File Info

Filename
.command.log
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d2/b7047a02afd31be07e7f36355237b0/.command.log
Size
70.3 KB
Task
NFCORE_RNAFUSION:RNAFUSION:FUSIONINSPECTOR_WORKFLOW:FUSIONINSPECTOR (aih-tih-sc-9e2647-R1_A23YTGFLT4_1)
Attempt

Content

View in new tab 
  Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/c4/e6f033bbc6e50511d9c3f5a4b42e06/aih-tih-sc-9e2647-R1_A23YTGFLT4_1_2.fastp.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d2/b7047a02afd31be07e7f36355237b0/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/80/73452805e862ae4e94fe4a771be015/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusionreport.tsv
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d2/b7047a02afd31be07e7f36355237b0/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/c4/e6f033bbc6e50511d9c3f5a4b42e06/aih-tih-sc-9e2647-R1_A23YTGFLT4_1_1.fastp.fastq.gz
==> STAGING COMPLETE (6 inputs)

Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_pair_to_mini_genome_join.pl --fusions aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusionreport.tsv --gtf /tmp/nxf.tjUv3WLwez/ctat_genome_lib_build_dir/ref_annot.gtf --genome_fa /tmp/nxf.tjUv3WLwez/ctat_genome_lib_build_dir/ref_genome.fa --out_prefix /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1 --shrink_introns --max_intron_length 1000 
Done.
Execution Time = 0.12 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_pair_to_mini_genome_join.pl --fusions aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusionreport.tsv --gtf /tmp/nxf.tjUv3WLwez/ctat_genome_lib_build_dir/ref_annot.gtf --genome_fa /tmp/nxf.tjUv3WLwez/ctat_genome_lib_build_dir/ref_genome.fa --out_prefix /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1 --shrink_introns --max_intron_length 1000 
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/fusion_contigs.ok
Running: cp /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa
Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/cp_contigs_file_workdir
Running: cp /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf
Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/cp_gtf_file_workdir.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fasta_and_gtf_to_cytoband.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf > /tmp/nxf.tjUv3WLwez/cytoBand.txt
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fasta_and_gtf_to_cytoband.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf > /tmp/nxf.tjUv3WLwez/cytoBand.txt
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/cytoband.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/gtf_gene_to_bed.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.bed
-parsing GTF file: /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/gtf_gene_to_bed.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.bed
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/merged_contig_gtf_to_bed.ok
Running: sort -k1,1 -k2,2n /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.bed > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: sort -k1,1 -k2,2n /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.bed > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.bed.sorted.bed
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.bed.bedsort.ok
Running: bgzip -f /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: bgzip -f /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.bed.sorted.bed
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.bed.bgzip.ok
Running: tabix -p bed /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.bed.sorted.bed.gz
Execution Time = 0.00 minutes. CMD: tabix -p bed /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.bed.sorted.bed.gz
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.bed.tabix.ok
Running: samtools faidx /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa
Execution Time = 0.00 minutes. CMD: samtools faidx /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/merged_contig_fai.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/run_FI_STAR.pl --genome /tmp/nxf.tjUv3WLwez/ctat_genome_lib_build_dir/ref_genome.fa --patch /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa --max_mate_dist 100000 -G /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf --CPU 8 --out_prefix aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star --out_dir /tmp/nxf.tjUv3WLwez/fi_workdir --reads "/tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1_2.fastp.fastq.gz"
* Running CMD: /usr/local/bin/STAR  --runThreadN 8  --genomeDir /tmp/nxf.tjUv3WLwez/ctat_genome_lib_build_dir/ref_genome.fa.star.idx  --outSAMtype BAM SortedByCoordinate  --twopassMode None  --alignSJDBoverhangMin 10  --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705  --alignInsertionFlush Right   --alignMatesGapMax 100000  --alignIntronMax 100000  --outSAMattributes All  --readFilesIn /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1_2.fastp.fastq.gz  --genomeFastaFiles /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa  --outSAMfilter KeepAllAddedReferences  --sjdbGTFfile /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf  --alignSJstitchMismatchNmax 5 -1 5 5  --scoreGapNoncan -6  --readFilesCommand 'gunzip -c' 
Jun 08 10:35:21 ..... started STAR run
Jun 08 10:35:21 ..... loading genome
Jun 08 10:37:01 ... generating Suffix Array index
Jun 08 10:40:46 ... completed Suffix Array index
Jun 08 10:40:46 ..... processing annotations GTF
Jun 08 10:40:47 ..... inserting junctions into the genome indices
Jun 08 10:41:48 ..... started mapping
Jun 08 11:38:25 ..... finished mapping
Jun 08 11:38:27 ..... started sorting BAM
Jun 08 11:38:28 ..... finished successfully
* Running CMD: mv Aligned.sortedByCoord.out.bam aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam
* Running CMD: samtools index aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam
Execution Time = 63.14 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/run_FI_STAR.pl --genome /tmp/nxf.tjUv3WLwez/ctat_genome_lib_build_dir/ref_genome.fa --patch /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa --max_mate_dist 100000 -G /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf --CPU 8 --out_prefix aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star --out_dir /tmp/nxf.tjUv3WLwez/fi_workdir --reads "/tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1_2.fastp.fastq.gz"
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/run_STAR.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/bam_mark_duplicates.py  -i /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam   -o /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam   --remove_dups 
11:38:41 : INFO : Done.
Execution Time = 0.19 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/bam_mark_duplicates.py  -i /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam   -o /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam   --remove_dups 
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/mark_dup_reads.ok
Running: samtools index /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam
Execution Time = 0.02 minutes. CMD: samtools index /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/mark_dups_reads.index.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_JUNCTION_reads_from_fusion_contig_bam.pl --gtf_file /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam
-parsing GTF file: /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf
-parsing /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam

