[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16293190) splicing reads. 269150 splicing junctions. 191367 known splicing junctions. 77783 novel splicing junctions.
sampling 10% (32586380) splicing reads. 329036 splicing junctions. 204397 known splicing junctions. 124639 novel splicing junctions.
sampling 15% (48879570) splicing reads. 372089 splicing junctions. 211516 known splicing junctions. 160573 novel splicing junctions.
sampling 20% (65172761) splicing reads. 406567 splicing junctions. 216282 known splicing junctions. 190285 novel splicing junctions.
sampling 25% (81465951) splicing reads. 435396 splicing junctions. 219805 known splicing junctions. 215591 novel splicing junctions.
sampling 30% (97759141) splicing reads. 460059 splicing junctions. 222580 known splicing junctions. 237479 novel splicing junctions.
sampling 35% (114052332) splicing reads. 481472 splicing junctions. 224716 known splicing junctions. 256756 novel splicing junctions.
sampling 40% (130345522) splicing reads. 500207 splicing junctions. 226521 known splicing junctions. 273686 novel splicing junctions.
sampling 45% (146638712) splicing reads. 516481 splicing junctions. 227971 known splicing junctions. 288510 novel splicing junctions.
sampling 50% (162931903) splicing reads. 531235 splicing junctions. 229293 known splicing junctions. 301942 novel splicing junctions.
sampling 55% (179225093) splicing reads. 544272 splicing junctions. 230376 known splicing junctions. 313896 novel splicing junctions.
sampling 60% (195518283) splicing reads. 556129 splicing junctions. 231304 known splicing junctions. 324825 novel splicing junctions.
sampling 65% (211811473) splicing reads. 566644 splicing junctions. 232120 known splicing junctions. 334524 novel splicing junctions.
sampling 70% (228104664) splicing reads. 575994 splicing junctions. 232802 known splicing junctions. 343192 novel splicing junctions.
sampling 75% (244397854) splicing reads. 584277 splicing junctions. 233440 known splicing junctions. 350837 novel splicing junctions.
sampling 80% (260691044) splicing reads. 591603 splicing junctions. 233970 known splicing junctions. 357633 novel splicing junctions.
sampling 85% (276984235) splicing reads. 598071 splicing junctions. 234427 known splicing junctions. 363644 novel splicing junctions.
sampling 90% (293277425) splicing reads. 603726 splicing junctions. 234804 known splicing junctions. 368922 novel splicing junctions.
sampling 95% (309570615) splicing reads. 608570 splicing junctions. 235165 known splicing junctions. 373405 novel splicing junctions.
sampling 100% (325863806) splicing reads. 612685 splicing junctions. 235458 known splicing junctions. 377227 novel splicing junctions.