+ mkdir aih-tih-sc-3377ac-R1_A23YTGFLT4_1 + all_qc -bam aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-3377ac-R1_A23YTGFLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai 0it [00:00, ?it/s] 142391it [00:00, 1423795.94it/s] 304992it [00:00, 1542513.03it/s] 474651it [00:00, 1612828.23it/s] 640909it [00:00, 1632438.15it/s] 810650it [00:00, 1655835.37it/s] 980820it [00:00, 1671395.26it/s] 1149442it [00:00, 1676212.06it/s] 1320692it [00:00, 1687645.29it/s] 1492314it [00:00, 1696548.90it/s] 1661969it [00:01, 1691042.36it/s] 1831084it [00:01, 1690780.31it/s] 2000165it [00:01, 1670981.88it/s] 2171258it [00:01, 1682942.71it/s] 2347568it [00:01, 1706967.08it/s] 2518840it [00:01, 1708635.00it/s] 2689738it [00:01, 1708574.00it/s] 2860620it [00:01, 1546060.61it/s] 3032425it [00:01, 1594094.89it/s] 3195248it [00:01, 1603645.11it/s] 3357354it [00:02, 1574692.08it/s] 3528008it [00:02, 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1695005.32it/s] 8596327it [00:05, 1692301.34it/s] 8765578it [00:05, 1680635.39it/s] 8933672it [00:05, 1672529.48it/s] 9105328it [00:05, 1685561.06it/s] 9277147it [00:05, 1695269.38it/s] 9449751it [00:05, 1704312.04it/s] 9620204it [00:05, 1697011.22it/s] 9795581it [00:05, 1713927.62it/s] 9968910it [00:05, 1719590.22it/s] 10140887it [00:06, 1714802.11it/s] 10312382it [00:06, 1694824.10it/s] 10481921it [00:06, 1683498.15it/s] 10650316it [00:06, 1658335.88it/s] 10816238it [00:06, 1640958.86it/s] 10981784it [00:06, 1645191.07it/s] 11151465it [00:06, 1660372.94it/s] 11320567it [00:06, 1669450.70it/s] 11490740it [00:06, 1679043.94it/s] 11663420it [00:07, 1693277.20it/s] 11832787it [00:07, 1692024.03it/s] 12005689it [00:07, 1703048.89it/s] 12177001it [00:07, 1706039.76it/s] 12347622it [00:07, 1701674.99it/s] 12517803it [00:07, 1638651.07it/s] 12682165it [00:07, 1625526.21it/s] 12834070it [00:07, 1659699.49it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md.bai + merge_qc -folders aih-tih-sc-3377ac-R1_A23YTGFLT4_1/aih-tih-sc-3377ac-R1_A23YTGFLT4_1.md -output aih-tih-sc-3377ac-R1_A23YTGFLT4_1/aih-tih-sc-3377ac-R1_A23YTGFLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-3377ac-R1_A23YTGFLT4_1/aih-tih-sc-3377ac-R1_A23YTGFLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-3377ac-R1_A23YTGFLT4_1/aih-tih-sc-3377ac-R1_A23YTGFLT4_1_full_basic_stats.tsv