+ mkdir aih-tih-sc-01c779-R1_A23YTGFLT4_1 + all_qc -bam aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-01c779-R1_A23YTGFLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai 0it [00:00, ?it/s] 155478it [00:00, 1554570.22it/s] 317412it [00:00, 1592644.30it/s] 484447it [00:00, 1628051.27it/s] 647253it [00:00, 1616286.91it/s] 815284it [00:00, 1639240.29it/s] 987189it [00:00, 1666280.09it/s] 1157525it [00:00, 1678337.10it/s] 1329391it [00:00, 1691143.18it/s] 1500174it [00:00, 1696344.94it/s] 1672314it [00:01, 1704062.56it/s] 1846007it [00:01, 1713981.05it/s] 2017411it [00:01, 1707717.29it/s] 2190438it [00:01, 1714516.87it/s] 2361898it [00:01, 1703309.26it/s] 2533662it [00:01, 1707591.50it/s] 2705110it [00:01, 1709545.58it/s] 2876812it [00:01, 1711600.98it/s] 3047982it [00:01, 1702470.38it/s] 3218245it [00:01, 1695552.23it/s] 3391414it [00:02, 1706218.84it/s] 3570954it [00:02, 1732846.01it/s] 3744263it [00:02, 1731746.16it/s] 3918240it [00:02, 1734135.46it/s] 4091666it [00:02, 1733253.75it/s] 4265000it [00:02, 1724620.82it/s] 4438448it [00:02, 1727550.66it/s] 4614719it [00:02, 1738048.67it/s] 4788536it [00:02, 1728234.99it/s] 4961379it [00:02, 1678262.99it/s] 5132802it [00:03, 1688757.23it/s] 5305950it [00:03, 1701318.90it/s] 5479848it [00:03, 1712319.11it/s] 5651226it [00:03, 1707747.71it/s] 5822103it [00:03, 1704856.65it/s] 5992659it [00:03, 1702988.56it/s] 6165426it [00:03, 1710213.65it/s] 6336486it [00:03, 1706404.57it/s] 6507260it [00:03, 1706769.87it/s] 6677956it [00:03, 1702888.42it/s] 6848642it [00:04, 1703416.78it/s] 7018994it [00:04, 1703114.13it/s] 7194945it [00:04, 1719977.78it/s] 7366952it [00:04, 1719194.34it/s] 7540031it [00:04, 1722566.50it/s] 7714520it [00:04, 1729242.31it/s] 7887449it [00:04, 1728909.87it/s] 8061968it [00:04, 1733773.88it/s] 8235348it [00:04, 1732266.35it/s] 8410137it [00:04, 1736923.50it/s] 8584033it [00:05, 1737385.52it/s] 8757887it [00:05, 1737712.30it/s] 8932594it [00:05, 1740505.36it/s] 9106646it [00:05, 1725933.67it/s] 9280987it [00:05, 1731132.96it/s] 9454514it [00:05, 1732354.48it/s] 9627766it [00:05, 1728280.64it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai 9800607it [00:05, 1724533.73it/s] 9973070it [00:05, 1724441.38it/s] 10145521it [00:05, 1709395.26it/s] 10317860it [00:06, 1713475.85it/s] 10490729it [00:06, 1717999.49it/s] 10665666it [00:06, 1727195.68it/s] 10843035it [00:06, 1741087.58it/s] 11017500it [00:06, 1742052.64it/s] 11191717it [00:06, 1740887.23it/s] 11365814it [00:06, 1734602.07it/s] 11539875it [00:06, 1736279.66it/s] 11713511it [00:06, 1733041.39it/s] 11886822it [00:06, 1732397.84it/s] 12060066it [00:07, 1730896.84it/s] 12236055it [00:07, 1739449.67it/s] 12410004it [00:07, 1736987.84it/s] 12583706it [00:07, 1731748.87it/s] 12756886it [00:07, 1730743.38it/s] 12933245it [00:07, 1740507.86it/s] 13107301it [00:07, 1727172.98it/s] 13280043it [00:07, 1694330.31it/s] 13379510it [00:07, 1713871.10it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.md.bai + merge_qc -folders aih-tih-sc-01c779-R1_A23YTGFLT4_1/aih-tih-sc-01c779-R1_A23YTGFLT4_1.md -output aih-tih-sc-01c779-R1_A23YTGFLT4_1/aih-tih-sc-01c779-R1_A23YTGFLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-01c779-R1_A23YTGFLT4_1/aih-tih-sc-01c779-R1_A23YTGFLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-01c779-R1_A23YTGFLT4_1/aih-tih-sc-01c779-R1_A23YTGFLT4_1_full_basic_stats.tsv