+ mkdir aih-tih-sc-01eb49-R1_A23YTGFLT4_1 + all_qc -bam aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-01eb49-R1_A23YTGFLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai 0it [00:00, ?it/s] 155267it [00:00, 1552512.32it/s] 322720it [00:00, 1624237.50it/s] 493185it [00:00, 1660916.88it/s] 666295it [00:00, 1688495.27it/s] 843997it [00:00, 1720385.17it/s] 1019184it [00:00, 1730979.12it/s] 1194308it [00:00, 1737579.85it/s] 1372782it [00:00, 1752566.34it/s] 1548330it [00:00, 1753450.23it/s] 1723878it [00:01, 1754052.82it/s] 1899284it [00:01, 1743599.14it/s] 2073658it [00:01, 1703061.65it/s] 2244164it [00:01, 1664882.63it/s] 2410916it [00:01, 1649036.13it/s] 2575994it [00:01, 1592542.18it/s] 2735657it [00:01, 1526724.22it/s] 2901553it [00:01, 1564077.60it/s] 3076990it [00:01, 1618786.44it/s] 3245565it [00:01, 1626958.97it/s] 3417061it [00:02, 1652663.50it/s] 3588678it [00:02, 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1742543.55it/s] 8721162it [00:05, 1749590.44it/s] 8896622it [00:05, 1751069.59it/s] 9071740it [00:05, 1680490.71it/s] 9240403it [00:05, 1580747.52it/s] 9399927it [00:05, 1571549.80it/s] 9570219it [00:05, 1608949.85it/s] 9739438it [00:05, 1633001.94it/s] 9910590it [00:05, 1655932.47it/s] 10079918it [00:06, 1666912.75it/s] 10251746it [00:06, 1682097.78it/s] 10423805it [00:06, 1693408.79it/s] 10596692it [00:06, 1703956.43it/s] 10770284it [00:06, 1713476.71it/s] 10942575it [00:06, 1716131.96it/s] 11116993it [00:06, 1724504.56it/s] 11289504it [00:06, 1712445.21it/s] 11460809it [00:06, 1712522.55it/s] 11632104it [00:06, 1709532.74it/s] 11804296it [00:07, 1713210.27it/s] 11975640it [00:07, 1711397.62it/s] 12146853it [00:07, 1711550.06it/s] 12318268it [00:07, 1712254.38it/s] 12489801it [00:07, 1713150.51it/s] 12661122it [00:07, 1708397.20it/s] 12831969it [00:07, 1707216.26it/s] 13004669it [00:07, 1713110.93it/s] 13177294it [00:07, 1717023.27it/s] 13349001it [00:07, 1716054.53it/s] 13523599it [00:08, 1724995.79it/s] 13696221it [00:08, 1725333.63it/s] 13868757it [00:08, 1693641.52it/s] 14038252it [00:08, 1693579.92it/s] 14207701it [00:08, 1691801.40it/s] 14261288it [00:08, 1682189.88it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md.bai + merge_qc -folders aih-tih-sc-01eb49-R1_A23YTGFLT4_1/aih-tih-sc-01eb49-R1_A23YTGFLT4_1.md -output aih-tih-sc-01eb49-R1_A23YTGFLT4_1/aih-tih-sc-01eb49-R1_A23YTGFLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-01eb49-R1_A23YTGFLT4_1/aih-tih-sc-01eb49-R1_A23YTGFLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-01eb49-R1_A23YTGFLT4_1/aih-tih-sc-01eb49-R1_A23YTGFLT4_1_full_basic_stats.tsv