[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16935083) splicing reads. 275050 splicing junctions. 190237 known splicing junctions. 84813 novel splicing junctions.
sampling 10% (33870167) splicing reads. 339155 splicing junctions. 203539 known splicing junctions. 135616 novel splicing junctions.
sampling 15% (50805251) splicing reads. 385235 splicing junctions. 210688 known splicing junctions. 174547 novel splicing junctions.
sampling 20% (67740335) splicing reads. 422205 splicing junctions. 215522 known splicing junctions. 206683 novel splicing junctions.
sampling 25% (84675419) splicing reads. 453058 splicing junctions. 219165 known splicing junctions. 233893 novel splicing junctions.
sampling 30% (101610503) splicing reads. 479433 splicing junctions. 221946 known splicing junctions. 257487 novel splicing junctions.
sampling 35% (118545586) splicing reads. 502096 splicing junctions. 224093 known splicing junctions. 278003 novel splicing junctions.
sampling 40% (135480670) splicing reads. 522337 splicing junctions. 225820 known splicing junctions. 296517 novel splicing junctions.
sampling 45% (152415754) splicing reads. 540366 splicing junctions. 227359 known splicing junctions. 313007 novel splicing junctions.
sampling 50% (169350838) splicing reads. 556308 splicing junctions. 228631 known splicing junctions. 327677 novel splicing junctions.
sampling 55% (186285922) splicing reads. 570565 splicing junctions. 229701 known splicing junctions. 340864 novel splicing junctions.
sampling 60% (203221006) splicing reads. 583308 splicing junctions. 230624 known splicing junctions. 352684 novel splicing junctions.
sampling 65% (220156090) splicing reads. 594621 splicing junctions. 231411 known splicing junctions. 363210 novel splicing junctions.
sampling 70% (237091173) splicing reads. 604784 splicing junctions. 232127 known splicing junctions. 372657 novel splicing junctions.
sampling 75% (254026257) splicing reads. 613851 splicing junctions. 232731 known splicing junctions. 381120 novel splicing junctions.
sampling 80% (270961341) splicing reads. 621998 splicing junctions. 233300 known splicing junctions. 388698 novel splicing junctions.
sampling 85% (287896425) splicing reads. 628989 splicing junctions. 233771 known splicing junctions. 395218 novel splicing junctions.
sampling 90% (304831509) splicing reads. 635148 splicing junctions. 234148 known splicing junctions. 401000 novel splicing junctions.
sampling 95% (321766593) splicing reads. 640434 splicing junctions. 234492 known splicing junctions. 405942 novel splicing junctions.
sampling 100% (338701677) splicing reads. 644959 splicing junctions. 234799 known splicing junctions. 410160 novel splicing junctions.