FastQCFastQC Report
Wed 3 Jun 2026
NTC_0001_0001_A23YTGFLT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_A23YTGFLT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3897
Total Bases391.9 kbp
Sequences flagged as poor quality0
Sequence length16-151
%GC72

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90023.094688221709006No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC64216.474210931485757No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA3859.879394405953297No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1423.6438285860918658No Hit
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC681.7449319989735694No Hit
GACTGGAGTTCAGACGTGTGCTCTCCGATC220.5645368231973312Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC210.5388760585065435No Hit
GACTGGAGTTCAGACGTGTGCTCTTCCGATC200.5132152938157557Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC190.4875545291249679No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGC130.33358994098024125No Hit
TCTCTCTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC120.30792917628945343No Hit
TCTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC110.2822684115986656No Hit
TCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC80.2052861175263023No Hit
TCTCGATCCACGTCCCGGGAACGGTGCCGGGACTCCCTGTGTCTGTGGTC80.2052861175263023No Hit
GTGCCAATGGTGCTCCTGGTATTGCTGGTGCTCCTGGCTTCCCTGGTGCC70.1796253528355145No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGCG70.1796253528355145No Hit
TCTAGATGAGCAGCTGTCCCAGGTCCGAGAGGCCGACCGGCACCCGGGCA60.15396458814472672No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGC60.15396458814472672No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCC60.15396458814472672No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCG60.15396458814472672No Hit
ACCGTGCCCTCTCTCTCTCTCTCTCCCCC60.15396458814472672No Hit
CGCGCCCTCTCTCTCTCTCTCTCCCCC60.15396458814472672No Hit
TTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC60.15396458814472672No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT50.12830382345393893No Hit
TCTCTCTCTCTCTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC50.12830382345393893No Hit
GCGGGGGAGAGAGAGAGAGAGGGCGC50.12830382345393893No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCC50.12830382345393893No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGC50.12830382345393893No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC50.12830382345393893No Hit
TCTCTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC50.12830382345393893No Hit
GCGGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA50.12830382345393893No Hit
CTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT50.12830382345393893No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCC40.10264305876315115No Hit
CTTCGCGCCCTCTCTCTCTCTC40.10264305876315115No Hit
TCTCTCTCTCTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC40.10264305876315115No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCCG40.10264305876315115No Hit
GGGGGAGAGAGAGAGAGAGAGGGC40.10264305876315115No Hit
CTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT40.10264305876315115No Hit
GACCGAACGGGTGTCTGTTGAAAAAGACTCCGATGACTTGTGGTTAGGGG40.10264305876315115No Hit
CTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT40.10264305876315115No Hit
CTTCGCGCCCTCTCTCTCTCTCTCCCCC40.10264305876315115No Hit
GCTGCCCGAGTGTTACAGCCCCCCCGGCAGC40.10264305876315115No Hit
CTCTCTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT40.10264305876315115No Hit
GCTGCTGCCGGGGGGGC40.10264305876315115No Hit
CCCCGTCCTCCCCCCTCCCCGGGG40.10264305876315115No Hit
TCTCTCTCTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC40.10264305876315115No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCC40.10264305876315115No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)