Done.
-parsing GTF file: /tmp/nxf.20rcjYX9p4/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.gtf
* Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.20rcjYX9p4/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.20rcjYX9p4/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.20rcjYX9p4/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.20rcjYX9p4/fi_workdir/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.20rcjYX9p4/fi_workdir/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c'
* Running CMD: mv Aligned.sortedByCoord.out.bam aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam
* Running CMD: samtools index aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam
11:38:29 : INFO : Done.
-parsing GTF file: /tmp/nxf.20rcjYX9p4/fi_workdir/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.gtf
-parsing /tmp/nxf.20rcjYX9p4/fi_workdir/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam
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[1170000] -done parsing /tmp/nxf.20rcjYX9p4/fi_workdir/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam. Extracting junction info.
junction read elimination tally: $VAR1 = {
'excessive soft clipping' => 8791,
'exons hit < 2' => 6,
'low complexity anchor region' => 9,
'num_hits: 6 != num_counted_on_fusion_contigs 4 ' => 200,
'small anchor length' => 3,
'per_id < 96' => 4291,
'num genes matched < 2' => 80,
'num_hits: 4 != num_counted_on_fusion_contigs 3 ' => 12,
'seq-similar region overlap' => 108,
'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 119,
' ** passed ** ' => 2510,
'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 37,
'num_hits: 4 != num_counted_on_fusion_contigs 2 ' => 42,
'num_hits: 3 != num_counted_on_fusion_contigs 2 ' => 30
};
-writing fusion junction support info.
-outputting the spanning read info: /tmp/nxf.20rcjYX9p4/fi_workdir/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info.
- counting read alignments among fusion contigs.
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[102000] -fusion SPANNING read extraction for scaff: ABAT--GSG1L
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[14000] -fusion SPANNING read extraction for scaff: AKAP10--KCNU1
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[13000] -fusion SPANNING read extraction for scaff: AKAP10--AC090453.1
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[17000] -fusion SPANNING read extraction for scaff: AP3M2--NRG1
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[155000] -fusion SPANNING read extraction for scaff: CCDC189--MROH1
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[21000] -fusion SPANNING read extraction for scaff: CMTM8--RN7SL120P
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[21000] -fusion SPANNING read extraction for scaff: CMTM8--CNTN6
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[40000] -fusion SPANNING read extraction for scaff: CUL1--RBMS3
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[66000] -fusion SPANNING read extraction for scaff: EZH1--TBC1D16
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[64000] -fusion SPANNING read extraction for scaff: GBE1--FOXP1
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[31000] -fusion SPANNING read extraction for scaff: LRMDA--ADK
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[75000] -fusion SPANNING read extraction for scaff: N4BP2L2--LITAFD
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[9000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC1
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[9000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC2
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[10000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHA13
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[9000] -fusion SPANNING read extraction for scaff: PCDHA3--PCDHAC1
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[10000] -fusion SPANNING read extraction for scaff: PCDHA3--PCDHA13
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[70000] -fusion SPANNING read extraction for scaff: STX16--NPEPL1
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[48000] -fusion SPANNING read extraction for scaff: SUCLG2--CNTN3
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[34000] -fusion SPANNING read extraction for scaff: SUCLG2--NIPA2P2
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[32000] -fusion SPANNING read extraction for scaff: SUCLG2-AS1--PTPRG
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[52000] -fusion SPANNING read extraction for scaff: TCF4--HNRNPA1P57
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[8000] -fusion SPANNING read extraction for scaff: TDRD1--HECTD2
[1000] -fusion SPANNING read extraction for scaff: TDRD1--RPS27P1
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[26000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1
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[5000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1
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[14000]
[15000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4
[1000]
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[47000]
[48000]
[49000]
[50000]
[51000]
[52000]
[53000]
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[55000]
[56000] -fusion SPANNING read extraction for scaff: XRN2--GRM7
[1000]
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[6000]
