[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3abdf4-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (10298920) splicing reads. 238030 splicing junctions. 180067 known splicing junctions. 57963 novel splicing junctions.
sampling 10% (20597840) splicing reads. 281442 splicing junctions. 192409 known splicing junctions. 89033 novel splicing junctions.
sampling 15% (30896760) splicing reads. 310136 splicing junctions. 198946 known splicing junctions. 111190 novel splicing junctions.
sampling 20% (41195680) splicing reads. 331108 splicing junctions. 203022 known splicing junctions. 128086 novel splicing junctions.
sampling 25% (51494600) splicing reads. 347164 splicing junctions. 206002 known splicing junctions. 141162 novel splicing junctions.
sampling 30% (61793520) splicing reads. 360117 splicing junctions. 208077 known splicing junctions. 152040 novel splicing junctions.
sampling 35% (72092441) splicing reads. 370714 splicing junctions. 209729 known splicing junctions. 160985 novel splicing junctions.
sampling 40% (82391361) splicing reads. 379580 splicing junctions. 211048 known splicing junctions. 168532 novel splicing junctions.
sampling 45% (92690281) splicing reads. 386914 splicing junctions. 212101 known splicing junctions. 174813 novel splicing junctions.
sampling 50% (102989201) splicing reads. 393031 splicing junctions. 212995 known splicing junctions. 180036 novel splicing junctions.
sampling 55% (113288121) splicing reads. 398408 splicing junctions. 213736 known splicing junctions. 184672 novel splicing junctions.
sampling 60% (123587041) splicing reads. 403208 splicing junctions. 214352 known splicing junctions. 188856 novel splicing junctions.
sampling 65% (133885961) splicing reads. 407116 splicing junctions. 214889 known splicing junctions. 192227 novel splicing junctions.
sampling 70% (144184882) splicing reads. 410411 splicing junctions. 215330 known splicing junctions. 195081 novel splicing junctions.
sampling 75% (154483802) splicing reads. 413241 splicing junctions. 215711 known splicing junctions. 197530 novel splicing junctions.
sampling 80% (164782722) splicing reads. 415587 splicing junctions. 216004 known splicing junctions. 199583 novel splicing junctions.
sampling 85% (175081642) splicing reads. 417389 splicing junctions. 216237 known splicing junctions. 201152 novel splicing junctions.
sampling 90% (185380562) splicing reads. 418966 splicing junctions. 216463 known splicing junctions. 202503 novel splicing junctions.
sampling 95% (195679482) splicing reads. 420122 splicing junctions. 216606 known splicing junctions. 203516 novel splicing junctions.
sampling 100% (205978403) splicing reads. 420903 splicing junctions. 216729 known splicing junctions. 204174 novel splicing junctions.