+ mkdir aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1 + all_qc -bam aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai 147281it [00:00, 1472765.76it/s] 310215it [00:00, 1564843.85it/s] 473062it [00:00, 1593878.06it/s] 640015it [00:00, 1623680.55it/s] 806204it [00:00, 1637442.34it/s] 972418it [00:00, 1645823.84it/s] 1138758it [00:00, 1651520.06it/s] 1306666it [00:00, 1660280.14it/s] 1476524it [00:00, 1672238.41it/s] 1643748it [00:01, 1671727.53it/s] 1811444it [00:01, 1673309.52it/s] 1978776it [00:01, 1656738.40it/s] 2144487it [00:01, 1641196.39it/s] 2313231it [00:01, 1654988.45it/s] 2479685it [00:01, 1657803.42it/s] 2646921it [00:01, 1662147.50it/s] 2821825it [00:01, 1688159.15it/s] 2991056it [00:01, 1689198.27it/s] 3159998it [00:01, 1677974.52it/s] 3327826it [00:02, 1657433.97it/s] 3496495it [00:02, 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1666836.13it/s] 8542914it [00:05, 1679537.80it/s] 8712299it [00:05, 1683702.94it/s] 8880710it [00:05, 1674189.56it/s] 9048713it [00:05, 1675837.47it/s] 9216325it [00:05, 1674497.21it/s] 9383794it [00:05, 1671278.60it/s] 9553236it [00:05, 1678168.23it/s] 9722780it [00:05, 1683272.45it/s] 9892076it [00:05, 1686154.30it/s] 10063414it [00:06, 1694294.39it/s] 10232850it [00:06, 1691603.28it/s] 10406687it [00:06, 1705592.77it/s] 10577253it [00:06, 1701901.69it/s] 10747449it [00:06, 1701094.25it/s] 10917693it [00:06, 1701481.86it/s] 11087844it [00:06, 1698579.12it/s] 11257705it [00:06, 1660315.20it/s] 11423923it [00:06, 1611376.77it/s] 11593276it [00:06, 1635060.53it/s] 11757544it [00:07, 1637278.26it/s] 11927726it [00:07, 1656306.46it/s] 12096698it [00:07, 1666201.15it/s] 12264851it [00:07, 1670749.54it/s] 12432038it [00:07, 1670199.35it/s] 12603399it [00:07, 1683148.86it/s] 12772482it [00:07, 1685430.92it/s] 12942801it [00:07, 1690719.79it/s] 13111905it [00:07, 1689900.18it/s] 13281379it [00:07, 1691333.59it/s] 13450528it [00:08, 1684772.04it/s] 13508536it [00:08, 1662623.10it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md.bai + merge_qc -folders aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1/aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1.md -output aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1/aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1/aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1/aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1_full_basic_stats.tsv