[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3282fd-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	222767920
Known Splicing Events:	220489398
Partial Novel Splicing Events:	1233476
Novel Splicing Events:	931523
Filtered Splicing Events:	113523

Total splicing  Junctions:	474341
Known Splicing Junctions:	253708
Partial Novel Splicing Junctions:	117365
Novel Splicing Junctions:	103268

===================================================================
Create BED file ...
Create Interact file ...