+ mkdir aih-tih-sc-318819-R1_A23YTGFLT4_1 + all_qc -bam aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-318819-R1_A23YTGFLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai 130276it [00:00, 1302618.38it/s] 285740it [00:00, 1450823.95it/s] 430823it [00:00, 1429766.55it/s] 594585it [00:00, 1511064.57it/s] 753959it [00:00, 1540499.24it/s] 918025it [00:00, 1574400.28it/s] 1083414it [00:00, 1600261.58it/s] 1245741it [00:00, 1607387.31it/s] 1408886it [00:00, 1614879.79it/s] 1574971it [00:01, 1629037.58it/s] 1745940it [00:01, 1653686.56it/s] 1911323it [00:01, 1595743.40it/s] 2080511it [00:01, 1624114.33it/s] 2244927it [00:01, 1630034.12it/s] 2410596it [00:01, 1637942.15it/s] 2579974it [00:01, 1654577.79it/s] 2750005it [00:01, 1668219.79it/s] 2916945it [00:01, 1622790.84it/s] 3090322it [00:01, 1655347.50it/s] 3256168it [00:02, 1629754.52it/s] 3419416it [00:02, 1581129.23it/s] 3577947it [00:02, 1545578.79it/s] 3740237it [00:02, 1567666.67it/s] 3911067it [00:02, 1608386.12it/s] 4077060it [00:02, 1623493.16it/s] 4247501it [00:02, 1647323.14it/s] 4421435it [00:02, 1674611.01it/s] 4589093it [00:02, 1638928.99it/s] 4753272it [00:02, 1616339.67it/s] 4915135it [00:03, 1564027.54it/s] 5074337it [00:03, 1572055.63it/s] 5231873it [00:03, 1538135.68it/s] 5385997it [00:03, 1530053.93it/s] 5539202it [00:03, 1450673.94it/s] 5710350it [00:03, 1524407.62it/s] 5881688it [00:03, 1578750.44it/s] 6054205it [00:03, 1621378.67it/s] 6228804it [00:03, 1657883.26it/s] 6395944it [00:04, 1661725.54it/s] 6569726it [00:04, 1684287.63it/s] 6742815it [00:04, 1698136.94it/s] 6917654it [00:04, 1713104.19it/s] 7093357it [00:04, 1726204.77it/s] 7269012it [00:04, 1735251.36it/s] 7444112it [00:04, 1739937.03it/s] 7618172it [00:04, 1735060.58it/s] 7791727it [00:04, 1673136.93it/s] 7963797it [00:04, 1686889.66it/s] 8134457it [00:05, 1692647.34it/s] 8305768it [00:05, 1698643.01it/s] 8475838it [00:05, 1598436.20it/s] 8636974it [00:05, 1451836.28it/s] 8785242it [00:05, 1425353.57it/s] 8938264it [00:05, 1453996.09it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai 9111023it [00:05, 1530809.01it/s] 9285542it [00:05, 1592134.52it/s] 9452583it [00:05, 1614829.99it/s] 9621236it [00:05, 1635764.15it/s] 9792557it [00:06, 1658595.49it/s] 9964042it [00:06, 1675251.56it/s] 10138446it [00:06, 1695690.67it/s] 10312989it [00:06, 1710498.41it/s] 10486006it [00:06, 1716312.17it/s] 10660753it [00:06, 1725604.25it/s] 10833437it [00:06, 1646411.97it/s] 10998922it [00:06, 1617708.82it/s] 11166740it [00:06, 1635148.52it/s] 11330757it [00:07, 1630610.84it/s] 11494161it [00:07, 1589036.81it/s] 11653466it [00:07, 1562080.06it/s] 11812212it [00:07, 1569277.57it/s] 11890005it [00:07, 1613898.52it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.md.bai + merge_qc -folders aih-tih-sc-318819-R1_A23YTGFLT4_1/aih-tih-sc-318819-R1_A23YTGFLT4_1.md -output aih-tih-sc-318819-R1_A23YTGFLT4_1/aih-tih-sc-318819-R1_A23YTGFLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-318819-R1_A23YTGFLT4_1/aih-tih-sc-318819-R1_A23YTGFLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-318819-R1_A23YTGFLT4_1/aih-tih-sc-318819-R1_A23YTGFLT4_1_full_basic_stats.tsv