Done. -parsing GTF file: /tmp/nxf.SJUubQnIeE/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.SJUubQnIeE/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.SJUubQnIeE/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.SJUubQnIeE/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.SJUubQnIeE/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.SJUubQnIeE/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam 09:56:11 : INFO : Done. -parsing GTF file: /tmp/nxf.SJUubQnIeE/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.gtf -parsing /tmp/nxf.SJUubQnIeE/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] [140000] [150000] -done parsing /tmp/nxf.SJUubQnIeE/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'num_hits: 5 != num_counted_on_fusion_contigs 3 ' => 30, 'low complexity anchor region' => 1, 'seq-similar region overlap' => 37, 'exons hit < 2' => 3, ' ** passed ** ' => 1222, 'per_id < 96' => 567, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 11, 'excessive soft clipping' => 1108 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.SJUubQnIeE/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] -fusion SPANNING read extraction for scaff: AC004980.3--UPK3BL1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] -fusion SPANNING read extraction for scaff: PRCP--RAB30 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] -fusion SPANNING read extraction for scaff: TLK2--AL669831.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 -filtered reads reasons: $VAR1 = { 'seq similar region alignment' => 10336, 'lacks exon overlap' => 1704 }; EM: Starting log likelihood: -732.721321 EM: Round [1] log likelihood: -732.705762 EM: Round [2] log likelihood: -732.690570 EM: Round [3] log likelihood: -732.675733 EM: Round [4] log likelihood: -732.661239 EM: Round [5] log likelihood: -732.647080 EM: Round [6] log likelihood: -732.633244 EM: Round [7] log likelihood: -732.619722 EM: Round [8] log likelihood: -732.606506 EM: Round [9] log likelihood: -732.593585 EM: Round [10] log likelihood: -732.580952 EM: Round [11] log likelihood: -732.568599 EM: Round [12] log likelihood: -732.556517 EM: Round [13] log likelihood: -732.544700 EM: Round [14] log likelihood: -732.533139 EM: Round [15] log likelihood: -732.521828 EM: Round [16] log likelihood: -732.510761 EM: Round [17] log likelihood: -732.499929 EM: Round [18] log likelihood: -732.489328 EM: Round [19] log likelihood: -732.478952 EM: Round [20] log likelihood: -732.468793 EM: Round [21] log likelihood: -732.458847 EM: Round [22] log likelihood: -732.449109 EM: Round [23] log likelihood: -732.439572 EM: Round [24] log likelihood: -732.430232 EM: Round [25] log likelihood: -732.421084 EM: Round [26] log likelihood: -732.412123 EM: Round [27] log likelihood: -732.403344 EM: Round [28] log likelihood: -732.394743 EM: Round [29] log likelihood: -732.386316 EM: Round [30] log likelihood: -732.378058 EM: Round [31] log likelihood: -732.369965 EM: Round [32] log likelihood: -732.362034 EM: Round [33] log likelihood: -732.354260 EM: Round [34] log likelihood: -732.346640 EM: Round [35] log likelihood: -732.339170 EM: Round [36] log likelihood: -732.331847 EM: Round [37] log likelihood: -732.324667 EM: Round [38] log likelihood: -732.317627 EM: Round [39] log likelihood: -732.310723 EM: Round [40] log likelihood: -732.303954 EM: Round [41] log likelihood: -732.297315 EM: Round [42] log likelihood: -732.290803 EM: Round [43] log likelihood: -732.284417 EM: Round [44] log likelihood: -732.278152 EM: Round [45] log likelihood: -732.272007 EM: Round [46] log likelihood: -732.265978 EM: Round [47] log likelihood: -732.260064 EM: Round [48] log likelihood: -732.254261 EM: Round [49] log likelihood: -732.248568 EM: Round [50] log likelihood: -732.242981 EM: Round [51] log likelihood: -732.237499 EM: Round [52] log likelihood: -732.232119 EM: Round [53] log likelihood: -732.226840 EM: Round [54] log likelihood: -732.221658 EM: Round [55] log likelihood: -732.216573 EM: Round [56] log likelihood: -732.211581 EM: Round [57] log likelihood: -732.206681 EM: Round [58] log likelihood: -732.201872 EM: Round [59] log likelihood: -732.197151 EM: Round [60] log likelihood: -732.192516 EM: Round [61] log likelihood: -732.187966 EM: Round [62] log likelihood: -732.183498 EM: Round [63] log likelihood: -732.179112 EM: Round [64] log likelihood: -732.174805 EM: Round [65] log likelihood: -732.170577 EM: Round [66] log likelihood: -732.166424 EM: Round [67] log likelihood: -732.162347 EM: Round [68] log likelihood: -732.158343 EM: Round [69] log likelihood: -732.154410 EM: Round [70] log likelihood: -732.150548 EM: Round [71] log likelihood: -732.146756 EM: Round [72] log likelihood: -732.143031 EM: Round [73] log likelihood: -732.139372 EM: Round [74] log likelihood: -732.135778 EM: Round [75] log likelihood: -732.132248 EM: Round [76] log likelihood: -732.128781 EM: Round [77] log likelihood: -732.125375 EM: Round [78] log likelihood: -732.122029 EM: Round [79] log likelihood: -732.118742 EM: Round [80] log likelihood: -732.115513 EM: Round [81] log likelihood: -732.112341 EM: Round [82] log likelihood: -732.109224 EM: Round [83] log likelihood: -732.106162 EM: Round [84] log likelihood: -732.103154 EM: Round [85] log likelihood: -732.100198 EM: Round [86] log likelihood: -732.097294 EM: Round [87] log likelihood: -732.094440 EM: Round [88] log likelihood: -732.091636 EM: Round [89] log likelihood: -732.088880 EM: Round [90] log likelihood: -732.086173 EM: Round [91] log likelihood: -732.083512 EM: Round [92] log likelihood: -732.080897 EM: Round [93] log likelihood: -732.078328 EM: Round [94] log likelihood: -732.075803 EM: Round [95] log likelihood: -732.073321 EM: Round [96] log likelihood: -732.070882 EM: Round [97] log likelihood: -732.068485 EM: Round [98] log likelihood: -732.066129 EM: Round [99] log likelihood: -732.063813 EM: Round [100] log likelihood: -732.061537 EM: Round [101] log likelihood: -732.059300 EM: Round [102] log likelihood: -732.057102 EM: Round [103] log likelihood: -732.054941 EM: Round [104] log likelihood: -732.052816 EM: Round [105] log likelihood: -732.050728 EM: Round [106] log likelihood: -732.048676 EM: Round [107] log likelihood: -732.046658 EM: Round [108] log likelihood: -732.044675 EM: Round [109] log likelihood: -732.042725 EM: Round [110] log likelihood: -732.040808 EM: Round [111] log likelihood: -732.038924 EM: Round [112] log likelihood: -732.037071 EM: Round [113] log likelihood: -732.035250 EM: Round [114] log likelihood: -732.033460 EM: Round [115] log likelihood: -732.031700 EM: Round [116] log likelihood: -732.029970 EM: Round [117] log likelihood: -732.028268 EM: Round [118] log likelihood: -732.026596 EM: Round [119] log likelihood: -732.024951 EM: Round [120] log likelihood: -732.023334 EM: Round [121] log likelihood: -732.021744 EM: Round [122] log likelihood: -732.020181 EM: Round [123] log likelihood: -732.018644 EM: Round [124] log likelihood: -732.017133 EM: Round [125] log likelihood: -732.015648 EM: Round [126] log likelihood: -732.014187 EM: Round [127] log likelihood: -732.012750 EM: Round [128] log likelihood: -732.011337 EM: Round [129] log likelihood: -732.009948 EM: Round [130] log likelihood: -732.008582 EM: Round [131] log likelihood: -732.007239 EM: Round [132] log likelihood: -732.005919 EM: Round [133] log likelihood: -732.004620 EM: Round [134] log likelihood: -732.003343 EM: Round [135] log likelihood: -732.002087 EM: Round [136] log likelihood: -732.000852 EM: Round [137] log likelihood: -731.999637 EM: Round [138] log likelihood: -731.998443 EM: Round [139] log likelihood: -731.997268 EM: Round [140] log likelihood: -731.996113 EM: Round [141] log likelihood: -731.994977 EM: Round [142] log likelihood: -731.993860 EM: Round [143] log likelihood: -731.992761 EM: Round [144] log likelihood: -731.991681 EM: Round [145] log likelihood: -731.990618 EM: Round [146] log likelihood: -731.989573 EM: Round [147] log likelihood: -731.988545 EM: Round [148] log likelihood: -731.987534 EM: Round [149] log likelihood: -731.986540 EM: Round [150] log likelihood: -731.985562 EM: Round [151] log likelihood: -731.984601 EM: Round [152] log likelihood: -731.983655 EM: Round [153] log likelihood: -731.982725 EM: Round [154] log likelihood: -731.981810 EM: Round [155] log likelihood: -731.980910 EM: Round [156] log likelihood: -731.980024 EM: Round [157] log likelihood: -731.979154 EM: Round [158] log likelihood: -731.978297 EM: Round [159] log likelihood: -731.977455 EM: Round [160] log likelihood: -731.976627 EM: Round [161] log likelihood: -731.975812 EM: Round [162] log likelihood: -731.975010 EM: Round [163] log likelihood: -731.974222 EM: Round [164] log likelihood: -731.973447 EM: Round [165] log likelihood: -731.972684 EM: Round [166] log likelihood: -731.971934 EM: Round [167] log likelihood: -731.971196 EM: Round [168] log likelihood: -731.970470 EM: Round [169] log likelihood: -731.969756 EM: Round [170] log likelihood: -731.969054 EM: Round [171] log likelihood: -731.968363 EM: Round [172] log likelihood: -731.967683 EM: Round [173] log likelihood: -731.967015 EM: Round [174] log likelihood: -731.966357 EM: Round [175] log likelihood: -731.965710 EM: Round [176] log likelihood: -731.965074 EM: Round [177] log likelihood: -731.964448 EM: Round [178] log likelihood: -731.963832 EM: Round [179] log likelihood: -731.963227 EM: Round [180] log likelihood: -731.962631 EM: Round [181] log likelihood: -731.962045 EM: Round [182] log likelihood: -731.961468 EM: Round [183] log likelihood: -731.960901 EM: Round [184] log likelihood: -731.960343 EM: Round [185] log likelihood: -731.959794 EM: Round [186] log likelihood: -731.959254 EM: Round [187] log likelihood: -731.958723 EM: Round [188] log likelihood: -731.958201 EM: Round [189] log likelihood: -731.957687 EM: Round [190] log likelihood: -731.957181 EM: Round [191] log likelihood: -731.956684 EM: Round [192] log likelihood: -731.956194 EM: Round [193] log likelihood: -731.955713 EM: Round [194] log likelihood: -731.955239 EM: Round [195] log likelihood: -731.954773 EM: Round [196] log likelihood: -731.954315 EM: Round [197] log likelihood: -731.953864 EM: Round [198] log likelihood: -731.953421 EM: Round [199] log likelihood: -731.952984 EM: Round [200] log likelihood: -731.952555 EM: Round [201] log likelihood: -731.952132 EM: Round [202] log likelihood: -731.951717 EM: Round [203] log likelihood: -731.951308 EM: Round [204] log likelihood: -731.950906 EM: Round [205] log likelihood: -731.950510 EM: Round [206] log likelihood: -731.950121 EM: Round [207] log likelihood: -731.949738 EM: Round [208] log likelihood: -731.949361 EM: Round [209] log likelihood: -731.948991 EM: Round [210] log likelihood: -731.948626 EM: Round [211] log likelihood: -731.948267 EM: Round [212] log likelihood: -731.947914 EM: Round [213] log likelihood: -731.947567 EM: Round [214] log likelihood: -731.947225 EM: Round [215] log likelihood: -731.946889 EM: Round [216] log likelihood: -731.946559 EM: Round [217] log likelihood: -731.946233 EM: Round [218] log likelihood: -731.945913 EM: Round [219] log likelihood: -731.945598 EM: Round [220] log likelihood: -731.945289 EM: Round [221] log likelihood: -731.944984 EM: Round [222] log likelihood: -731.944684 EM: Round [223] log likelihood: -731.944389 EM: Round [224] log likelihood: -731.944099 EM: Round [225] log likelihood: -731.943813 EM: Round [226] log likelihood: -731.943532 EM: Round [227] log likelihood: -731.943255 EM: Round [228] log likelihood: -731.942984 EM: Round [229] log likelihood: -731.942716 EM: Round [230] log likelihood: -731.942453 EM: Round [231] log likelihood: -731.942194 EM: Round [232] log likelihood: -731.941939 EM: Round [233] log likelihood: -731.941688 EM: Round [234] log likelihood: -731.941441 EM: Round [235] log likelihood: -731.941199 EM: Round [236] log likelihood: -731.940960 EM: Round [237] log likelihood: -731.940725 EM: Round [238] log likelihood: -731.940494 EM: Round [239] log likelihood: -731.940266 EM: Round [240] log likelihood: -731.940042 EM: Round [241] log likelihood: -731.939822 EM: Round [242] log likelihood: -731.939605 EM: Round [243] log likelihood: -731.939392 EM: Round [244] log likelihood: -731.939183 EM: Round [245] log likelihood: -731.938976 EM: Round [246] log likelihood: -731.938773 EM: Round [247] log likelihood: -731.938573 EM: Round [248] log likelihood: -731.938377 EM: Round [249] log likelihood: -731.938184 EM: Round [250] log likelihood: -731.937993 EM: Round [251] log likelihood: -731.937806 EM: Round [252] log likelihood: -731.937622 EM: Round [253] log likelihood: -731.937441 EM: Round [254] log likelihood: -731.937262 EM: Round [255] log likelihood: -731.937087 EM: Round [256] log likelihood: -731.936914 EM: Round [257] log likelihood: -731.936744 EM: Round [258] log likelihood: -731.936577 EM: Round [259] log likelihood: -731.936413 EM: Round [260] log likelihood: -731.936251 EM: Round [261] log likelihood: -731.936092 EM: Round [262] log likelihood: -731.935935 EM: Round [263] log likelihood: -731.935781 EM: Round [264] log likelihood: -731.935630 EM: Round [265] log likelihood: -731.935480 EM: Round [266] log likelihood: -731.935334 EM: Round [267] log likelihood: -731.935189 EM: Round [268] log likelihood: -731.935047 EM: Round [269] log likelihood: -731.934907 EM: Round [270] log likelihood: -731.934770 EM: Round [271] log likelihood: -731.934634 EM: Round [272] log likelihood: -731.934501 EM: Round [273] log likelihood: -731.934370 EM: Round [274] log likelihood: -731.934241 EM: Round [275] log likelihood: -731.934114 EM: Round [276] log likelihood: -731.933989 EM: Round [277] log likelihood: -731.933867 EM: Round [278] log likelihood: -731.933746 EM: Round [279] log likelihood: -731.933627 EM: Round [280] log likelihood: -731.933510 EM: Round [281] log likelihood: -731.933395 EM: Round [282] log likelihood: -731.933281 EM: Round [283] log likelihood: -731.933170 EM: Round [284] log likelihood: -731.933060 EM: Round [285] log likelihood: -731.932952 EM: Round [286] log likelihood: -731.932846 EM: Round [287] log likelihood: -731.932742 EM: Round [288] log likelihood: -731.932639 EM: Round [289] log likelihood: -731.932538 EM: Round [290] log likelihood: -731.932438 EM: Stopping iterations at round 290 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.SJUubQnIeE/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.consolidated.bam into /tmp/nxf.SJUubQnIeE/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.SJUubQnIeE/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.SJUubQnIeE/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.consolidated.bam.read_coords > /tmp/nxf.SJUubQnIeE/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.SJUubQnIeE/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.consolidated.bam.frag_coords > /tmp/nxf.SJUubQnIeE/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.SJUubQnIeE/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.SJUubQnIeE/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.SJUubQnIeE/fi_workdir/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.SJUubQnIeE/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.SJUubQnIeE/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -719.400948 EM: Round [1] log likelihood: -719.400948 EM: Stopping iterations at round 1 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.SJUubQnIeE/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1_1.fastp.fastq.gz: 216439276 Warning - not locating file: /tmp/nxf.SJUubQnIeE/aih-tih-sc-a97ce1-R1_A23YTGFLT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000230305.2] WARNING, no entry stored in dbm for [ENSG00000228327.3] WARNING, no entry stored in dbm for [ENSG00000228327.3] ls: cannot access 'IGV_inputs': No such file or directory