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[2110000]   -done parsing /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.  Extracting junction info.
junction read elimination tally: $VAR1 = {
          'low complexity anchor region' => 1,
          'per_id < 96' => 7372,
          'excessive soft clipping' => 14565,
          'small anchor length' => 1,
          'num genes matched < 2' => 59,
          'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 4,
          ' ** passed ** ' => 6797,
          'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 277,
          'num_hits: 4 != num_counted_on_fusion_contigs 2 ' => 12,
          'num_hits: 3 != num_counted_on_fusion_contigs 2 ' => 6
        };
-writing fusion junction support info.
Execution Time = 1.15 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_JUNCTION_reads_from_fusion_contig_bam.pl --gtf_file /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_SPANNING_reads_from_bam.from_chim_summary.pl --gtf_file /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam --junction_info /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_junction_info --genome_lib_dir ctat_genome_lib_build_dir   > /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_span_reads.sam
-outputting the spanning read info: /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info.
 - counting read alignments among fusion contigs.

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[12000]   -fusion SPANNING read extraction for scaff: AC020550.2--APBA1

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[88000]   -fusion SPANNING read extraction for scaff: AC073264.3--TCAF1

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[26000]   -fusion SPANNING read extraction for scaff: AC079781.4--ASNS

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[3000]   -fusion SPANNING read extraction for scaff: AC092807.3--DDAH1

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[12000]   -fusion SPANNING read extraction for scaff: AC104041.1--APBA1

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[15000]   -fusion SPANNING read extraction for scaff: AL137803.1--SLC44A5

[1000]   -fusion SPANNING read extraction for scaff: CCDC169--SOHLH2

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[161000]   
[162000]   -fusion SPANNING read extraction for scaff: CREBBP--ZSCAN32

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[29000]   -fusion SPANNING read extraction for scaff: CTIF--SLC14A2

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[23000]   
[24000]   -fusion SPANNING read extraction for scaff: DDX10--LINC02550

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[25000]   
[26000]   -fusion SPANNING read extraction for scaff: DDX10--C11orf53

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[176000]   
[177000]   -fusion SPANNING read extraction for scaff: FAM193A--HTT

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[31000]   
[32000]   -fusion SPANNING read extraction for scaff: GLTP--USP30

[1000]   
[2000]   -fusion SPANNING read extraction for scaff: GUCY1B2--NKAIN2

[1000]   
[2000]   -fusion SPANNING read extraction for scaff: LINC00381--NKAIN2

[1000]   
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[294000]   -fusion SPANNING read extraction for scaff: LINC02888--AHR

