[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3377ac-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	328561947
Known Splicing Events:	326362330
Partial Novel Splicing Events:	1336873
Novel Splicing Events:	766783
Filtered Splicing Events:	95961

Total splicing  Junctions:	451740
Known Splicing Junctions:	256672
Partial Novel Splicing Junctions:	115724
Novel Splicing Junctions:	79344

===================================================================
Create BED file ...
Create Interact file ...