Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 195659080 total bases: 29544521080 Q20 bases: 28660730821(97.0086%) Q30 bases: 27404679392(92.7572%) Read2 before filtering: total reads: 195659080 total bases: 29544521080 Q20 bases: 28827292875(97.5724%) Q30 bases: 27361881973(92.6124%) Read1 after filtering: total reads: 192722592 total bases: 22178821289 Q20 bases: 22077207226(99.5418%) Q30 bases: 21624773463(97.5019%) Read2 after filtering: total reads: 192722592 total bases: 22219468464 Q20 bases: 22062554016(99.2938%) Q30 bases: 21519053331(96.8477%) Filtering result: reads passed filter: 385445184 reads failed due to low quality: 4996304 reads failed due to too many N: 13554 reads failed due to too short: 863118 reads with adapter trimmed: 299278430 bases trimmed due to adapters: 13928820404 Duplication rate: 46.2782% Insert size peak (evaluated by paired-end reads): 98 JSON report: aih-tih-sc-31d31f-R1_A23YTGFLT4_1.fastp.json HTML report: aih-tih-sc-31d31f-R1_A23YTGFLT4_1.fastp.html fastp --in1 aih-tih-sc-31d31f-R1_A23YTGFLT4_1_1.fastq.gz --in2 aih-tih-sc-31d31f-R1_A23YTGFLT4_1_2.fastq.gz --out1 aih-tih-sc-31d31f-R1_A23YTGFLT4_1_1.fastp.fastq.gz --out2 aih-tih-sc-31d31f-R1_A23YTGFLT4_1_2.fastp.fastq.gz --json aih-tih-sc-31d31f-R1_A23YTGFLT4_1.fastp.json --html aih-tih-sc-31d31f-R1_A23YTGFLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 424 seconds