[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-318819-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (10715638) splicing reads. 235483 splicing junctions. 175283 known splicing junctions. 60200 novel splicing junctions.
sampling 10% (21431276) splicing reads. 278396 splicing junctions. 186325 known splicing junctions. 92071 novel splicing junctions.
sampling 15% (32146915) splicing reads. 305744 splicing junctions. 191860 known splicing junctions. 113884 novel splicing junctions.
sampling 20% (42862553) splicing reads. 324891 splicing junctions. 195283 known splicing junctions. 129608 novel splicing junctions.
sampling 25% (53578191) splicing reads. 339434 splicing junctions. 197731 known splicing junctions. 141703 novel splicing junctions.
sampling 30% (64293830) splicing reads. 350747 splicing junctions. 199484 known splicing junctions. 151263 novel splicing junctions.
sampling 35% (75009468) splicing reads. 359800 splicing junctions. 200854 known splicing junctions. 158946 novel splicing junctions.
sampling 40% (85725106) splicing reads. 367159 splicing junctions. 201853 known splicing junctions. 165306 novel splicing junctions.
sampling 45% (96440745) splicing reads. 373350 splicing junctions. 202685 known splicing junctions. 170665 novel splicing junctions.
sampling 50% (107156383) splicing reads. 378414 splicing junctions. 203336 known splicing junctions. 175078 novel splicing junctions.
sampling 55% (117872021) splicing reads. 382723 splicing junctions. 203851 known splicing junctions. 178872 novel splicing junctions.
sampling 60% (128587660) splicing reads. 386429 splicing junctions. 204296 known splicing junctions. 182133 novel splicing junctions.
sampling 65% (139303298) splicing reads. 389498 splicing junctions. 204685 known splicing junctions. 184813 novel splicing junctions.
sampling 70% (150018936) splicing reads. 392038 splicing junctions. 204994 known splicing junctions. 187044 novel splicing junctions.
sampling 75% (160734575) splicing reads. 394033 splicing junctions. 205213 known splicing junctions. 188820 novel splicing junctions.
sampling 80% (171450213) splicing reads. 395704 splicing junctions. 205420 known splicing junctions. 190284 novel splicing junctions.
sampling 85% (182165851) splicing reads. 397012 splicing junctions. 205584 known splicing junctions. 191428 novel splicing junctions.
sampling 90% (192881490) splicing reads. 398002 splicing junctions. 205703 known splicing junctions. 192299 novel splicing junctions.
sampling 95% (203597128) splicing reads. 398655 splicing junctions. 205790 known splicing junctions. 192865 novel splicing junctions.
sampling 100% (214312767) splicing reads. 399047 splicing junctions. 205838 known splicing junctions. 193209 novel splicing junctions.