Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 149616053 total bases: 22592024003 Q20 bases: 21892449551(96.9034%) Q30 bases: 20827871511(92.1913%) Read2 before filtering: total reads: 149616053 total bases: 22592024003 Q20 bases: 22101835238(97.8303%) Q30 bases: 21052701578(93.1864%) Read1 after filtering: total reads: 147587887 total bases: 16281367559 Q20 bases: 16212467664(99.5768%) Q30 bases: 15895479259(97.6299%) Read2 after filtering: total reads: 147587887 total bases: 16305711153 Q20 bases: 16204363539(99.3785%) Q30 bases: 15834317182(97.109%) Filtering result: reads passed filter: 295175774 reads failed due to low quality: 3428682 reads failed due to too many N: 9682 reads failed due to too short: 617968 reads with adapter trimmed: 247401165 bases trimmed due to adapters: 12078437388 Duplication rate: 37.3953% Insert size peak (evaluated by paired-end reads): 97 JSON report: aih-tih-sc-a558c6-R1_A23YTGFLT4_1.fastp.json HTML report: aih-tih-sc-a558c6-R1_A23YTGFLT4_1.fastp.html fastp --in1 aih-tih-sc-a558c6-R1_A23YTGFLT4_1_1.fastq.gz --in2 aih-tih-sc-a558c6-R1_A23YTGFLT4_1_2.fastq.gz --out1 aih-tih-sc-a558c6-R1_A23YTGFLT4_1_1.fastp.fastq.gz --out2 aih-tih-sc-a558c6-R1_A23YTGFLT4_1_2.fastp.fastq.gz --json aih-tih-sc-a558c6-R1_A23YTGFLT4_1.fastp.json --html aih-tih-sc-a558c6-R1_A23YTGFLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 283 seconds