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[219000]   -fusion SPANNING read extraction for scaff: RAB3IP--LYZ

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[38000]   
[39000]   -fusion SPANNING read extraction for scaff: SCAPER--NEO1

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[90000]   -fusion SPANNING read extraction for scaff: STX16--NPEPL1

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[32000]   -fusion SPANNING read extraction for scaff: TLK2--AC240565.1

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[5000]   -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1

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[59000]   -fusion SPANNING read extraction for scaff: TRAPPC12--NPM1P48

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[59000]   -fusion SPANNING read extraction for scaff: TRAPPC12--LINC01249

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[37000]   -fusion SPANNING read extraction for scaff: TVP23C--CDRT4

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[17000]   -fusion SPANNING read extraction for scaff: USP30--GRIP1

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[637000]   -fusion SPANNING read extraction for scaff: ZFC3H1--ZNF385D
-filtered reads reasons: $VAR1 = {
          'seq similar region alignment' => 12816,
          'lacks exon overlap' => 16851
        };
Execution Time = 3.16 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_SPANNING_reads_from_bam.from_chim_summary.pl --gtf_file /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam --junction_info /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_junction_info --genome_lib_dir ctat_genome_lib_build_dir   > /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_span_reads.sam
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/coalesce_junction_and_spanning_info.pl /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_junction_info /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info 1  > /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/coalesce_junction_and_spanning_info.pl /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_junction_info /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info 1  > /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/coalesce_junc_n_span.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary > /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj
EM: Starting log likelihood: -10290.049322
EM: Round [1] log likelihood: -10260.996256
EM: Round [2] log likelihood: -10255.028710
EM: Round [3] log likelihood: -10252.991376
EM: Round [4] log likelihood: -10252.075598
EM: Round [5] log likelihood: -10251.500847
EM: Round [6] log likelihood: -10251.080797
EM: Round [7] log likelihood: -10250.761797
EM: Round [8] log likelihood: -10250.516976
EM: Round [9] log likelihood: -10250.328145
EM: Round [10] log likelihood: -10250.182007
EM: Round [11] log likelihood: -10250.068623
EM: Round [12] log likelihood: -10249.980481
EM: Round [13] log likelihood: -10249.911861
EM: Round [14] log likelihood: -10249.858376
EM: Round [15] log likelihood: -10249.816650
EM: Round [16] log likelihood: -10249.784077
EM: Round [17] log likelihood: -10249.758633
EM: Round [18] log likelihood: -10249.738751
EM: Round [19] log likelihood: -10249.723210
EM: Round [20] log likelihood: -10249.711058
EM: Round [21] log likelihood: -10249.701555
EM: Round [22] log likelihood: -10249.694122
EM: Round [23] log likelihood: -10249.688307
EM: Round [24] log likelihood: -10249.683758
EM: Round [25] log likelihood: -10249.680199
EM: Round [26] log likelihood: -10249.677414
EM: Round [27] log likelihood: -10249.675235
EM: Round [28] log likelihood: -10249.673530
EM: Round [29] log likelihood: -10249.672196
EM: Round [30] log likelihood: -10249.671152
EM: Round [31] log likelihood: -10249.670334
EM: Round [32] log likelihood: -10249.669695
EM: Round [33] log likelihood: -10249.669195
EM: Round [34] log likelihood: -10249.668803
EM: Round [35] log likelihood: -10249.668497
EM: Round [36] log likelihood: -10249.668257
EM: Round [37] log likelihood: -10249.668069
EM: Round [38] log likelihood: -10249.667922
EM: Round [39] log likelihood: -10249.667807
EM: Round [40] log likelihood: -10249.667717
EM: Stopping iterations at round 40 due to insufficient improvement in likelihood.
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary > /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/init_EM_adj_counts.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/filter_fusions_by_frag_thresholds.pl --min_junction_reads 0 --min_sum_frags 1 --min_novel_junction_support 3 --min_spanning_frags_only 5 --fusion_preds /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj --require_LDAS 1 > /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/filter_fusions_by_frag_thresholds.pl --min_junction_reads 0 --min_sum_frags 1 --min_novel_junction_support 3 --min_spanning_frags_only 5 --fusion_preds /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj --require_LDAS 1 > /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/filter_by_frag_threshs.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary LeftGene,RightGene,JunctionReads  > /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.fusion_junction_read_accs
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary LeftGene,RightGene,JunctionReads  > /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.fusion_junction_read_accs
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/prep_igv_extract_junc_reads.ok
Running: bash -c "set -eof pipefail; /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_junction_reads_by_accession.pl /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.fusion_junction_read_accs /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam | samtools view -@ 8 -bT /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa -  | samtools sort -@ 8 - -o /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam"
