[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (13136763) splicing reads. 253986 splicing junctions. 180430 known splicing junctions. 73556 novel splicing junctions.
sampling 10% (26273526) splicing reads. 311400 splicing junctions. 193015 known splicing junctions. 118385 novel splicing junctions.
sampling 15% (39410289) splicing reads. 351932 splicing junctions. 199732 known splicing junctions. 152200 novel splicing junctions.
sampling 20% (52547052) splicing reads. 383423 splicing junctions. 204225 known splicing junctions. 179198 novel splicing junctions.
sampling 25% (65683816) splicing reads. 408728 splicing junctions. 207515 known splicing junctions. 201213 novel splicing junctions.
sampling 30% (78820579) splicing reads. 429645 splicing junctions. 209969 known splicing junctions. 219676 novel splicing junctions.
sampling 35% (91957342) splicing reads. 447228 splicing junctions. 211860 known splicing junctions. 235368 novel splicing junctions.
sampling 40% (105094105) splicing reads. 462276 splicing junctions. 213364 known splicing junctions. 248912 novel splicing junctions.
sampling 45% (118230868) splicing reads. 475277 splicing junctions. 214665 known splicing junctions. 260612 novel splicing junctions.
sampling 50% (131367632) splicing reads. 486728 splicing junctions. 215745 known splicing junctions. 270983 novel splicing junctions.
sampling 55% (144504395) splicing reads. 496610 splicing junctions. 216659 known splicing junctions. 279951 novel splicing junctions.
sampling 60% (157641158) splicing reads. 504895 splicing junctions. 217370 known splicing junctions. 287525 novel splicing junctions.
sampling 65% (170777921) splicing reads. 512095 splicing junctions. 218013 known splicing junctions. 294082 novel splicing junctions.
sampling 70% (183914684) splicing reads. 518312 splicing junctions. 218552 known splicing junctions. 299760 novel splicing junctions.
sampling 75% (197051448) splicing reads. 523548 splicing junctions. 218999 known splicing junctions. 304549 novel splicing junctions.
sampling 80% (210188211) splicing reads. 527960 splicing junctions. 219406 known splicing junctions. 308554 novel splicing junctions.
sampling 85% (223324974) splicing reads. 531657 splicing junctions. 219725 known splicing junctions. 311932 novel splicing junctions.
sampling 90% (236461737) splicing reads. 534571 splicing junctions. 219961 known splicing junctions. 314610 novel splicing junctions.
sampling 95% (249598500) splicing reads. 536744 splicing junctions. 220125 known splicing junctions. 316619 novel splicing junctions.
sampling 100% (262735264) splicing reads. 538179 splicing junctions. 220250 known splicing junctions. 317929 novel splicing junctions.