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Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e8/d2521bce0b275263ad0739d6d32400/aih-tih-sc-1274d4-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
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Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a3/6c165ed8a637a13dec651605e0ee3f/aih-tih-sc-1274d4-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam
==> STAGING COMPLETE (7 inputs)
20:00:20 : INFO : -reading targets list: ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-mapping reads to genes
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[7700000], rate=8716981.13/min 20:01:28 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file aih-tih-sc-1274d4-R1_A23YTGFLT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > aih-tih-sc-1274d4-R1_A23YTGFLT4_1.cancer.introns.prelim
Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18.
-aih-tih-sc-1274d4-R1_A23YTGFLT4_1.introns: identified 6129 cancer introns
20:01:29 : INFO : Execution Time = 0.02 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file aih-tih-sc-1274d4-R1_A23YTGFLT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > aih-tih-sc-1274d4-R1_A23YTGFLT4_1.cancer.introns.prelim
20:01:29 : INFO : Running: touch /tmp/nxf.m5Tq7VqLBB/aih-tih-sc-1274d4-R1_A23YTGFLT4_1.chckpts/prelim_introns.ok
20:01:29 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates aih-tih-sc-1274d4-R1_A23YTGFLT4_1.cancer.introns.prelim --min_total_reads 5 > aih-tih-sc-1274d4-R1_A23YTGFLT4_1.cancer.introns
20:01:30 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates aih-tih-sc-1274d4-R1_A23YTGFLT4_1.cancer.introns.prelim --min_total_reads 5 > aih-tih-sc-1274d4-R1_A23YTGFLT4_1.cancer.introns
20:01:30 : INFO : Running: touch /tmp/nxf.m5Tq7VqLBB/aih-tih-sc-1274d4-R1_A23YTGFLT4_1.chckpts/introns_filtered.ok
20:01:30 : INFO : -found 2073 cancer introns
20:01:30 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns aih-tih-sc-1274d4-R1_A23YTGFLT4_1.introns --cancer_introns aih-tih-sc-1274d4-R1_A23YTGFLT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed aih-tih-sc-1274d4-R1_A23YTGFLT4_1.introns.for_IGV.bed
20:01:30 : INFO : Creating the BED File.
20:01:43 : INFO : Saving Bed File as aih-tih-sc-1274d4-R1_A23YTGFLT4_1.introns.for_IGV.bed
20:01:43 : INFO : Execution Time = 0.22 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns aih-tih-sc-1274d4-R1_A23YTGFLT4_1.introns --cancer_introns aih-tih-sc-1274d4-R1_A23YTGFLT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed aih-tih-sc-1274d4-R1_A23YTGFLT4_1.introns.for_IGV.bed
20:01:43 : INFO : Running: touch /tmp/nxf.m5Tq7VqLBB/aih-tih-sc-1274d4-R1_A23YTGFLT4_1.chckpts/intron_igv_bed.ok
20:01:43 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed aih-tih-sc-1274d4-R1_A23YTGFLT4_1.introns.for_IGV.bed --bam aih-tih-sc-1274d4-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam --output_prefix aih-tih-sc-1274d4-R1_A23YTGFLT4_1
[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1274d4-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
20:22:48 : INFO : Execution Time = 21.09 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed aih-tih-sc-1274d4-R1_A23YTGFLT4_1.introns.for_IGV.bed --bam aih-tih-sc-1274d4-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam --output_prefix aih-tih-sc-1274d4-R1_A23YTGFLT4_1
20:22:48 : INFO : Running: touch /tmp/nxf.m5Tq7VqLBB/aih-tih-sc-1274d4-R1_A23YTGFLT4_1.chckpts/reads_alignments_extracted.ok
20:22:49 : INFO : Running: samtools sort -o aih-tih-sc-1274d4-R1_A23YTGFLT4_1.cancer_intron_reads.sorted.bam aih-tih-sc-1274d4-R1_A23YTGFLT4_1.cancer_intron_reads.bam
20:22:49 : INFO : Execution Time = 0.01 minutes. CMD: samtools sort -o aih-tih-sc-1274d4-R1_A23YTGFLT4_1.cancer_intron_reads.sorted.bam aih-tih-sc-1274d4-R1_A23YTGFLT4_1.cancer_intron_reads.bam
20:22:49 : INFO : Running: touch /tmp/nxf.m5Tq7VqLBB/aih-tih-sc-1274d4-R1_A23YTGFLT4_1.chckpts/sort_cancer_intron_reads.ok
20:22:49 : INFO : Running: samtools sort -o aih-tih-sc-1274d4-R1_A23YTGFLT4_1.gene_reads.sorted.bam aih-tih-sc-1274d4-R1_A23YTGFLT4_1.gene_reads.bam
[bam_sort_core] merging from 74 files and 1 in-memory blocks...