[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (7339516) splicing reads. 226982 splicing junctions. 175774 known splicing junctions. 51208 novel splicing junctions.
sampling 10% (14679033) splicing reads. 276693 splicing junctions. 190363 known splicing junctions. 86330 novel splicing junctions.
sampling 15% (22018549) splicing reads. 312637 splicing junctions. 198418 known splicing junctions. 114219 novel splicing junctions.
sampling 20% (29358066) splicing reads. 341430 splicing junctions. 203808 known splicing junctions. 137622 novel splicing junctions.
sampling 25% (36697583) splicing reads. 365700 splicing junctions. 207792 known splicing junctions. 157908 novel splicing junctions.
sampling 30% (44037099) splicing reads. 386659 splicing junctions. 210983 known splicing junctions. 175676 novel splicing junctions.
sampling 35% (51376616) splicing reads. 404686 splicing junctions. 213467 known splicing junctions. 191219 novel splicing junctions.
sampling 40% (58716133) splicing reads. 420743 splicing junctions. 215556 known splicing junctions. 205187 novel splicing junctions.
sampling 45% (66055649) splicing reads. 434862 splicing junctions. 217160 known splicing junctions. 217702 novel splicing junctions.
sampling 50% (73395166) splicing reads. 447525 splicing junctions. 218589 known splicing junctions. 228936 novel splicing junctions.
sampling 55% (80734683) splicing reads. 458913 splicing junctions. 219833 known splicing junctions. 239080 novel splicing junctions.
sampling 60% (88074199) splicing reads. 468968 splicing junctions. 220886 known splicing junctions. 248082 novel splicing junctions.
sampling 65% (95413716) splicing reads. 478041 splicing junctions. 221819 known splicing junctions. 256222 novel splicing junctions.
sampling 70% (102753233) splicing reads. 486081 splicing junctions. 222581 known splicing junctions. 263500 novel splicing junctions.
sampling 75% (110092749) splicing reads. 493224 splicing junctions. 223312 known splicing junctions. 269912 novel splicing junctions.
sampling 80% (117432266) splicing reads. 499679 splicing junctions. 223907 known splicing junctions. 275772 novel splicing junctions.
sampling 85% (124771783) splicing reads. 505124 splicing junctions. 224411 known splicing junctions. 280713 novel splicing junctions.
sampling 90% (132111299) splicing reads. 509923 splicing junctions. 224899 known splicing junctions. 285024 novel splicing junctions.
sampling 95% (139450816) splicing reads. 513858 splicing junctions. 225259 known splicing junctions. 288599 novel splicing junctions.
sampling 100% (146790333) splicing reads. 517158 splicing junctions. 225537 known splicing junctions. 291621 novel splicing junctions.