Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 186338622 total bases: 28137131922 Q20 bases: 27399387338(97.378%) Q30 bases: 26190297816(93.0809%) Read2 before filtering: total reads: 186338622 total bases: 28137131922 Q20 bases: 27481195338(97.6688%) Q30 bases: 26131942263(92.8735%) Read1 after filtering: total reads: 183773725 total bases: 21177459093 Q20 bases: 21086413010(99.5701%) Q30 bases: 20662193282(97.5669%) Read2 after filtering: total reads: 183773725 total bases: 21209883776 Q20 bases: 21062377764(99.3045%) Q30 bases: 20538078496(96.8326%) Filtering result: reads passed filter: 367547450 reads failed due to low quality: 4768698 reads failed due to too many N: 12570 reads failed due to too short: 348526 reads with adapter trimmed: 285981141 bases trimmed due to adapters: 13223376426 Duplication rate: 38.3239% Insert size peak (evaluated by paired-end reads): 100 JSON report: aih-tih-sc-607ec3-R1_A23YTGFLT4_1.fastp.json HTML report: aih-tih-sc-607ec3-R1_A23YTGFLT4_1.fastp.html fastp --in1 aih-tih-sc-607ec3-R1_A23YTGFLT4_1_1.fastq.gz --in2 aih-tih-sc-607ec3-R1_A23YTGFLT4_1_2.fastq.gz --out1 aih-tih-sc-607ec3-R1_A23YTGFLT4_1_1.fastp.fastq.gz --out2 aih-tih-sc-607ec3-R1_A23YTGFLT4_1_2.fastp.fastq.gz --json aih-tih-sc-607ec3-R1_A23YTGFLT4_1.fastp.json --html aih-tih-sc-607ec3-R1_A23YTGFLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 394 seconds