[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01c779-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	262848966
Known Splicing Events:	260209210
Partial Novel Splicing Events:	1489642
Novel Splicing Events:	1038072
Filtered Splicing Events:	112042

Total splicing  Junctions:	538363
Known Splicing Junctions:	263232
Partial Novel Splicing Junctions:	143289
Novel Splicing Junctions:	131842

===================================================================
Create BED file ...
Create Interact file ...