Done. -parsing GTF file: /tmp/nxf.BeuoTtRGkb/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.BeuoTtRGkb/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.BeuoTtRGkb/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.BeuoTtRGkb/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.BeuoTtRGkb/fi_workdir/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.BeuoTtRGkb/fi_workdir/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam 11:14:04 : INFO : Done. -parsing GTF file: /tmp/nxf.BeuoTtRGkb/fi_workdir/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.gtf -parsing /tmp/nxf.BeuoTtRGkb/fi_workdir/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] [140000] [150000] [160000] [170000] [180000] [190000] [200000] [210000] [220000] [230000] [240000] [250000] [260000] [270000] [280000] [290000] [300000] [310000] [320000] [330000] [340000] [350000] [360000] [370000] [380000] [390000] [400000] [410000] [420000] [430000] -done parsing /tmp/nxf.BeuoTtRGkb/fi_workdir/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'exons hit < 2' => 4, 'small anchor length' => 37, 'excessive soft clipping' => 2239, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 52, 'per_id < 96' => 1893, 'seq-similar region overlap' => 108, ' ** passed ** ' => 6190, 'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 3 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.BeuoTtRGkb/fi_workdir/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] -fusion SPANNING read extraction for scaff: AC013268.1--MALL [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] -fusion SPANNING read extraction for scaff: AC092807.3--DDAH1 [1000] [2000] [3000] [4000] [5000] -fusion SPANNING read extraction for scaff: GTF2A1L--STON1-GTF2A1L [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGC3 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGC4 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGC5 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA10 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA8 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGB6 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA11 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGB7 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA12 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA9 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] -fusion SPANNING read extraction for scaff: PRSS27--TPSB2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] -fusion SPANNING read extraction for scaff: STON1--GTF2A1L [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1 [1000] [2000] [3000] [4000] [5000] [6000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 -filtered reads reasons: $VAR1 = { 'seq similar region alignment' => 34175, 'lacks exon overlap' => 1353 }; EM: Starting log likelihood: -2791.944583 EM: Round [1] log likelihood: -2791.538389 EM: Round [2] log likelihood: -2791.217715 EM: Round [3] log likelihood: -2790.955041 EM: Round [4] log likelihood: -2790.738224 EM: Round [5] log likelihood: -2790.558200 EM: Round [6] log likelihood: -2790.408013 EM: Round [7] log likelihood: -2790.282231 EM: Round [8] log likelihood: -2790.176554 EM: Round [9] log likelihood: -2790.087534 EM: Round [10] log likelihood: -2790.012383 EM: Round [11] log likelihood: -2789.948824 EM: Round [12] log likelihood: -2789.894987 EM: Round [13] log likelihood: -2789.849327 EM: Round [14] log likelihood: -2789.810561 EM: Round [15] log likelihood: -2789.777617 EM: Round [16] log likelihood: -2789.749599 EM: Round [17] log likelihood: -2789.725756 EM: Round [18] log likelihood: -2789.705454 EM: Round [19] log likelihood: -2789.688160 EM: Round [20] log likelihood: -2789.673421 EM: Round [21] log likelihood: -2789.660856 EM: Round [22] log likelihood: -2789.650142 EM: Round [23] log likelihood: -2789.641002 EM: Round [24] log likelihood: -2789.633206 EM: Round [25] log likelihood: -2789.626553 EM: Round [26] log likelihood: -2789.620875 EM: Round [27] log likelihood: -2789.616029 EM: Round [28] log likelihood: -2789.611892 EM: Round [29] log likelihood: -2789.608361 EM: Round [30] log likelihood: -2789.605345 EM: Round [31] log likelihood: -2789.602771 EM: Round [32] log likelihood: -2789.600573 EM: Round [33] log likelihood: -2789.598696 EM: Round [34] log likelihood: -2789.597093 EM: Round [35] log likelihood: -2789.595724 EM: Round [36] log likelihood: -2789.594555 EM: Round [37] log likelihood: -2789.593557 EM: Round [38] log likelihood: -2789.592704 EM: Round [39] log likelihood: -2789.591976 EM: Round [40] log likelihood: -2789.591354 EM: Round [41] log likelihood: -2789.590822 EM: Round [42] log likelihood: -2789.590369 EM: Round [43] log likelihood: -2789.589981 EM: Round [44] log likelihood: -2789.589650 EM: Round [45] log likelihood: -2789.589367 EM: Round [46] log likelihood: -2789.589126 EM: Round [47] log likelihood: -2789.588920 EM: Round [48] log likelihood: -2789.588744 EM: Round [49] log likelihood: -2789.588593 EM: Round [50] log likelihood: -2789.588465 EM: Round [51] log likelihood: -2789.588355 EM: Round [52] log likelihood: -2789.588261 EM: Stopping iterations at round 52 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.BeuoTtRGkb/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.consolidated.bam into /tmp/nxf.BeuoTtRGkb/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.BeuoTtRGkb/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.BeuoTtRGkb/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.consolidated.bam.read_coords > /tmp/nxf.BeuoTtRGkb/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.BeuoTtRGkb/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.consolidated.bam.frag_coords > /tmp/nxf.BeuoTtRGkb/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.BeuoTtRGkb/fi_workdir/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.BeuoTtRGkb/fi_workdir/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.BeuoTtRGkb/fi_workdir/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.BeuoTtRGkb/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.BeuoTtRGkb/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -2576.127962 EM: Round [1] log likelihood: -2576.117774 EM: Round [2] log likelihood: -2576.117694 EM: Stopping iterations at round 2 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.BeuoTtRGkb/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1_1.fastp.fastq.gz: 297220257 Warning - not locating file: /tmp/nxf.BeuoTtRGkb/aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000280136.2] WARNING, no entry stored in dbm for [ENSG00000186148.13] WARNING, no entry stored in dbm for [ENSG00000186148.13] WARNING, no entry stored in dbm for [ENSG00000186148.13] WARNING, no entry stored in dbm for [ENSG00000226049.3] WARNING, no entry stored in dbm for [ENSG00000260404.3] WARNING, no entry stored in dbm for [ENSG00000068781.21] WARNING, no entry stored in dbm for [ENSG00000068781.21] ls: cannot access 'IGV_inputs': No such file or directory