+ mkdir aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1 + all_qc -bam aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai 0it [00:00, ?it/s] 105049it [00:00, 1050015.35it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai 217017it [00:00, 1090825.41it/s] 326100it [00:00, 1089292.54it/s] 440886it [00:00, 1112367.39it/s] 554481it [00:00, 1120842.41it/s] 667645it [00:00, 1124490.52it/s] 784050it [00:00, 1137341.90it/s] 899879it [00:00, 1143986.01it/s] 1014279it [00:00, 1103568.68it/s] 1124928it [00:01, 1086739.32it/s] 1233823it [00:01, 1078979.66it/s] 1342118it [00:01, 1080135.86it/s] 1456880it [00:01, 1100200.44it/s] 1571147it [00:01, 1112844.73it/s] 1685221it [00:01, 1121161.45it/s] 1798225it [00:01, 1123799.33it/s] 1910657it [00:01, 1112714.08it/s] 2022613it [00:01, 1114732.69it/s] 2134128it [00:01, 1101761.75it/s] 2244360it [00:02, 1085961.35it/s] 2353027it [00:02, 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[00:08, 1068271.98it/s] 9043866it [00:08, 1066757.79it/s] 9150551it [00:08, 1050516.92it/s] 9266713it [00:08, 1083352.34it/s] 9383330it [00:08, 1107930.19it/s] 9499475it [00:08, 1123850.27it/s] 9618177it [00:08, 1142686.67it/s] 9735067it [00:08, 1150357.79it/s] 9850147it [00:08, 1147117.07it/s] 9964891it [00:09, 1146001.35it/s] 10082710it [00:09, 1155607.00it/s] 10199140it [00:09, 1158190.75it/s] 10314973it [00:09, 1139088.91it/s] 10428960it [00:09, 1116683.27it/s] 10540756it [00:09, 1086700.23it/s] 10649634it [00:09, 1079848.87it/s] 10757752it [00:09, 1072598.45it/s] 10865095it [00:09, 1059129.09it/s] 10971072it [00:10, 1050711.37it/s] 11081093it [00:10, 1065175.03it/s] 11199476it [00:10, 1100132.09it/s] 11316680it [00:10, 1121352.96it/s] 11433999it [00:10, 1136748.14it/s] 11547758it [00:10, 1133145.02it/s] 11661132it [00:10, 1117786.92it/s] 11772991it [00:10, 1095804.32it/s] 11888932it [00:10, 1114408.26it/s] 12002716it [00:10, 1121294.85it/s] 12114951it [00:11, 1102636.44it/s] 12226891it [00:11, 1107543.24it/s] 12337743it [00:11, 1084864.66it/s] 12446373it [00:11, 1083083.71it/s] 12554779it [00:11, 1068389.62it/s] 12661706it [00:11, 1060934.63it/s] 12689956it [00:11, 1095653.75it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md.bai + merge_qc -folders aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1/aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1.md -output aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1/aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1/aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1/aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1_full_basic_stats.tsv