[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-ec2fd6-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14487828) splicing reads. 261723 splicing junctions. 186902 known splicing junctions. 74821 novel splicing junctions.
sampling 10% (28975656) splicing reads. 321845 splicing junctions. 199868 known splicing junctions. 121977 novel splicing junctions.
sampling 15% (43463484) splicing reads. 365224 splicing junctions. 206836 known splicing junctions. 158388 novel splicing junctions.
sampling 20% (57951313) splicing reads. 399587 splicing junctions. 211356 known splicing junctions. 188231 novel splicing junctions.
sampling 25% (72439141) splicing reads. 427828 splicing junctions. 214668 known splicing junctions. 213160 novel splicing junctions.
sampling 30% (86926969) splicing reads. 451955 splicing junctions. 217284 known splicing junctions. 234671 novel splicing junctions.
sampling 35% (101414797) splicing reads. 472281 splicing junctions. 219311 known splicing junctions. 252970 novel splicing junctions.
sampling 40% (115902626) splicing reads. 489986 splicing junctions. 220977 known splicing junctions. 269009 novel splicing junctions.
sampling 45% (130390454) splicing reads. 505304 splicing junctions. 222345 known splicing junctions. 282959 novel splicing junctions.
sampling 50% (144878282) splicing reads. 518776 splicing junctions. 223499 known splicing junctions. 295277 novel splicing junctions.
sampling 55% (159366110) splicing reads. 530607 splicing junctions. 224539 known splicing junctions. 306068 novel splicing junctions.
sampling 60% (173853939) splicing reads. 540762 splicing junctions. 225378 known splicing junctions. 315384 novel splicing junctions.
sampling 65% (188341767) splicing reads. 549811 splicing junctions. 226052 known splicing junctions. 323759 novel splicing junctions.
sampling 70% (202829595) splicing reads. 557766 splicing junctions. 226698 known splicing junctions. 331068 novel splicing junctions.
sampling 75% (217317423) splicing reads. 564455 splicing junctions. 227172 known splicing junctions. 337283 novel splicing junctions.
sampling 80% (231805252) splicing reads. 570158 splicing junctions. 227576 known splicing junctions. 342582 novel splicing junctions.
sampling 85% (246293080) splicing reads. 575107 splicing junctions. 227933 known splicing junctions. 347174 novel splicing junctions.
sampling 90% (260780908) splicing reads. 579025 splicing junctions. 228254 known splicing junctions. 350771 novel splicing junctions.
sampling 95% (275268736) splicing reads. 582132 splicing junctions. 228483 known splicing junctions. 353649 novel splicing junctions.
sampling 100% (289756565) splicing reads. 584413 splicing junctions. 228684 known splicing junctions. 355729 novel splicing junctions.