[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3270ab-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	325902503
Known Splicing Events:	322739047
Partial Novel Splicing Events:	1528951
Novel Splicing Events:	1534328
Filtered Splicing Events:	100177

Total splicing  Junctions:	602324
Known Splicing Junctions:	289818
Partial Novel Splicing Junctions:	174534
Novel Splicing Junctions:	137972

===================================================================
Create BED file ...
Create Interact file ...