[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-92d360-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (10671024) splicing reads. 233482 splicing junctions. 177871 known splicing junctions. 55611 novel splicing junctions.
sampling 10% (21342049) splicing reads. 278935 splicing junctions. 189992 known splicing junctions. 88943 novel splicing junctions.
sampling 15% (32013074) splicing reads. 309908 splicing junctions. 196307 known splicing junctions. 113601 novel splicing junctions.
sampling 20% (42684099) splicing reads. 333073 splicing junctions. 200416 known splicing junctions. 132657 novel splicing junctions.
sampling 25% (53355124) splicing reads. 351265 splicing junctions. 203250 known splicing junctions. 148015 novel splicing junctions.
sampling 30% (64026148) splicing reads. 366237 splicing junctions. 205458 known splicing junctions. 160779 novel splicing junctions.
sampling 35% (74697173) splicing reads. 378497 splicing junctions. 207120 known splicing junctions. 171377 novel splicing junctions.
sampling 40% (85368198) splicing reads. 388923 splicing junctions. 208480 known splicing junctions. 180443 novel splicing junctions.
sampling 45% (96039223) splicing reads. 397803 splicing junctions. 209569 known splicing junctions. 188234 novel splicing junctions.
sampling 50% (106710248) splicing reads. 405299 splicing junctions. 210470 known splicing junctions. 194829 novel splicing junctions.
sampling 55% (117381272) splicing reads. 411664 splicing junctions. 211252 known splicing junctions. 200412 novel splicing junctions.
sampling 60% (128052297) splicing reads. 417151 splicing junctions. 211874 known splicing junctions. 205277 novel splicing junctions.
sampling 65% (138723322) splicing reads. 421844 splicing junctions. 212408 known splicing junctions. 209436 novel splicing junctions.
sampling 70% (149394347) splicing reads. 425777 splicing junctions. 212869 known splicing junctions. 212908 novel splicing junctions.
sampling 75% (160065372) splicing reads. 429047 splicing junctions. 213245 known splicing junctions. 215802 novel splicing junctions.
sampling 80% (170736396) splicing reads. 431750 splicing junctions. 213530 known splicing junctions. 218220 novel splicing junctions.
sampling 85% (181407421) splicing reads. 433988 splicing junctions. 213790 known splicing junctions. 220198 novel splicing junctions.
sampling 90% (192078446) splicing reads. 435685 splicing junctions. 213959 known splicing junctions. 221726 novel splicing junctions.
sampling 95% (202749471) splicing reads. 436924 splicing junctions. 214107 known splicing junctions. 222817 novel splicing junctions.
sampling 100% (213420496) splicing reads. 437736 splicing junctions. 214210 known splicing junctions. 223526 novel splicing junctions.