Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 244443028 total bases: 36910897228 Q20 bases: 36003476866(97.5416%) Q30 bases: 34468871607(93.384%) Read2 before filtering: total reads: 244443028 total bases: 36910897228 Q20 bases: 36133192211(97.893%) Q30 bases: 34459005403(93.3573%) Read1 after filtering: total reads: 241243052 total bases: 27606760561 Q20 bases: 27488366773(99.5711%) Q30 bases: 26943407653(97.5971%) Read2 after filtering: total reads: 241243052 total bases: 27644050889 Q20 bases: 27469330007(99.368%) Q30 bases: 26827007622(97.0444%) Filtering result: reads passed filter: 482486104 reads failed due to low quality: 5972896 reads failed due to too many N: 16746 reads failed due to too short: 410310 reads with adapter trimmed: 372268163 bases trimmed due to adapters: 17743619191 Duplication rate: 33.5659% Insert size peak (evaluated by paired-end reads): 98 JSON report: aih-tih-sc-ecc0bc-R1_A23YTGFLT4_1.fastp.json HTML report: aih-tih-sc-ecc0bc-R1_A23YTGFLT4_1.fastp.html fastp --in1 aih-tih-sc-ecc0bc-R1_A23YTGFLT4_1_1.fastq.gz --in2 aih-tih-sc-ecc0bc-R1_A23YTGFLT4_1_2.fastq.gz --out1 aih-tih-sc-ecc0bc-R1_A23YTGFLT4_1_1.fastp.fastq.gz --out2 aih-tih-sc-ecc0bc-R1_A23YTGFLT4_1_2.fastp.fastq.gz --json aih-tih-sc-ecc0bc-R1_A23YTGFLT4_1.fastp.json --html aih-tih-sc-ecc0bc-R1_A23YTGFLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 651 seconds