Done. -parsing GTF file: /tmp/nxf.Oz0e6MmjbU/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.Oz0e6MmjbU/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.Oz0e6MmjbU/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.Oz0e6MmjbU/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.Oz0e6MmjbU/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.Oz0e6MmjbU/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam 11:15:59 : INFO : Done. -parsing GTF file: /tmp/nxf.Oz0e6MmjbU/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.gtf -parsing /tmp/nxf.Oz0e6MmjbU/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] -done parsing /tmp/nxf.Oz0e6MmjbU/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'exons hit < 2' => 2, ' ** passed ** ' => 848, 'seq-similar region overlap' => 19, 'num genes matched < 2' => 2, 'per_id < 96' => 417, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 20, 'excessive soft clipping' => 828 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.Oz0e6MmjbU/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] -fusion SPANNING read extraction for scaff: AC092807.3--DDAH1 [1000] [2000] [3000] [4000] [5000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHAC2 -fusion SPANNING read extraction for scaff: PMS2P12--AC004980.4 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1 [1000] [2000] [3000] [4000] [5000] [6000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 -filtered reads reasons: $VAR1 = { 'lacks exon overlap' => 752, 'seq similar region alignment' => 3815 }; EM: Starting log likelihood: -961.246992 EM: Round [1] log likelihood: -961.175633 EM: Round [2] log likelihood: -961.156723 EM: Round [3] log likelihood: -961.138975 EM: Round [4] log likelihood: -961.122287 EM: Round [5] log likelihood: -961.106582 EM: Round [6] log likelihood: -961.091787 EM: Round [7] log likelihood: -961.077837 EM: Round [8] log likelihood: -961.064673 EM: Round [9] log likelihood: -961.052241 EM: Round [10] log likelihood: -961.040493 EM: Round [11] log likelihood: -961.029384 EM: Round [12] log likelihood: -961.018872 EM: Round [13] log likelihood: -961.008919 EM: Round [14] log likelihood: -960.999491 EM: Round [15] log likelihood: -960.990555 EM: Round [16] log likelihood: -960.982081 EM: Round [17] log likelihood: -960.974043 EM: Round [18] log likelihood: -960.966414 EM: Round [19] log likelihood: -960.959171 EM: Round [20] log likelihood: -960.952292 EM: Round [21] log likelihood: -960.945755 EM: Round [22] log likelihood: -960.939543 EM: Round [23] log likelihood: -960.933637 EM: Round [24] log likelihood: -960.928019 EM: Round [25] log likelihood: -960.922676 EM: Round [26] log likelihood: -960.917591 EM: Round [27] log likelihood: -960.912752 EM: Round [28] log likelihood: -960.908144 EM: Round [29] log likelihood: -960.903757 EM: Round [30] log likelihood: -960.899579 EM: Round [31] log likelihood: -960.895598 EM: Round [32] log likelihood: -960.891805 EM: Round [33] log likelihood: -960.888191 EM: Round [34] log likelihood: -960.884746 EM: Round [35] log likelihood: -960.881462 EM: Round [36] log likelihood: -960.878330 EM: Round [37] log likelihood: -960.875344 EM: Round [38] log likelihood: -960.872496 EM: Round [39] log likelihood: -960.869780 EM: Round [40] log likelihood: -960.867188 EM: Round [41] log likelihood: -960.864716 EM: Round [42] log likelihood: -960.862356 EM: Round [43] log likelihood: -960.860105 EM: Round [44] log likelihood: -960.857956 EM: Round [45] log likelihood: -960.855905 EM: Round [46] log likelihood: -960.853946 EM: Round [47] log likelihood: -960.852077 EM: Round [48] log likelihood: -960.850292 EM: Round [49] log likelihood: -960.848588 EM: Round [50] log likelihood: -960.846960 EM: Round [51] log likelihood: -960.845406 EM: Round [52] log likelihood: -960.843922 EM: Round [53] log likelihood: -960.842504 EM: Round [54] log likelihood: -960.841150 EM: Round [55] log likelihood: -960.839856 EM: Round [56] log likelihood: -960.838620 EM: Round [57] log likelihood: -960.837439 EM: Round [58] log likelihood: -960.836311 EM: Round [59] log likelihood: -960.835234 