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[8000]
[9000]
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[43000]
[44000]
[45000]
[46000]
[47000]
[48000]
[49000]
[50000]
[51000]
[52000] -fusion SPANNING read extraction for scaff: ZC3HAV1--CNTNAP2
[1000]
[2000]
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[42000] -fusion SPANNING read extraction for scaff: ZNF267--SPECC1
-filtered reads reasons: $VAR1 = {
'seq similar region alignment' => 8757,
'lacks exon overlap' => 17238
};
EM: Starting log likelihood: -3895.010331
EM: Round [1] log likelihood: -3894.735160
EM: Round [2] log likelihood: -3894.612311
EM: Round [3] log likelihood: -3894.508321
EM: Round [4] log likelihood: -3894.419700
EM: Round [5] log likelihood: -3894.343822
EM: Round [6] log likelihood: -3894.278601
EM: Round [7] log likelihood: -3894.222356
EM: Round [8] log likelihood: -3894.173718
EM: Round [9] log likelihood: -3894.131560
EM: Round [10] log likelihood: -3894.094944
EM: Round [11] log likelihood: -3894.063088
EM: Round [12] log likelihood: -3894.035331
EM: Round [13] log likelihood: -3894.011116
EM: Round [14] log likelihood: -3893.989968
EM: Round [15] log likelihood: -3893.971479
EM: Round [16] log likelihood: -3893.955303
EM: Round [17] log likelihood: -3893.941140
EM: Round [18] log likelihood: -3893.928732
EM: Round [19] log likelihood: -3893.917855
EM: Round [20] log likelihood: -3893.908315
EM: Round [21] log likelihood: -3893.899945
EM: Round [22] log likelihood: -3893.892599
EM: Round [23] log likelihood: -3893.886150
EM: Round [24] log likelihood: -3893.880486
EM: Round [25] log likelihood: -3893.875510
EM: Round [26] log likelihood: -3893.871138
EM: Round [27] log likelihood: -3893.867296
EM: Round [28] log likelihood: -3893.863919
EM: Round [29] log likelihood: -3893.860951
EM: Round [30] log likelihood: -3893.858341
EM: Round [31] log likelihood: -3893.856046
EM: Round [32] log likelihood: -3893.854028
EM: Round [33] log likelihood: -3893.852254
EM: Round [34] log likelihood: -3893.850693
EM: Round [35] log likelihood: -3893.849320
EM: Round [36] log likelihood: -3893.848112
EM: Round [37] log likelihood: -3893.847050
EM: Round [38] log likelihood: -3893.846116
EM: Round [39] log likelihood: -3893.845293
EM: Round [40] log likelihood: -3893.844570
EM: Round [41] log likelihood: -3893.843934
EM: Round [42] log likelihood: -3893.843374
EM: Round [43] log likelihood: -3893.842881
EM: Round [44] log likelihood: -3893.842448
EM: Round [45] log likelihood: -3893.842067
EM: Round [46] log likelihood: -3893.841731
EM: Round [47] log likelihood: -3893.841436
EM: Round [48] log likelihood: -3893.841176
EM: Round [49] log likelihood: -3893.840947
EM: Round [50] log likelihood: -3893.840746
EM: Round [51] log likelihood: -3893.840569
EM: Round [52] log likelihood: -3893.840413
EM: Round [53] log likelihood: -3893.840276
EM: Round [54] log likelihood: -3893.840156
EM: Round [55] log likelihood: -3893.840050
EM: Round [56] log likelihood: -3893.839956
EM: Stopping iterations at round 56 due to insufficient improvement in likelihood.
[bam_sort_core] merging from 0 files and 8 in-memory blocks...
-extracting read coordinates from /tmp/nxf.20rcjYX9p4/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.consolidated.bam into /tmp/nxf.20rcjYX9p4/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.consolidated.bam.read_coords
CMD: touch /tmp/nxf.20rcjYX9p4/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.consolidated.bam.read_coords.ok
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.20rcjYX9p4/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.consolidated.bam.read_coords > /tmp/nxf.20rcjYX9p4/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.consolidated.bam.read_coords.sort_by_readname
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.20rcjYX9p4/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.consolidated.bam.frag_coords > /tmp/nxf.20rcjYX9p4/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.consolidated.bam.frag_coords.coord_sorted
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.20rcjYX9p4/fi_workdir/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.20rcjYX9p4/fi_workdir/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50
CMD: cp /tmp/nxf.20rcjYX9p4/fi_workdir/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.20rcjYX9p4/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter
-done, see /tmp/nxf.20rcjYX9p4/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass
EM: Starting log likelihood: -3541.392340
EM: Round [1] log likelihood: -3541.347793
EM: Round [2] log likelihood: -3541.347712
EM: Stopping iterations at round 2 due to insufficient improvement in likelihood.
-total frags in /tmp/nxf.20rcjYX9p4/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1_1.fastp.fastq.gz: 296820409
Warning - not locating file: /tmp/nxf.20rcjYX9p4/aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1.gmap_trinity_GG.fusions.gff3.bed
WARNING, no entry stored in dbm for [ENSG00000262668.2]
WARNING, no entry stored in dbm for [ENSG00000226049.3]
WARNING, no entry stored in dbm for [ENSG00000260404.3]
WARNING, no entry stored in dbm for [ENSG00000280136.2]
WARNING, no entry stored in dbm for [ENSG00000237442.3]
WARNING, no entry stored in dbm for [ENSG00000241316.8]
WARNING, no entry stored in dbm for [ENSG00000241316.8]
ls: cannot access 'IGV_inputs': No such file or directory