Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d7/3c8d62dc6d65d71fe07b3391a2e13d/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/76/86e22d14260876a367f2d02d536add/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/76/86e22d14260876a367f2d02d536add/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/af/a085fbd289e2e08075078f1660e007/aih-tih-sc-13d441-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/fb/68a6e2a23ce8557c7d28f14943b6ab/aih-tih-sc-13d441-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-13d441-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (13444256) splicing reads. 268121 splicing junctions. 190653 known splicing junctions. 77468 novel splicing junctions.
sampling 10% (26888513) splicing reads. 327798 splicing junctions. 203518 known splicing junctions. 124280 novel splicing junctions.
sampling 15% (40332769) splicing reads. 369998 splicing junctions. 210491 known splicing junctions. 159507 novel splicing junctions.
sampling 20% (53777026) splicing reads. 403178 splicing junctions. 215013 known splicing junctions. 188165 novel splicing junctions.
sampling 25% (67221283) splicing reads. 430617 splicing junctions. 218248 known splicing junctions. 212369 novel splicing junctions.
sampling 30% (80665539) splicing reads. 453312 splicing junctions. 220761 known splicing junctions. 232551 novel splicing junctions.
sampling 35% (94109796) splicing reads. 472844 splicing junctions. 222774 known splicing junctions. 250070 novel splicing junctions.
sampling 40% (107554053) splicing reads. 489549 splicing junctions. 224405 known splicing junctions. 265144 novel splicing junctions.
sampling 45% (120998309) splicing reads. 504333 splicing junctions. 225820 known splicing junctions. 278513 novel splicing junctions.
sampling 50% (134442566) splicing reads. 517134 splicing junctions. 226920 known splicing junctions. 290214 novel splicing junctions.
sampling 55% (147886823) splicing reads. 528487 splicing junctions. 227931 known splicing junctions. 300556 novel splicing junctions.
sampling 60% (161331079) splicing reads. 538400 splicing junctions. 228719 known splicing junctions. 309681 novel splicing junctions.
sampling 65% (174775336) splicing reads. 547029 splicing junctions. 229355 known splicing junctions. 317674 novel splicing junctions.
sampling 70% (188219593) splicing reads. 554556 splicing junctions. 229962 known splicing junctions. 324594 novel splicing junctions.
sampling 75% (201663849) splicing reads. 561325 splicing junctions. 230484 known splicing junctions. 330841 novel splicing junctions.
sampling 80% (215108106) splicing reads. 567048 splicing junctions. 230921 known splicing junctions. 336127 novel splicing junctions.
sampling 85% (228552363) splicing reads. 572042 splicing junctions. 231309 known splicing junctions. 340733 novel splicing junctions.
sampling 90% (241996619) splicing reads. 576210 splicing junctions. 231659 known splicing junctions. 344551 novel splicing junctions.
sampling 95% (255440876) splicing reads. 579554 splicing junctions. 231930 known splicing junctions. 347624 novel splicing junctions.
sampling 100% (268885133) splicing reads. 582152 splicing junctions. 232125 known splicing junctions. 350027 novel splicing junctions.
null device 
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