[bam_sort_core] merging from 0 files and 8 in-memory blocks...
Execution Time = 0.00 minutes. CMD: bash -c "set -eof pipefail; /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_junction_reads_by_accession.pl /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.fusion_junction_read_accs /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam | samtools view -@ 8 -bT /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa -  | samtools sort -@ 8 - -o /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam"
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/prep_igv_junc_reads_bam.ok
Running: samtools index /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam
Execution Time = 0.00 minutes. CMD: samtools index /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/samtools_idx_junc_reads_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam.bed
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam.bed
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam.bam_to_bed.ok
Running: sort -k1,1 -k2,2n /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam.bed > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: sort -k1,1 -k2,2n /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam.bed > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam.bed.sorted.bed
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam.bedsort.ok
Running: bgzip -f /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: bgzip -f /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam.bed.sorted.bed
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam.bgzip.ok
Running: tabix -p bed /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam.bed.sorted.bed.gz
Execution Time = 0.00 minutes. CMD: tabix -p bed /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam.bed.sorted.bed.gz
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.junction_reads.bam.tabix.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary LeftGene,RightGene,SpanningFrags  > /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.fusion_spanning_read_accs
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary LeftGene,RightGene,SpanningFrags  > /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.fusion_spanning_read_accs
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/span_reads_acc.ok
Running: samtools view -H /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam -b -o /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam
Execution Time = 0.00 minutes. CMD: samtools view -H /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam -b -o /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/init_spanning_reads_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_spanning_reads_by_accession.pl /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.fusion_spanning_read_accs /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_span_reads.sam | samtools view -@ 8 -bT /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa -  | samtools sort -@ 8 - -o /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam || : 
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_spanning_reads_by_accession.pl /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.fusion_spanning_read_accs /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_span_reads.sam | samtools view -@ 8 -bT /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa -  | samtools sort -@ 8 - -o /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam || : 
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/prep_spanning_reads.ok
Running: samtools index /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam
Execution Time = 0.00 minutes. CMD: samtools index /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/samtools_index_span_reads_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam.bed
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam.bed
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam.bam_to_bed.ok
Running: sort -k1,1 -k2,2n /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam.bed > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: sort -k1,1 -k2,2n /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam.bed > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam.bed.sorted.bed
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam.bedsort.ok
Running: bgzip -f /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: bgzip -f /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam.bed.sorted.bed
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam.bgzip.ok
Running: tabix -p bed /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam.bed.sorted.bed.gz
Execution Time = 0.00 minutes. CMD: tabix -p bed /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam.bed.sorted.bed.gz
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.spanning_reads.bam.tabix.ok
Running: cp /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.consolidated.bam
Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.consolidated.bam
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/cp_consol_bam.ok
Running: samtools index /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.consolidated.bam
Execution Time = 0.02 minutes. CMD: samtools index /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.consolidated.bam
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/index_consol_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_to_frag_coords.pl --sam /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.consolidated.bam --max_insert_size 10000000 
-extracting read coordinates from /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.consolidated.bam into /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.consolidated.bam.read_coords

