[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-da06ce-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	369043498
Known Splicing Events:	366148056
Partial Novel Splicing Events:	1758184
Novel Splicing Events:	1040665
Filtered Splicing Events:	96593

Total splicing  Junctions:	447988
Known Splicing Junctions:	254332
Partial Novel Splicing Junctions:	116863
Novel Splicing Junctions:	76793

===================================================================
Create BED file ...
Create Interact file ...