[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-184b05-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14827826) splicing reads. 266324 splicing junctions. 187600 known splicing junctions. 78724 novel splicing junctions.
sampling 10% (29655652) splicing reads. 324104 splicing junctions. 200373 known splicing junctions. 123731 novel splicing junctions.
sampling 15% (44483478) splicing reads. 364197 splicing junctions. 206937 known splicing junctions. 157260 novel splicing junctions.
sampling 20% (59311305) splicing reads. 394977 splicing junctions. 211217 known splicing junctions. 183760 novel splicing junctions.
sampling 25% (74139131) splicing reads. 420079 splicing junctions. 214376 known splicing junctions. 205703 novel splicing junctions.
sampling 30% (88966957) splicing reads. 440965 splicing junctions. 216736 known splicing junctions. 224229 novel splicing junctions.
sampling 35% (103794784) splicing reads. 458830 splicing junctions. 218673 known splicing junctions. 240157 novel splicing junctions.
sampling 40% (118622610) splicing reads. 473770 splicing junctions. 220182 known splicing junctions. 253588 novel splicing junctions.
sampling 45% (133450436) splicing reads. 486906 splicing junctions. 221476 known splicing junctions. 265430 novel splicing junctions.
sampling 50% (148278263) splicing reads. 497959 splicing junctions. 222492 known splicing junctions. 275467 novel splicing junctions.
sampling 55% (163106089) splicing reads. 507816 splicing junctions. 223391 known splicing junctions. 284425 novel splicing junctions.
sampling 60% (177933915) splicing reads. 516417 splicing junctions. 224148 known splicing junctions. 292269 novel splicing junctions.
sampling 65% (192761741) splicing reads. 523680 splicing junctions. 224812 known splicing junctions. 298868 novel splicing junctions.
sampling 70% (207589568) splicing reads. 530127 splicing junctions. 225381 known splicing junctions. 304746 novel splicing junctions.
sampling 75% (222417394) splicing reads. 535736 splicing junctions. 225833 known splicing junctions. 309903 novel splicing junctions.
sampling 80% (237245220) splicing reads. 540483 splicing junctions. 226192 known splicing junctions. 314291 novel splicing junctions.
sampling 85% (252073047) splicing reads. 544530 splicing junctions. 226534 known splicing junctions. 317996 novel splicing junctions.
sampling 90% (266900873) splicing reads. 547828 splicing junctions. 226805 known splicing junctions. 321023 novel splicing junctions.
sampling 95% (281728699) splicing reads. 550570 splicing junctions. 227047 known splicing junctions. 323523 novel splicing junctions.
sampling 100% (296556526) splicing reads. 552608 splicing junctions. 227207 known splicing junctions. 325401 novel splicing junctions.