CMD: touch /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.consolidated.bam.read_coords.ok
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.consolidated.bam.read_coords > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.consolidated.bam.read_coords.sort_by_readname
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.consolidated.bam.frag_coords > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.consolidated.bam.frag_coords.coord_sorted
Execution Time = 0.54 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_to_frag_coords.pl --sam /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.consolidated.bam --max_insert_size 10000000 
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/prep_igv_sam_frag_coordss.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_summary_to_igv_JuncSpan_fmt.pl /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.consolidated.bam.frag_coords > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.igv.FusionJuncSpan
Execution Time = 0.01 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_summary_to_igv_JuncSpan_fmt.pl /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.consolidated.bam.frag_coords > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.igv.FusionJuncSpan
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/prep_igv_juncspan_fmt.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_pfam_domain_info.pl --finspector_gtf /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.igv.Pfam.gff3 
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_pfam_domain_info.pl --finspector_gtf /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.igv.Pfam.gff3 
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/prep_igv_pfam_gff3.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.igv.Pfam.gff3 > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.igv.Pfam.bed
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.igv.Pfam.gff3 > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.igv.Pfam.bed
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/prep_igv_pfam_bed.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_seq_similar_region_FI_coordinates.pl --finspector_gtf /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.igv.seqsimilar.gff3 
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_seq_similar_region_FI_coordinates.pl --finspector_gtf /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.igv.seqsimilar.gff3 
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/prep_igv_seqsim_gff3.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.igv.seqsimilar.gff3 > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.igv.seqsimilar.bed
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.igv.seqsimilar.gff3 > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.igv.seqsimilar.bed
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/prep_igv_seqsim_bed.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/append_breakpoint_junction_info_via_FI_contigs.pl /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa > /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/append_breakpoint_junction_info_via_FI_contigs.pl /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa > /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/add_splice_info.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/blast_and_promiscuity_filter.pl  --fusion_preds /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --out_prefix /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1 --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter  --genome_lib_dir ctat_genome_lib_build_dir  --max_promiscuity 10  --min_pct_dom_promiscuity 50 
CMD: cp /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/blast_and_promiscuity_filter.pl  --fusion_preds /tmp/nxf.tjUv3WLwez/fi_workdir/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --out_prefix /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1 --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/blast_filter.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/util/filter_by_annotation_rules.pl  --fusions /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter --genome_lib_dir /tmp/nxf.tjUv3WLwez/ctat_genome_lib_build_dir
-done, see /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/util/filter_by_annotation_rules.pl  --fusions /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter --genome_lib_dir /tmp/nxf.tjUv3WLwez/ctat_genome_lib_build_dir
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/annot_filter.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj
EM: Starting log likelihood: -10067.273138
EM: Round [1] log likelihood: -10046.090714
EM: Round [2] log likelihood: -10041.507176
EM: Round [3] log likelihood: -10040.464017
EM: Round [4] log likelihood: -10040.282780
EM: Round [5] log likelihood: -10040.258802
EM: Round [6] log likelihood: -10040.256113
EM: Round [7] log likelihood: -10040.255832
EM: Round [8] log likelihood: -10040.255803
EM: Stopping iterations at round 8 due to insufficient improvement in likelihood.
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/EM_adj_counts.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/incorporate_FFPM_into_final_report.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM
-total frags in /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1_1.fastp.fastq.gz: 296747855
Execution Time = 5.92 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/incorporate_FFPM_into_final_report.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/add_FFPM.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/find_microhomologies_by_kmer_matches.pl --fasta /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa --gtf /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf  > /tmp/nxf.tjUv3WLwez/fi_workdir/microH.dat
