[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-31d31f-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	138841252
Known Splicing Events:	137157335
Partial Novel Splicing Events:	927476
Novel Splicing Events:	696710
Filtered Splicing Events:	59731

Total splicing  Junctions:	285852
Known Splicing Junctions:	207465
Partial Novel Splicing Junctions:	50424
Novel Splicing Junctions:	27963

===================================================================
Create BED file ...
Create Interact file ...