146470it [00:01, 1119000.68it/s] 1259974it [00:01, 1123821.28it/s] 1372381it [00:01, 1120652.67it/s] 1484463it [00:01, 1115807.09it/s] 1600629it [00:01, 1129514.14it/s] 1716190it [00:01, 1137309.15it/s] 1833373it [00:01, 1147634.59it/s] 1948155it [00:01, 1141631.99it/s] 2062571it [00:01, 1142361.48it/s] 2180865it [00:01, 1154477.01it/s] 2296327it [00:02, 1149975.35it/s] 2411367it [00:02, 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[00:05, 1082868.29it/s] 5688467it [00:05, 1099107.28it/s] 5805067it [00:05, 1119047.46it/s] 5922114it [00:05, 1134365.69it/s] 6039262it [00:05, 1145451.04it/s] 6157192it [00:05, 1155564.41it/s] 6273284it [00:05, 1157074.29it/s] 6389048it [00:06, 1153166.12it/s] 6509005it [00:06, 1166940.70it/s] 6629410it [00:06, 1178028.45it/s] 6749213it [00:06, 1184000.22it/s] 6867633it [00:06, 1177701.92it/s] 6985424it [00:06, 1174652.33it/s] 7102905it [00:06, 1171781.91it/s] 7221628it [00:06, 1176372.17it/s] 7342028it [00:06, 1184606.50it/s] 7460552it [00:06, 1184776.11it/s] 7579037it [00:07, 1184678.47it/s] 7697510it [00:07, 1182009.41it/s] 7815716it [00:07, 1180028.79it/s] 7933723it [00:07, 1178050.38it/s] 8051731it [00:07, 1178639.14it/s] 8170589it [00:07, 1181591.29it/s] 8288751it [00:07, 1181329.44it/s] 8406886it [00:07, 1175767.49it/s] 8524470it [00:07, 1171365.19it/s] 8641613it [00:07, 1163222.97it/s] 8757949it [00:08, 1149732.17it/s] 8872956it [00:08, 1136158.29it/s] 8991038it [00:08, 1149294.09it/s] 9106762it [00:08, 1151574.18it/s] 9225387it [00:08, 1161795.76it/s] 9343376it [00:08, 1167170.19it/s] 9461489it [00:08, 1171320.25it/s] 9580499it [00:08, 1176915.52it/s] 9698209it [00:08, 1176625.12it/s] 9817677it [00:08, 1182012.01it/s] 9936200it [00:09, 1182956.25it/s] 10054503it [00:09, 1182617.83it/s] 10172770it [00:09, 1181349.69it/s] 10291435it [00:09, 1182905.87it/s] 10409729it [00:09, 1180317.87it/s] 10528301it [00:09, 1181915.86it/s] 10648930it [00:09, 1189196.65it/s] 10767853it [00:09, 1184247.12it/s] 10886406it [00:09, 1184611.48it/s] 11004872it [00:09, 1181243.76it/s] 11123001it [00:10, 1175924.85it/s] 11240601it [00:10, 1173466.84it/s] 11357953it [00:10, 1169280.56it/s] 11475776it [00:10, 1171928.53it/s] 11592974it [00:10, 1171683.11it/s] 11710336it [00:10, 1172166.49it/s] 11827706it [00:10, 1172603.19it/s] 11944969it [00:10, 1171056.23it/s] 12063583it [00:10, 1175448.00it/s] 12181130it [00:10, 1168463.84it/s] 12300701it [00:11, 1176575.01it/s] 12418370it [00:11, 1166177.74it/s] 12535013it [00:11, 1165483.81it/s] 12651992it [00:11, 1166747.53it/s] 12769190it [00:11, 1168288.65it/s] 12887560it [00:11, 1172880.48it/s] 13004857it [00:11, 1170540.37it/s] 13124129it [00:11, 1177150.92it/s] 13242449it [00:11, 1178936.02it/s] 13360348it [00:11, 1173456.58it/s] 13480316it [00:12, 1181271.41it/s] 13600563it [00:12, 1187591.66it/s] 13719331it [00:12, 1186845.06it/s] 13838022it [00:12, 1185384.00it/s] 13956565it [00:12, 1184317.96it/s] 14075000it [00:12, 1179658.74it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai 14192972it [00:12, 1177362.31it/s] 14310713it [00:12, 1163730.52it/s] 14427119it [00:12, 1147490.40it/s] 14543848it [00:12, 1153306.87it/s] 14660614it [00:13, 1157512.87it/s] 14777481it [00:13, 1160723.96it/s] 14896086it [00:13, 1168252.35it/s] 15014513it [00:13, 1173016.88it/s] 15131836it [00:13, 1170063.24it/s] 15249569it [00:13, 1172210.75it/s] 15366802it [00:13, 1171691.00it/s] 15483980it [00:13, 1165864.96it/s] 15600578it [00:13, 1165360.59it/s] 15717122it [00:13, 1162026.66it/s] 15833331it [00:14, 1158771.12it/s] 15949214it [00:14, 1154695.35it/s] 16064689it [00:14, 1149350.03it/s] 16181239it [00:14, 1154136.49it/s] 16297552it [00:14, 1156675.65it/s] 16415038it [00:14, 1162089.71it/s] 16532567it [00:14, 1165952.96it/s] 16651071it [00:14, 1171647.39it/s] 16769674it [00:14, 1175935.60it/s] 16887272it [00:14, 1171528.37it/s] 17006857it [00:15, 1178780.85it/s] 17124742it [00:15, 1178368.83it/s] 17244582it [00:15, 1184345.17it/s] 17363021it [00:15, 1183402.90it/s] 17483029it [00:15, 1188258.83it/s] 17602651it [00:15, 1190625.25it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai 17721716it [00:15, 1179858.33it/s] 17839725it [00:15, 1173802.85it/s] 17958310it [00:15, 1177359.13it/s] 18076351it [00:15, 1178244.97it/s] 18194698it [00:16, 1179786.91it/s] 18312686it [00:16, 1177131.33it/s] 18430589it [00:16, 1177679.88it/s] 18548769it [00:16, 1178890.36it/s] 18578339it [00:16, 1131633.36it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md.bai + merge_qc -folders aih-tih-sc-1274d4-R1_A23YTGFLT4_1/aih-tih-sc-1274d4-R1_A23YTGFLT4_1.md -output aih-tih-sc-1274d4-R1_A23YTGFLT4_1/aih-tih-sc-1274d4-R1_A23YTGFLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-1274d4-R1_A23YTGFLT4_1/aih-tih-sc-1274d4-R1_A23YTGFLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-1274d4-R1_A23YTGFLT4_1/aih-tih-sc-1274d4-R1_A23YTGFLT4_1_full_basic_stats.tsv