FastQCFastQC Report
Wed 3 Jun 2026
NTC_0001_0001_A23YTGFLT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_A23YTGFLT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3897
Total Bases388.1 kbp
Sequences flagged as poor quality0
Sequence length16-151
%GC64

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA47712.240184757505773No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG2957.569925583782397No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC2847.2876571721837315No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT2295.876315114190403No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACCAAGACTCAAATCTGGGG1002.5660764690787787Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGGGG671.7192712342827816TruSeq Adapter, Index 7 (96% over 30bp)
GCTGCCGGGGGGGCTGTAACACTCGGCAGC661.6936104695919936No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACCAAGACTCAAATCTGGGG421.077752117013087Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGG391.0007698229407236TruSeq Adapter, Index 7 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCGTCACCAAGACTCAAATCTGGGG380.9751090582499358Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCACACGTCTGAACCCAGTCACCAAGACTCAAATCTGGGG350.8981267641775725Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAGAGCACACGTCTAACTCCAGTCACCAAGACTCAAATCTGGGG280.718501411342058Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGG280.718501411342058TruSeq Adapter, Index 2 (96% over 32bp)
GATCGGAGAGCACACGTCTGAACTCCAGTC230.590197587888119Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAGAGCACACGTCGAACTCCAGTCACCAAGACTCAAATCTGGGG220.5645368231973312No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAG220.5645368231973312No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTC210.5388760585065435Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCCACGTCTGAACTCCAGTCACCAAGACTCAAATCTGGGG210.5388760585065435TruSeq Adapter, Index 2 (95% over 23bp)
GATCGGAAGAGCACACGTTGAACTCCAGTCACCAAGACTCAAATCTGGGG200.5132152938157557No Hit
GATCGGAAGAGCACAGTCTGAACTCCAGTCACCAAGACTCAAATCTGGGG200.5132152938157557No Hit
GATCGGAAGAGCACACTCTGAACTCCAGTCACCAAGACTCAAATCTGGGG190.4875545291249679No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGC190.4875545291249679No Hit
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACCAAGACTCAAATCTGG180.4618937644341801Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCAACGTCTGAACTCCAGTCACCAAGACTCAAATCTGGGG160.4105722350526046TruSeq Adapter, Index 7 (95% over 22bp)
GATCGGAAGAGCACCGTCTGAACTCCAGTCACCAAGACTCAAATCTGGGG160.4105722350526046TruSeq Adapter, Index 2 (95% over 21bp)
GATCGGAAGAGCACACGTCTGAATCCAGTCACCAAGACTCAAATCTGGGG150.3849114703618168Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACCTCCAGTCACCAAGACTCAAATCTGG130.33358994098024125Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GCGCCCTCTCTCTCTCTCTCTCCCCCGC130.33358994098024125No Hit
GATCGGAAGAGCACACGCTGAACTCCAGTCACCAAGACTCAAATCTGGGG120.30792917628945343No Hit
GATCGGAAGAGCACACGGTCTGAACTCCAGTCACCAAGACTCAAATCTGG110.2822684115986656No Hit
GATCGGAAGAGCACACCGTCTGAACTCCAGTCACCAAGACTCAAATCTGG110.2822684115986656TruSeq Adapter, Index 7 (95% over 21bp)
GATCGGAAGAGCACACGTCCTGAACTCCAGTCACCAAGACTCAAATCTGG100.25660764690787785No Hit
GATCGGAAGAGCACACGTCTTGAACTCCAGTCACCAAGACTCAAATCTGG100.25660764690787785No Hit
GATCGGAAGAGCACACGTTCTGAACTCCAGTCACCAAGACTCAAATCTGG90.23094688221709006No Hit
GATCGGAAGAGCAACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGG90.23094688221709006TruSeq Adapter, Index 2 (95% over 24bp)
GATCGGAAGAGCACAACGTCTGAACTCCAGTCACCAAGACTCAAATCTGG90.23094688221709006TruSeq Adapter, Index 2 (95% over 22bp)
GGACCACTATGCCTCACACCGTGACCACAACCACAGAGACGAGACCCACG80.2052861175263023No Hit
