+ mkdir aih-tih-sc-377071-R1_A23YTGFLT4_1 + all_qc -bam aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-377071-R1_A23YTGFLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai 154323it [00:00, 1543135.81it/s] 318691it [00:00, 1602242.91it/s] 485736it [00:00, 1633339.61it/s] 656405it [00:00, 1662272.16it/s] 826233it [00:00, 1675233.98it/s] 996859it [00:00, 1685758.50it/s] 1165435it [00:00, 1683532.73it/s] 1336445it [00:00, 1691958.84it/s] 1507341it [00:00, 1697102.62it/s] 1677907it [00:01, 1699724.85it/s] 1847881it [00:01, 1699244.11it/s] 2017807it [00:01, 1696930.47it/s] 2187502it [00:01, 1692556.25it/s] 2359013it [00:01, 1699331.24it/s] 2529909it [00:01, 1702209.13it/s] 2700134it [00:01, 1694957.65it/s] 2873332it [00:01, 1705985.03it/s] 3049637it [00:01, 1723065.80it/s] 3221956it [00:01, 1716012.49it/s] 3393571it [00:02, 1695235.48it/s] 3563153it [00:02, 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1700131.97it/s] 8670595it [00:05, 1702969.99it/s] 8840948it [00:05, 1703124.84it/s] 9011264it [00:05, 1702982.55it/s] 9182051it [00:05, 1704327.48it/s] 9352486it [00:05, 1637836.81it/s] 9516823it [00:05, 1611112.03it/s] 9678355it [00:05, 1575771.88it/s] 9836302it [00:05, 1542324.84it/s] 10005575it [00:05, 1585516.40it/s] 10167344it [00:06, 1594632.80it/s] 10332251it [00:06, 1610596.49it/s] 10500801it [00:06, 1632577.86it/s] 10668681it [00:06, 1646279.12it/s] 10835764it [00:06, 1653572.29it/s] 11004764it [00:06, 1664395.23it/s] 11175316it [00:06, 1676666.03it/s] 11343940it [00:06, 1679512.07it/s] 11516905it [00:06, 1694508.28it/s] 11687934it [00:06, 1699218.98it/s] 11859151it [00:07, 1703022.14it/s] 12029472it [00:07, 1699993.76it/s] 12199486it [00:07, 1694000.02it/s] 12368900it [00:07, 1688365.92it/s] 12539024it [00:07, 1692195.10it/s] 12708254it [00:07, 1687856.22it/s] 12877048it [00:07, 1682655.37it/s] 13045321it [00:07, 1676795.75it/s] 13213008it [00:07, 1671444.95it/s] 13380159it [00:07, 1658150.99it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai 13545994it [00:08, 1646147.60it/s] 13710631it [00:08, 1645258.44it/s] 13875172it [00:08, 1636054.16it/s] 14038792it [00:08, 1623038.14it/s] 14209754it [00:08, 1648664.42it/s] 14380214it [00:08, 1665277.35it/s] 14551202it [00:08, 1678552.81it/s] 14723762it [00:08, 1692592.88it/s] 14893058it [00:08, 1690480.46it/s] 15062400it [00:08, 1691343.19it/s] 15231553it [00:09, 1687872.43it/s] 15354968it [00:09, 1678295.16it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-377071-R1_A23YTGFLT4_1.md.bai + merge_qc -folders aih-tih-sc-377071-R1_A23YTGFLT4_1/aih-tih-sc-377071-R1_A23YTGFLT4_1.md -output aih-tih-sc-377071-R1_A23YTGFLT4_1/aih-tih-sc-377071-R1_A23YTGFLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-377071-R1_A23YTGFLT4_1/aih-tih-sc-377071-R1_A23YTGFLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-377071-R1_A23YTGFLT4_1/aih-tih-sc-377071-R1_A23YTGFLT4_1_full_basic_stats.tsv