+ mkdir aih-tih-sc-01eb49-R1_A23YTGFLT4_1 + all_qc -bam aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-01eb49-R1_A23YTGFLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai 0it [00:00, ?it/s] 146151it [00:00, 1461406.87it/s] 309872it [00:00, 1564781.04it/s] 477139it [00:00, 1614014.84it/s] 645405it [00:00, 1640960.58it/s] 818601it [00:00, 1673642.72it/s] 990257it [00:00, 1688209.04it/s] 1160299it [00:00, 1692161.74it/s] 1334425it [00:00, 1707758.02it/s] 1507139it [00:00, 1713798.10it/s] 1678828it [00:01, 1714734.16it/s] 1850302it [00:01, 1700587.16it/s] 2020387it [00:01, 1680572.09it/s] 2188505it [00:01, 1677556.79it/s] 2358425it [00:01, 1684010.45it/s] 2527949it [00:01, 1687269.94it/s] 2698661it [00:01, 1693113.13it/s] 2867994it [00:01, 1688683.42it/s] 3040259it [00:01, 1698767.86it/s] 3210152it [00:01, 1694516.40it/s] 3379616it [00:02, 1674812.56it/s] 3547898it [00:02, 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1709055.24it/s] 8657443it [00:05, 1715770.07it/s] 8830912it [00:05, 1721421.23it/s] 9003058it [00:05, 1708896.49it/s] 9173971it [00:05, 1705913.89it/s] 9344578it [00:05, 1704378.31it/s] 9515027it [00:05, 1696254.08it/s] 9684666it [00:05, 1693153.12it/s] 9854581it [00:05, 1694925.46it/s] 10024081it [00:05, 1693574.07it/s] 10193443it [00:06, 1689455.16it/s] 10365603it [00:06, 1699041.54it/s] 10535514it [00:06, 1696199.06it/s] 10706286it [00:06, 1699626.63it/s] 10878601it [00:06, 1706651.25it/s] 11049359it [00:06, 1706911.64it/s] 11220054it [00:06, 1695496.81it/s] 11389623it [00:06, 1692395.97it/s] 11558876it [00:06, 1687915.56it/s] 11728027it [00:06, 1688971.65it/s] 11897657it [00:07, 1691144.12it/s] 12066778it [00:07, 1691075.05it/s] 12235890it [00:07, 1688533.53it/s] 12404747it [00:07, 1686344.16it/s] 12573384it [00:07, 1678871.52it/s] 12741279it [00:07, 1676656.74it/s] 12908958it [00:07, 1676559.72it/s] 13076888it [00:07, 1677362.96it/s] 13244627it [00:07, 1669170.92it/s] 13412494it [00:07, 1671943.90it/s] 13580333it [00:08, 1673778.37it/s] 13748556it [00:08, 1676291.27it/s] 13916191it [00:08, 1668856.10it/s] 14083087it [00:08, 1663474.98it/s] 14249444it [00:08, 1662003.29it/s] 14261288it [00:08, 1687263.93it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai + merge_qc -folders aih-tih-sc-01eb49-R1_A23YTGFLT4_1/aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md -output aih-tih-sc-01eb49-R1_A23YTGFLT4_1/aih-tih-sc-01eb49-R1_A23YTGFLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-01eb49-R1_A23YTGFLT4_1/aih-tih-sc-01eb49-R1_A23YTGFLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-01eb49-R1_A23YTGFLT4_1/aih-tih-sc-01eb49-R1_A23YTGFLT4_1_full_basic_stats.tsv