GATCGGAAGAGCACCACGTCTGAACTCCAGTCACCAAGACTCAAATCTGG80.2052861175263023TruSeq Adapter, Index 7 (95% over 23bp)
GATCGGAAGAGCACACGTCTGAAACTCCAGTCACCAAGACTCAAATCTGG80.2052861175263023Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GATCGGAAGAGCCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGG80.2052861175263023TruSeq Adapter, Index 7 (96% over 25bp)
GGCTCAGTTTCCCTGTCTTTAAAATGGGCATAACAATTCCTGGGTCCTGG70.1796253528355145No Hit
CACCGCTGTTACCCTTGGGACCAGGAGGGCCGCCGGGGCCCTGGGGTCCA70.1796253528355145No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACCAAGACTCAAATCTAGGG70.1796253528355145Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CGCGCCCTCTCTCTCTCTCTCTCCCCCGC70.1796253528355145No Hit
GATCGGAAGAGCACACGTCTAACTCCAGTCACCAAGACTCAAATCTAGGG70.1796253528355145Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGGGGAGAGAGAGAGAGAGAGGGCGCG60.15396458814472672No Hit
GATCGGAAGAGCCACGTCTGAACTCCAGTCACCAAGACTCAAATCTAGGG60.15396458814472672TruSeq Adapter, Index 2 (95% over 23bp)
GGGGGAGAGAGAGAGAGAGAGGGCACGGT60.15396458814472672No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGC60.15396458814472672No Hit
CTGCACCAGCTGCATGAGGAAGTTCCCGTCTGGGGCTGCCCGGGTGCCGG60.15396458814472672No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCC60.15396458814472672No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTAG60.15396458814472672TruSeq Adapter, Index 7 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTTCCAGTCACCAAGACTCAAATCTGG60.15396458814472672Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CGCCCTCTCTCTCTCTCTCTCCCCCGC60.15396458814472672No Hit
GCGCCCTCTCTCTCTCTCTCCCCCGC50.12830382345393893No Hit
GCCCTCTCTCTCTCTCTCTCCCCCGC50.12830382345393893No Hit
CTCGTTTTGAGGTGGGTTTCCCGCTTAGATGCTTTCAGCGGTTATCCCGT50.12830382345393893No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACCAAGACTCAAATCTAGGG50.12830382345393893Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCGTCACCAAGACTCAAATCTAGGG50.12830382345393893Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGGGGGGG50.12830382345393893TruSeq Adapter, Index 2 (96% over 32bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC50.12830382345393893No Hit
GATCGGAAGAGCACGTCTGAACTCCAGTCACCAAGACTCAAATCTGGGGG50.12830382345393893TruSeq Adapter, Index 7 (95% over 23bp)
GATCGGAAGAGCACACGTCGAACTCCAGTCACCAAGACTCAAATCTAGGG40.10264305876315115No Hit
GCCCCCCCGGCAGCAGC40.10264305876315115No Hit
CCCCGGGGAGGGGGGAGGACGGGG40.10264305876315115No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGCAGC40.10264305876315115No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGAG40.10264305876315115No Hit
GGGAGAGAGAGAGAGAGAGGGCGCGAAG40.10264305876315115No Hit
CGGGGGAGAGAGAGAGAGAGAGGGCGCGAAG40.10264305876315115No Hit
GATCGGAAGAGACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGGGG40.10264305876315115TruSeq Adapter, Index 7 (95% over 24bp)
GATCGGAAGACACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGGGG40.10264305876315115TruSeq Adapter, Index 2 (96% over 25bp)
GGGGGAGAGAGAGAGAGAGGGCGCGAAG40.10264305876315115No Hit
GAGAGAGAGAGAGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA40.10264305876315115No Hit
GCCCTCTCTCTCTCTCTCTCCCCC40.10264305876315115No Hit
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG40.10264305876315115No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGGGGGGGG40.10264305876315115TruSeq Adapter, Index 2 (96% over 31bp)
GGGGAGAGAGAGAGAGAGAGGGCGCGAAG40.10264305876315115No Hit
GAGAGAGAGAGAGGGCGCGAAG40.10264305876315115No Hit
GATCGGAAGAGCACACGTCTGAAACTCCAGTCACCAAGACTCAAATCTAG40.10264305876315115Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CTTCGGCGCAGAAACCCTTATTTAGACCAGTGAGCTGTTACGCTTTCTTT40.10264305876315115No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)