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/find_microhomologies_by_kmer_matches.pl --fasta /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fa --gtf /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gtf  > /tmp/nxf.tjUv3WLwez/fi_workdir/microH.dat
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/microH.dat.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/append_microH_distance.py /tmp/nxf.tjUv3WLwez/fi_workdir/microH.dat /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM  >  /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/append_microH_distance.py /tmp/nxf.tjUv3WLwez/fi_workdir/microH.dat /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM  >  /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/append_microH_info.ok
Running: cp /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.FusionInspector.fusions.tsv
Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.FusionInspector.fusions.tsv
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/cp_final.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/column_exclusions.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.FusionInspector.fusions.tsv JunctionReads,SpanningFrags,CounterFusionLeftReads,CounterFusionRightReads  > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/column_exclusions.pl /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.FusionInspector.fusions.tsv JunctionReads,SpanningFrags,CounterFusionLeftReads,CounterFusionRightReads  > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/final.abridged.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/create_fusion_inspector_igvjs.py --fusion_inspector_directory /tmp/nxf.tjUv3WLwez --json_outfile /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_inspector_web.json --file_prefix aih-tih-sc-9e2647-R1_A23YTGFLT4_1
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/create_fusion_inspector_igvjs.py --fusion_inspector_directory /tmp/nxf.tjUv3WLwez --json_outfile /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_inspector_web.json --file_prefix aih-tih-sc-9e2647-R1_A23YTGFLT4_1
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/create_fi_igvjs.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/fusion-reports/create_fusion_report.py --html_template /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_report_html_template/igvjs_fusion.html --fusions_json /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_inspector_web.json --input_file_prefix aih-tih-sc-9e2647-R1_A23YTGFLT4_1 --html_output /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_inspector_web.html
Warning - not locating file: /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.gmap_trinity_GG.fusions.gff3.bed
Execution Time = 0.01 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/fusion-reports/create_fusion_report.py --html_template /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_report_html_template/igvjs_fusion.html --fusions_json /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_inspector_web.json --input_file_prefix aih-tih-sc-9e2647-R1_A23YTGFLT4_1 --html_output /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.fusion_inspector_web.html
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/fusion_reports_html.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/FusionAnnotator --annotate /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv  --genome_lib_dir /tmp/nxf.tjUv3WLwez/ctat_genome_lib_build_dir > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv.annotated 
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/FusionAnnotator --annotate /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv  --genome_lib_dir /tmp/nxf.tjUv3WLwez/ctat_genome_lib_build_dir > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv.annotated 
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/fusion_annotator.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/util/fusion_to_coding_region_effect.pl --fusions /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv.annotated  --genome_lib_dir /tmp/nxf.tjUv3WLwez/ctat_genome_lib_build_dir > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv.annotated.coding_effect 
WARNING, no entry stored in dbm for [ENSG00000226240.2]
WARNING, no entry stored in dbm for [ENSG00000284627.1]
WARNING, no entry stored in dbm for [ENSG00000284627.1]
WARNING, no entry stored in dbm for [ENSG00000284627.1]
WARNING, no entry stored in dbm for [ENSG00000284627.1]
WARNING, no entry stored in dbm for [ENSG00000284627.1]
WARNING, no entry stored in dbm for [ENSG00000280136.2]
WARNING, no entry stored in dbm for [ENSG00000226049.3]
WARNING, no entry stored in dbm for [ENSG00000260404.3]
WARNING, no entry stored in dbm for [ENSG00000123201.14]
WARNING, no entry stored in dbm for [ENSG00000123201.14]
WARNING, no entry stored in dbm for [ENSG00000259692.6]
WARNING, no entry stored in dbm for [ENSG00000282057.1]
WARNING, no entry stored in dbm for [ENSG00000282057.1]
WARNING, no entry stored in dbm for [ENSG00000282057.1]
WARNING, no entry stored in dbm for [ENSG00000283537.2]
WARNING, no entry stored in dbm for [ENSG00000283537.2]
WARNING, no entry stored in dbm for [ENSG00000283537.2]
WARNING, no entry stored in dbm for [ENSG00000236318.2]
WARNING, no entry stored in dbm for [ENSG00000236318.2]
WARNING, no entry stored in dbm for [ENSG00000236318.2]
WARNING, no entry stored in dbm for [ENSG00000236318.2]
WARNING, no entry stored in dbm for [ENSG00000236318.2]
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/util/fusion_to_coding_region_effect.pl --fusions /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv.annotated  --genome_lib_dir /tmp/nxf.tjUv3WLwez/ctat_genome_lib_build_dir > /tmp/nxf.tjUv3WLwez/aih-tih-sc-9e2647-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv.annotated.coding_effect 
Running: touch /tmp/nxf.tjUv3WLwez/chckpts_dir/fusion_coding_region_effect.ok
ls: cannot access 'IGV_inputs': No such file or directory