EM: Round [60] log likelihood: -960.834204 EM: Round [61] log likelihood: -960.833220 EM: Round [62] log likelihood: -960.832279 EM: Round [63] log likelihood: -960.831380 EM: Round [64] log likelihood: -960.830521 EM: Round [65] log likelihood: -960.829701 EM: Round [66] log likelihood: -960.828916 EM: Round [67] log likelihood: -960.828166 EM: Round [68] log likelihood: -960.827450 EM: Round [69] log likelihood: -960.826764 EM: Round [70] log likelihood: -960.826110 EM: Round [71] log likelihood: -960.825484 EM: Round [72] log likelihood: -960.824885 EM: Round [73] log likelihood: -960.824313 EM: Round [74] log likelihood: -960.823766 EM: Round [75] log likelihood: -960.823244 EM: Round [76] log likelihood: -960.822744 EM: Round [77] log likelihood: -960.822266 EM: Round [78] log likelihood: -960.821809 EM: Round [79] log likelihood: -960.821372 EM: Round [80] log likelihood: -960.820955 EM: Round [81] log likelihood: -960.820555 EM: Round [82] log likelihood: -960.820173 EM: Round [83] log likelihood: -960.819808 EM: Round [84] log likelihood: -960.819459 EM: Round [85] log likelihood: -960.819125 EM: Round [86] log likelihood: -960.818806 EM: Round [87] log likelihood: -960.818501 EM: Round [88] log likelihood: -960.818209 EM: Round [89] log likelihood: -960.817930 EM: Round [90] log likelihood: -960.817663 EM: Round [91] log likelihood: -960.817408 EM: Round [92] log likelihood: -960.817164 EM: Round [93] log likelihood: -960.816931 EM: Round [94] log likelihood: -960.816708 EM: Round [95] log likelihood: -960.816494 EM: Round [96] log likelihood: -960.816290 EM: Round [97] log likelihood: -960.816095 EM: Round [98] log likelihood: -960.815908 EM: Round [99] log likelihood: -960.815730 EM: Round [100] log likelihood: -960.815559 EM: Round [101] log likelihood: -960.815396 EM: Round [102] log likelihood: -960.815240 EM: Round [103] log likelihood: -960.815090 EM: Round [104] log likelihood: -960.814947 EM: Round [105] log likelihood: -960.814811 EM: Round [106] log likelihood: -960.814680 EM: Round [107] log likelihood: -960.814555 EM: Round [108] log likelihood: -960.814436 EM: Round [109] log likelihood: -960.814321 EM: Round [110] log likelihood: -960.814212 EM: Round [111] log likelihood: -960.814108 EM: Round [112] log likelihood: -960.814008 EM: Stopping iterations at round 112 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.Oz0e6MmjbU/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.consolidated.bam into /tmp/nxf.Oz0e6MmjbU/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.Oz0e6MmjbU/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.Oz0e6MmjbU/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.consolidated.bam.read_coords > /tmp/nxf.Oz0e6MmjbU/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.Oz0e6MmjbU/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.consolidated.bam.frag_coords > /tmp/nxf.Oz0e6MmjbU/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.Oz0e6MmjbU/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.Oz0e6MmjbU/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.Oz0e6MmjbU/fi_workdir/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.Oz0e6MmjbU/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.Oz0e6MmjbU/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -907.467560 EM: Round [1] log likelihood: -907.416349 EM: Round [2] log likelihood: -907.416338 EM: Stopping iterations at round 2 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.Oz0e6MmjbU/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1_1.fastp.fastq.gz: 296375716 Warning - not locating file: /tmp/nxf.Oz0e6MmjbU/aih-tih-sc-96d0f6-R1_A23YTGFLT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000226049.3] WARNING, no entry stored in dbm for [ENSG00000260404.3] WARNING, no entry stored in dbm for [ENSG00000280136.2] WARNING, no entry stored in dbm for [ENSG00000228546.2] WARNING, no entry stored in dbm for [ENSG00000250778.1] WARNING, no entry stored in dbm for [ENSG00000228546.2] WARNING, no entry stored in dbm for [ENSG00000250778.1] ls: cannot access 'IGV_inputs': No such file or directory