Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 29323962 total bases: 4427918262 Q20 bases: 4247853633(95.9334%) Q30 bases: 4004976624(90.4483%) Read2 before filtering: total reads: 29323962 total bases: 4427918262 Q20 bases: 4312438258(97.392%) Q30 bases: 4070176037(91.9208%) Read1 after filtering: total reads: 28784902 total bases: 2999306443 Q20 bases: 2985393009(99.5361%) Q30 bases: 2923195078(97.4624%) Read2 after filtering: total reads: 28784902 total bases: 3004301796 Q20 bases: 2985597594(99.3774%) Q30 bases: 2917281593(97.1035%) Filtering result: reads passed filter: 57569804 reads failed due to low quality: 607852 reads failed due to too many N: 1802 reads failed due to too short: 468466 reads with adapter trimmed: 51772731 bases trimmed due to adapters: 2714891854 Duplication rate: 36.642% Insert size peak (evaluated by paired-end reads): 96 JSON report: aih-tih-sc-d5fda9-R1_A23YTGFLT4_1.fastp.json HTML report: aih-tih-sc-d5fda9-R1_A23YTGFLT4_1.fastp.html fastp --in1 aih-tih-sc-d5fda9-R1_A23YTGFLT4_1_1.fastq.gz --in2 aih-tih-sc-d5fda9-R1_A23YTGFLT4_1_2.fastq.gz --out1 aih-tih-sc-d5fda9-R1_A23YTGFLT4_1_1.fastp.fastq.gz --out2 aih-tih-sc-d5fda9-R1_A23YTGFLT4_1_2.fastp.fastq.gz --json aih-tih-sc-d5fda9-R1_A23YTGFLT4_1.fastp.json --html aih-tih-sc-d5fda9-R1_A23YTGFLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 62 seconds