[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-24ecfe-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (1034451) splicing reads. 108010 splicing junctions. 101682 known splicing junctions. 6328 novel splicing junctions.
sampling 10% (2068903) splicing reads. 125620 splicing junctions. 116226 known splicing junctions. 9394 novel splicing junctions.
sampling 15% (3103355) splicing reads. 134012 splicing junctions. 122693 known splicing junctions. 11319 novel splicing junctions.
sampling 20% (4137807) splicing reads. 138900 splicing junctions. 126248 known splicing junctions. 12652 novel splicing junctions.
sampling 25% (5172258) splicing reads. 142153 splicing junctions. 128533 known splicing junctions. 13620 novel splicing junctions.
sampling 30% (6206710) splicing reads. 144543 splicing junctions. 130142 known splicing junctions. 14401 novel splicing junctions.
sampling 35% (7241162) splicing reads. 146371 splicing junctions. 131351 known splicing junctions. 15020 novel splicing junctions.
sampling 40% (8275614) splicing reads. 147761 splicing junctions. 132221 known splicing junctions. 15540 novel splicing junctions.
sampling 45% (9310065) splicing reads. 148877 splicing junctions. 132927 known splicing junctions. 15950 novel splicing junctions.
sampling 50% (10344517) splicing reads. 149802 splicing junctions. 133482 known splicing junctions. 16320 novel splicing junctions.
sampling 55% (11378969) splicing reads. 150504 splicing junctions. 133905 known splicing junctions. 16599 novel splicing junctions.
sampling 60% (12413421) splicing reads. 151129 splicing junctions. 134260 known splicing junctions. 16869 novel splicing junctions.
sampling 65% (13447872) splicing reads. 151616 splicing junctions. 134516 known splicing junctions. 17100 novel splicing junctions.
sampling 70% (14482324) splicing reads. 152059 splicing junctions. 134771 known splicing junctions. 17288 novel splicing junctions.
sampling 75% (15516776) splicing reads. 152393 splicing junctions. 134967 known splicing junctions. 17426 novel splicing junctions.
sampling 80% (16551228) splicing reads. 152650 splicing junctions. 135105 known splicing junctions. 17545 novel splicing junctions.
sampling 85% (17585679) splicing reads. 152848 splicing junctions. 135216 known splicing junctions. 17632 novel splicing junctions.
sampling 90% (18620131) splicing reads. 153012 splicing junctions. 135307 known splicing junctions. 17705 novel splicing junctions.
sampling 95% (19654583) splicing reads. 153094 splicing junctions. 135350 known splicing junctions. 17744 novel splicing junctions.
sampling 100% (20689035) splicing reads. 153142 splicing junctions. 135374 known splicing junctions. 17768 novel splicing junctions.