[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-eecf9f-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (21247931) splicing reads. 230206 splicing junctions. 170388 known splicing junctions. 59818 novel splicing junctions.
sampling 10% (42495863) splicing reads. 278180 splicing junctions. 184922 known splicing junctions. 93258 novel splicing junctions.
sampling 15% (63743795) splicing reads. 310232 splicing junctions. 192264 known splicing junctions. 117968 novel splicing junctions.
sampling 20% (84991726) splicing reads. 334268 splicing junctions. 196833 known splicing junctions. 137435 novel splicing junctions.
sampling 25% (106239658) splicing reads. 353530 splicing junctions. 200172 known splicing junctions. 153358 novel splicing junctions.
sampling 30% (127487590) splicing reads. 369301 splicing junctions. 202658 known splicing junctions. 166643 novel splicing junctions.
sampling 35% (148735521) splicing reads. 382317 splicing junctions. 204596 known splicing junctions. 177721 novel splicing junctions.
sampling 40% (169983453) splicing reads. 393384 splicing junctions. 206168 known splicing junctions. 187216 novel splicing junctions.
sampling 45% (191231385) splicing reads. 402956 splicing junctions. 207478 known splicing junctions. 195478 novel splicing junctions.
sampling 50% (212479317) splicing reads. 411220 splicing junctions. 208542 known splicing junctions. 202678 novel splicing junctions.
sampling 55% (233727248) splicing reads. 418371 splicing junctions. 209442 known splicing junctions. 208929 novel splicing junctions.
sampling 60% (254975180) splicing reads. 424486 splicing junctions. 210209 known splicing junctions. 214277 novel splicing junctions.
sampling 65% (276223112) splicing reads. 429769 splicing junctions. 210850 known splicing junctions. 218919 novel splicing junctions.
sampling 70% (297471043) splicing reads. 434394 splicing junctions. 211412 known splicing junctions. 222982 novel splicing junctions.
sampling 75% (318718975) splicing reads. 438310 splicing junctions. 211850 known splicing junctions. 226460 novel splicing junctions.
sampling 80% (339966907) splicing reads. 441634 splicing junctions. 212214 known splicing junctions. 229420 novel splicing junctions.
sampling 85% (361214838) splicing reads. 444506 splicing junctions. 212556 known splicing junctions. 231950 novel splicing junctions.
sampling 90% (382462770) splicing reads. 446799 splicing junctions. 212791 known splicing junctions. 234008 novel splicing junctions.
sampling 95% (403710702) splicing reads. 448570 splicing junctions. 212986 known splicing junctions. 235584 novel splicing junctions.
sampling 100% (424958634) splicing reads. 449983 splicing junctions. 213139 known splicing junctions. 236844 novel splicing junctions.