+ mkdir aih-tih-sc-3b436b-R1_A23YTGFLT4_1 + all_qc -bam aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-3b436b-R1_A23YTGFLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai 0it [00:00, ?it/s] 144942it [00:00, 1449331.54it/s] 303038it [00:00, 1526713.99it/s] 466963it [00:00, 1578065.12it/s] 630349it [00:00, 1600045.55it/s] 797258it [00:00, 1624913.76it/s] 964835it [00:00, 1642066.38it/s] 1131305it [00:00, 1649438.76it/s] 1297264it [00:00, 1652633.09it/s] 1464613it [00:00, 1659126.17it/s] 1631350it [00:01, 1661644.66it/s] 1797515it [00:01, 1659314.94it/s] 1963448it [00:01, 1642468.59it/s] 2127732it [00:01, 1630949.11it/s] 2290863it [00:01, 1625316.97it/s] 2453419it [00:01, 1618661.41it/s] 2618593it [00:01, 1628429.27it/s] 2786528it [00:01, 1643607.43it/s] 2954364it [00:01, 1653975.64it/s] 3119835it [00:01, 1654169.96it/s] 3285268it [00:02, 1641286.80it/s] 3451631it [00:02, 1647915.61it/s] 3616450it [00:02, 1601507.26it/s] 3776886it [00:02, 1443342.20it/s] 3924169it [00:02, 1386840.86it/s] 4072547it [00:02, 1413184.65it/s] 4238119it [00:02, 1481033.97it/s] 4406386it [00:02, 1538593.27it/s] 4573864it [00:02, 1578012.91it/s] 4732861it [00:02, 1523391.00it/s] 4886359it [00:03, 1462698.75it/s] 5046383it [00:03, 1501468.46it/s] 5212268it [00:03, 1546648.89it/s] 5379704it [00:03, 1583773.62it/s] 5547338it [00:03, 1610921.76it/s] 5712456it [00:03, 1622780.83it/s] 5876825it [00:03, 1628962.43it/s] 6045891it [00:03, 1647301.95it/s] 6210853it [00:03, 1641989.10it/s] 6375216it [00:04, 1636496.22it/s] 6542175it [00:04, 1646325.90it/s] 6709972it [00:04, 1655731.09it/s] 6875970it [00:04, 1656964.08it/s] 6948076it [00:04, 1597117.43it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md.bai + merge_qc -folders aih-tih-sc-3b436b-R1_A23YTGFLT4_1/aih-tih-sc-3b436b-R1_A23YTGFLT4_1.md -output aih-tih-sc-3b436b-R1_A23YTGFLT4_1/aih-tih-sc-3b436b-R1_A23YTGFLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-3b436b-R1_A23YTGFLT4_1/aih-tih-sc-3b436b-R1_A23YTGFLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-3b436b-R1_A23YTGFLT4_1/aih-tih-sc-3b436b-R1_A23YTGFLT4_1_full_basic_stats.tsv