[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	146849751
Known Splicing Events:	145510374
Partial Novel Splicing Events:	849382
Novel Splicing Events:	441590
Filtered Splicing Events:	48405

Total splicing  Junctions:	517370
Known Splicing Junctions:	277514
Partial Novel Splicing Junctions:	142518
Novel Splicing Junctions:	97338

===================================================================
Create BED file ...
Create Interact file ...